Construction of a novel expression vector in Pseudonocardia autotrophica and its application to efficient biotransformation of compactin to pravastatin, a specific HMG-CoA reductase inhibitor

Biochemical and Biophysical Research Communications

Volumn 404, Issue 1, 2011, Pages 511-516

  Fujii, Yoshikazu ^a,c   Norihisa, Koji ^a   Fujii, Tadashi ^a   Aritoku, Yasuhide ^a   Kagawa, Yusuke ^c   Sallam, Khalid Ibrahim ^b   Johdo, Osamu ^a   Arisawa, Akira ^a   Tamura, Tomohiro ^b,c  

^a MERCIAN CORPORATION   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^c HOKKAIDO UNIVERSITY   (Japan)

Author keywords

Acetone inducible promoter; Biotransformation; Pravastatin; Pseudonocardia autotrophica; Rolling circle type replication

Indexed keywords

ACETONE; ALDEHYDE DEHYDROGENASE; COMPACTIN; CYTOCHROME P450; CYTOCHROME P450 OXYGENASE; PLASMID VECTOR; PRAVASTATIN; UNCLASSIFIED DRUG;

ACTINOBACTERIA; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM ISOLATION; CONTROLLED STUDY; DRUG ACCUMULATION; DRUG TRANSFORMATION; ELECTROPORATION; EXPRESSION VECTOR; GENE EXPRESSION; HYPERCHOLESTEROLEMIA; IMMUNE RESPONSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN FOLDING; PSEUDONOCARDIA AUTOTROPHICA; SEQUENCE HOMOLOGY;

ACETONE; BASE SEQUENCE; BIOTRANSFORMATION; CLONING, MOLECULAR; CYTOCHROME P-450 ENZYME SYSTEM; GENE EXPRESSION; GENETIC ENGINEERING; GENETIC VECTORS; HYDROXYMETHYLGLUTARYL-COA REDUCTASE INHIBITORS; INDUSTRIAL MICROBIOLOGY; LOVASTATIN; MOLECULAR SEQUENCE DATA; PLASMIDS; PRAVASTATIN; PROMOTER REGIONS, GENETIC; REPLICATION ORIGIN; STREPTOMYCES;

PSEUDONOCARDIA; PSEUDONOCARDIA AUTOTROPHICA;

EID: 78650909062     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.12.013     Document Type: Article

References (16)

1. Kieser T., Bibb M.J., Buttner M.J., Chater K.F., Hopwood D.A. Practical Streptomyces Genetics 2000, John Innes Foundation, Norwich.
2. 3. J. Ferment. Bioeng. 1994, 78:380-382.
3. 3 hydroxylase from Pseudonocardia autotrophica. Biochem. Biophys. Res. Commun. 2009, 385:170-175.
4. Lamb D.C., Skaug T., Song H.L., Jackson C.J., Podust L.M., Waterman M.R., et al. The cytochrome P450 complement (CYPome) of Streptomyces coelicolor A3(2). J. Biol. Chem. 2002, 277:24000-24005.
5. Abe Y., Baba S., Suzuki T., Ono C., Iwamoto K. Molecular basis of ML-236B production in the high-producing mutant No. 41520 of Penicillium citrinum. J. Gen. Appl. Microbiol. 2004, 50:169-176.
6. Watanabe I., Nara F., Serizawa N. Cloning, characterization and expression of the gene encoding cytochrome P-450sca-2 from Streptomyces carbophilus involved in production of pravastatin, a specific HMG-CoA reductase inhibitor. Gene 1995, 163:81-85.
7. Peng Y., Demain A.L. Bioconversion of compactin to pravastatin by Actinomadura sp. ATCC 55678. J. Mol. Catal. B Enzym. 2000, 10:151-156.
8. Park J.W., Lee J.K., Kwon T.J., Yi D.H., Kim Y.J., Moon S.H., et al. Bioconversion of compactin into pravastatin by Streptomyces sp. Biotechnol. Lett. 2003, 25:1827-1831.
9. Chen C.H., Hu H.Y., Cho Y.C., Hsu W.H. Screening of compactin-resistant microorganisms capable of converting compactin to pravastatin. Curr. Microbiol. 2006, 53:108-112.
10. T. Fujii, S. Hirosue, Y. Aritoku, Novel polypeptide, DNA encoding the polypeptide and use thereof, WO2002/099109.
11. Sambrook J., Russell D.W. Molecular Cloning: A Laboratory Manual 2001, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. 3rd edn.
12. Sallam K.I., Tamura N., Tamura T. A multipurpose transposon-based vector system mediates protein expression in Rhodococcus erythropolis. Gene 2007, 386:173-182.
13. Shao Z., Dick W.A., Behki R.M. An improved Escherichia coli-Rhodococcus shuttle vector and plasmid transformation in Rhodococcus spp. using electroporation. Lett. Appl. Microbiol. 1995, 21:261-266.
14. Omura T., Sato R. The carbon monoxide-binding pigment of liver microsomes. J. Biol. Chem. 1964, 239:2370-2378.
15. Khan A.S. Plasmid rolling-circle replication: highlights of two decades of research. Plasmid 2005, 53:126-136.
16. Fujii T., Fujii Y., Machida K., Ochiai A., Ito M. Efficient biotransformations using Escherichia coli with tolC acrAB mutations expressing cytochrome P450 genes. Biosci. Biotechnol. Biochem. 2009, 73:805-810.