Host cell protein dynamics in recombinant CHO cells: Impacts from harvest to purification and beyond

Bioengineered

Volumn 4, Issue 5, 2013, Pages

  Hogwood, Catherine E M ^a   Bracewell, Daniel G ^b   Mark Smales, C ^a  

^a UNIVERSITY OF KENT   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Chinese hamster ovary (CHO); Downstream processing; Host cell protein; Mammalian cell culture; Monoclonal antibody; Protein a chromatography; Proteomics

Indexed keywords

CELL PROTEIN; RECOMBINANT PROTEIN; IMMUNOGLOBULIN FC FRAGMENT; MONOCLONAL ANTIBODY; STAPHYLOCOCCUS PROTEIN A;

CHO CELL; DOWNSTREAM PROCESSING; ENZYME LINKED IMMUNOSORBENT ASSAY; FERMENTATION; INFRARED SPECTROSCOPY; NONHUMAN; NOTE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN PURIFICATION; SURFACE ENHANCED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; AFFINITY CHROMATOGRAPHY; ANIMAL; BIOSYNTHESIS; CHO CELL LINE; CRICETULUS; DRUG CONTAMINATION; GENETIC ENGINEERING; GENETICS; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PREVENTION AND CONTROL; PROCEDURES; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, LIQUID; CRICETULUS; DRUG CONTAMINATION; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; GENETIC ENGINEERING; IMMUNOGLOBULIN FC FRAGMENTS; MASS SPECTROMETRY; RECOMBINANT PROTEINS; STAPHYLOCOCCAL PROTEIN A;

EID: 84883482792     PISSN: 19491018     EISSN: 19491026     Source Type: Journal    
DOI: None     Document Type: Note

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