Regio- and stereospecificity of filipin hydroxylation sites revealed by crystal structures of cytochrome P450 105P1 and 105D6 from Streptomyces avermitilis

Journal of Biological Chemistry

Volumn 285, Issue 22, 2010, Pages 16844-16853

  Xu, Lian Hua ^a   Fushinobu, Shinya ^a   Takamatsu, Satoshi ^b   Wakagi, Takayoshi ^a   Ikeda, Haruo ^b   Shoun, Hirofumi ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b KITASATO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ALKYL SIDE CHAINS; CYTOCHROME P450; DIAGNOSTIC TOOLS; DISTAL POCKET; HEME IRON; HYDROXYLASES; LIGAND-FREE; LOOP REGIONS; MACROLIDES; MOLECULAR DIVERSITY; POLYENE MACROLIDE; POLYKETIDES; REGIOSPECIFICITY; SEQUENCE SIMILARITY; STEREOSPECIFICITY; STERIC HINDRANCES; STREPTOMYCES AVERMITILIS; SUBSTRATE BINDING; SUBSTRATE-FREE; WATER MOLECULE;

ANTIBIOTICS; BINDING ENERGY; BIOCHEMISTRY; CATALYSTS; ENZYMES; HYDROCARBONS; HYDROXYLATION; PORPHYRINS; SUBSTRATES;

CRYSTAL STRUCTURE;

CYTOCHROME P450; CYTOCHROME P450 105D6; CYTOCHROME P450 105P1; FILIPIN; HEME; IRON; POLYOL; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME BINDING; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; HYDROPHOBICITY; HYDROXYLATION; NONHUMAN; PRIORITY JOURNAL; STEREOSPECIFICITY; STREPTOMYCES; STREPTOMYCES AVERMITILIS;

AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; CRYSTALLOGRAPHY, X-RAY; CYTOCHROME P-450 ENZYME SYSTEM; FILIPIN; HEME; KINETICS; LIGANDS; MACROLIDES; MICROBIAL SENSITIVITY TESTS; MOLECULAR CONFORMATION; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, TERTIARY; STEREOISOMERISM; STREPTOMYCES; SUBSTRATE SPECIFICITY;

STREPTOMYCES AVERMITILIS;

EID: 77952754570     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M109.092460     Document Type: Article

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