Ligation-independent cloning and self-cleaving intein as a tool for high-throughput protein purification

Protein Expression and Purification

Volumn 91, Issue 2, 2013, Pages 169-174

  Warren, Tiana D ^a   Coolbaugh, Michael J ^b   Wood, David W ^b  

^a Johns Hopkins University   (United States)

^b The Ohio State University   (United States)

Author keywords

Intein; Ligation independent cloning; Molecular cloning; Protein purification; Self cleaving affinity tag

Indexed keywords

CHITIN; HYBRID PROTEIN; INTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CHEMISTRY; HIGH THROUGHPUT SCREENING; ISOLATION AND PURIFICATION; LIGATION-INDEPENDENT CLONING; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN PURIFICATION; SELF-CLEAVING AFFINITY TAG;

INTEIN; LIGATION-INDEPENDENT CLONING; MOLECULAR CLONING; PROTEIN PURIFICATION; SELF-CLEAVING AFFINITY TAG;

BASE SEQUENCE; CHITIN; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HIGH-THROUGHPUT SCREENING ASSAYS; INTEINS; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS;

EID: 84883522715     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2013.08.006     Document Type: Article

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