Recombinant protein purification by unit 26.4 self-cleaving elastin-like polypeptide fusion tag

Current Protocols in Protein Science

Volumn , Issue SUPPL.58, 2009, Pages

  Wu, Wan Yi ^a   Fong, Baley A ^a   Gilles, Allison G ^a   Wood, David W ^a  

^a PRINCETON UNIVERSITY   (United States)

Author keywords

Elastin like polypeptide; Gateway cloning; Intein; Nonchromatographic bioseparations; Protein purification

Indexed keywords

AGAR; AMPICILLIN; BUFFER; DNA; ELASTIN; KANAMYCIN; POLYPEPTIDE; REAGENT; RECOMBINANT PROTEIN; ELASTIN LIKE POLYPEPTIDE; HYBRID PROTEIN; UNCLASSIFIED DRUG;

ANALYTIC METHOD; CENTRIFUGATION; CLONING VECTOR; CYTOLYSIS; GENE TARGETING; MOLECULAR CLONING; PH; PLASMID; POLYMERASE CHAIN REACTION; PRECIPITATION; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RECOMBINANT DNA TECHNOLOGY; RESTRICTION MAPPING; REVIEW; TEMPERATURE; ARTICLE; BIOSEPARATION; CHEMICAL PROCEDURES; CHROMATOGRAPHY; CLINICAL PROTOCOL; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; NONHUMAN; PROTEIN TARGETING; TEMPERATURE SENSITIVITY; THERMODYNAMICS;

CLONING, MOLECULAR; ELASTIN; ESCHERICHIA COLI; GENETIC VECTORS; HYDROGEN-ION CONCENTRATION; PROTEINS; RECOMBINANT FUSION PROTEINS;

EID: 75749129871     PISSN: 19343655     EISSN: 19343663     Source Type: Journal    
DOI: 10.1002/0471140864.ps2604s58     Document Type: Review

References (27)

1. Banki, M.R., Feng, L., and Wood, D.W. 2005. Simple bioseparations using self-cleaving elastinlike polypeptide tags. Nat. Methods 2:659-661.
2. Banki,M.R., Gerngross, U., andWood, D.W. 2005. Novel and economical purification of recombinant proteins: intein-mediated protein purification using in vivo polyhydroxybutyrate (PHB) matrix association. Protein Sci. 14:1387-1395.
3. Banki, M.R. and Wood, D.W. 2005. Inteins and affinity resin substitutes for protein purification and scale up. Microb. Cell Fact 4:32.
4. Bushman, W., Thompson, J.F., Vargas, L., and Landy, A. 1985. Control of directionality in lambda site specific recombination. Science 230:906-911.
5. Chong, S., Mersha, F.B., Comb, D.G., Scott, M.E., Landry, D., Vence, L.M., Perler, F.B., Benner, J., Kucera, R.B., Hirvonen, C.A., Pelletier, J.J., Paulus, H., and Xu, M.Q. 1997. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene 192:271-281.
6. Chong, S., Montello, G.E., Zhang, A., Cantor, E.J., Liao,W., Xu,M.Q., and Benner, J. 1998. Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step. Nucleic Acids Res. 26:5109-5115.
7. Davis, E.O., Jenner, P.J., Brooks, P.C., Colston, M.J., and Sedgwick, S.G. 1992. Protein splicing in the maturation of M. tuberculosis recA protein: A mechanism for tolerating a novel class of intervening sequence. Cell 71:201-210.
8. Fong, B.A.,Wu,W.Y., andWood, D.W. 2009. Optimization of ELP-intein mediated protein purification by salt substitution. Protein Expr. Purif. 66:198-202.
9. Ge, X., Yang, D.S., Trabbic-Carlson, K., Kim, B., Chilkoti, A., and Filipe, C.D. 2005. Selfcleavable stimulus responsive tags for protein purification without chromatography. J. Am. Chem. Soc. 127:11228-11229.
10. Gillies, A.R., Hsii, J.F., Oak, S., and Wood, D.W. 2008. Rapid cloning and purification of proteins: Gateway vectors for protein purification by self-cleaving tags. Biotechnol. Bioeng. 101:229-240.
11. Hiraga, K., Derbyshire, V., Dansereau, J.T., Van Roey, P., and Belfort, M. 2005. Minimization and stabilization of the Mycobacterium tuberculosis recA intein. J. Mol. Biol. 354:916-926.
12. Liu, X.Q. 2000. Protein-splicing intein: Genetic mobility, origin, and evolution. Annu. Rev. Genet. 34:61-76.
13. Luan, C.H., Parker, T.M., Gowda, D.C., and Urry, D.W. 1992. Hydrophobicity of amino acid residues: Differential scanning calorimetry and synthesis of the aromatic analogues of the polypentapeptide of elastin. Biopolymers 32:1251-1261.
14. Meyer, D.E. and Chilkoti, A. 1999. Purification of recombinant proteins by fusion with thermally-responsive polypeptides. Nat. Biotechnol. 17:1112-1115.
15. Meyer, D.E. and Chilkoti, A. 2004. Quantification of the effects of chain length and concentration on the thermal behavior of elastin-like polypeptides. Biomacromolecules 5:846-851.
16. Patel, J., Zhu, H., Menassa, R., Gyenis, L., Richman, A., and Brandle, J. 2007. Elastinlike polypeptide fusions enhance the accumulation of recombinant proteins in tobacco leaves. Transgenic Res. 16:239-249.
17. Perler, F.B., Davis, E.O., Dean, G.E., Gimble, F.S., Jack, W.E., Neff, N., Noren, C.J., Thorner, J., and Belfort,M. 1994. Protein splicing elements: inteins and exteins: A definition of terms and recommended nomenclature. Nucleic Acids Res. 22:1125-1127.
18. Reguera, J., Urry, D.W., Parker, T.M., McPherson, D.T., and Rodriguez-Cabello, J.C. 2007. Effect of NaCl on the exothermic and endothermic components of the inverse temperature transition of a model elastin-like polymer. Biomacromolecules 8:354-358.
19. Southworth, M.W., Amaya, K., Evans, T.C., Xu, M.Q., and Perler, F.B. 1999. Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein. Biotechniques 27:110-120.
20. Terpe, K. 2003. Overview of tag protein fusions: From molecular and biochemical fundamentals to commercial systems. Appl. Microbiol. Biotechnol. 60:523-533.
21. Trabbic-Carlson, K., Liu, L., Kim, B., and Chilkoti, A. 2004. Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion. Protein Sci. 13:3274-3284.
22. Urry, D.W. 1988. Entropic elastic processes in protein mechanisms. II. Simple (passive) and coupled (active) development of elastic forces. J. Protein Chem. 7:81-114.
23. Urry, D.W. 1993. Molecular machines: How motion and other functions of living organisms can result from reversible chemical changes. Angewandte Chemie International Edition in English 32:819-841.
24. Urry,D.W., Gowda, D.C., Parker, T.M., Luan, C.H., Reid, M.C., Harris, C.M., Pattanaik, A., and Harris, R.D. 1992. Hydrophobicity scale for proteins based on inverse temperature transitions. Biopolymers 32:1243-1250.
25. Urry, D.W., Trapane, T.L., and Prasad, K.U. 1985. Phase-structure transitions of the elastin polypentapeptide-water system within the framework of composition-temperature studies. Biopolymers 24:2345-2356.
26. Wood, D.W., Derbyshire,V.,Wu,W., Chartrain, M., Belfort, M., and Belfort, G. 2000. Optimized single-step affinity purification with a selfcleaving intein applied to human acidic fibroblast growth factor. Biotechnol. Prog. 16:1055-1063.
27. Wood, D.W., Wu, W., Belfort, G., Derbyshire, V., and Belfort, M. 1999. A genetic system yields self-cleaving inteins for bioseparations. Nat. Biotechnol. 17:889-892.