The catalytic activity of cytochrome P450(cam) towards styrene oxidation is increased by site-specific mutagenesis

FEBS Letters

Volumn 405, Issue 2, 1997, Pages 153-156

  Nickerson, Darren P ^a   Harford Cross, Charles F ^a   Fulcher, Sarah R ^a   Wong, Luet Lok ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Epoxidation; Monooxygenase; Mutagenesis; P450; Stereoselectivity; Styrene

Indexed keywords

CYTOCHROME P450; STYRENE; UNSPECIFIC MONOOXYGENASE;

ARTICLE; CATALYSIS; EPOXIDATION; MUTAGENESIS; NONHUMAN; OXIDATION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STEREOSPECIFICITY;

BINDING SITES; CAMPHOR 5-MONOOXYGENASE; MUTAGENESIS, SITE-DIRECTED; MUTATION; OXIDATION-REDUCTION; PROTEIN ENGINEERING; SPECTROPHOTOMETRY; STYRENE; STYRENES; SUBSTRATE SPECIFICITY;

EID: 0030992065     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(97)00174-9     Document Type: Article

References (27)

1. Ortiz de Montellano, P.R., Ed. (1986) Cytochrome P-450: Structure, Mechanism and Biochemistry, Plenum Press, New York.
2. Guengerich, F.P. (1991) J. Biol. Chem. 266, 10019-10022.
3. Ortiz de Montellano, P.R., Ed. (1995) Cytochrome P-450: Structure, Mechanism and Biochemistry (2nd edn.), Plenum Press, New York.
4. Gunsalus, I.C. and Wagner, G.C. (1978) Methods Enzymol. 52, 166-188.
5. Poulos, T.L., Finzel, B.C. and Howard, A.J. (1987) J. Mol. Biol. 105, 687-700.
6. Atkins, W.M. and Sligar, S.G. (1988) J. Biol. Chem. 263, 18842-18849.
7. Atkins, W.M. and Sligar, S.G. (1989) J. Am. Chem. Soc. 111, 2715-2717.
8. Loida, P.J. and Sligar, S.G. (1993) Biochemistry 32, 11530-11538.
9. Fruetel, J.A., Collins, J.R., Camper, D.L., Loew, G.H. and Ortiz de Montellano, P.R. (1992) J. Am. Chem. Soc. 114, 6987-6993.
10. Jones, J.P., Trager, W.F. and Carlson, T.J. (1993) J. Am. Chem. Soc. 115, 381-387.
11. Watanabe, Y. and Ishimura, Y. (1989) J. Am. Chem. Soc. 111, 410-411.
12. Grayson, D.A., Tewari, Y.B., Mayhew, M.P., Vilker, V.L. and Goldberg, R.N. (1996) Arch. Biochem. Biophys. 332, 239-247.
13. Filipovic, D., Paulsen, M.D., Loida, P.J., Sligar, S.G. and Ornstein, R.L. (1992) Biochem. Biophys. Res. Comm. 189, 488-495.
14. Fowler, S.M., England, P.A., Westlake, A.C.G., Rouch, D.A., Nickerson, D.P., Blunt, C., Braybrook, D., West, S., Wong, L.-L. and Flitsch, S.L. (1994) J. Chem. Soc., Chem. Commun. 2761.
15. Unger, B.P., Gunsalus, I.C. and Sligar, S.G. (1986) J. Biol. Chem. 261, 1158-1163.
16. Peterson, J.A., Lorence, M.C. and Amarneh, B. (1990) J. Biol. Chem. 265, 6066-6073.
17. Koga, H., Yamaguchi, E., Matsunaga, K., Aramaki, H. and Horiuchi, T. (1989) J. Biochem. 106, 831-836.
18. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual (2nd edn.), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
19. Kunkel, T.A. (1985) Proc. Natl. Acad. Sci. USA. 82, 488-492.
20. Peterson, J.A. (1971) Arch. Biochem. Biophys. 144, 678-693.
21. Poulos, T.L., Finzel, B.C. and Howard, A.J. (1986) Biochemistry 25, 5314-5322.
22. Sligar, S.G. (1976) Biochemistry 15, 5399-5406.
23. Brewer, C.B. and Peterson, J.A. (1988) J. Biol. Chem. 263, 791-798.
24. Fisher, M.T. and Sligar, S.G. (1985) J. Am. Chem. Soc. 107, 5018-5019.
25. Harris, D. and Loew, G. (1993) J. Am. Chem. Soc. 115, 8775-8779.
26. Raag, R., Li, H., Jones, B.C. and Poulos, T.L. (1993) Biochemistry, 32, 4571-4578.
27. Mueller, E.J., Loida, P.J. and Sligar, S.G. (1995) in: Cytochrome P450: Structure, Mechanism, and Biochemistry (Ortiz de Montellano, P.R., Ed.), 2nd edn., pp. 83-124, Plenum Press, New York, and references therein.