Anion exchange membrane adsorbers for flow-through polishing steps: Part II. Virus, host cell protein, DNA clearance, and antibody recovery

Biotechnology and Bioengineering

Volumn 110, Issue 2, 2013, Pages 500-510

  Weaver, Justin ^a   Husson, Scott M ^b   Murphy, Louise ^a   Wickramasinghe, S Ranil ^a,c  

^a Engineering Research Center and Hydraulics Laboratory   (United States)

^b AMRL   (United States)

^c 3202 Bell Engineering Center   (United States)

Author keywords

Biopharmaceuticals; Bioseparations; Membrane chromatography; Monoclonal antibody; Protein purification; Purification

Indexed keywords

ADSORBERS; ANION EXCHANGE; ANION EXCHANGE MEMBRANE; BIO SEPARATION; BIOPHARMACEUTICALS; CHARGED CONTAMINANTS; CONTAMINANT REMOVAL; COULOMBIC INTERACTIONS; DNA CLEARANCE; FLOWTHROUGH; GUIDE SELECTION; HIGH CAPACITY; HIGH IONIC STRENGTH; HOST CELL PROTEIN; HYDROGEN BONDING INTERACTIONS; HYDROGEN PHOSPHATES; MEMBRANE ABSORBERS; MEMBRANE CHROMATOGRAPHY; POLISHING STEPS; PRIMARY AMINES; PROTEIN PURIFICATION; QUATERNARY AMINES; SECONDARY INTERACTIONS; SOLUTION CONDITIONS;

CONTAMINATION; HYDROGEN; HYDROGEN BONDS; IMPURITIES; IONIC STRENGTH; IONS; MOLECULAR BIOLOGY; MONOCLONAL ANTIBODIES; ORGANIC COMPOUNDS; POLISHING; PROTEINS; PURIFICATION; SECONDARY RECOVERY; SODIUM CHLORIDE; VIRUSES;

LIGANDS;

CELL PROTEIN; DNA; HOST CELL PROTEIN; MONOCLONAL ANTIBODY; PHOSPHATE; SODIUM CHLORIDE; UNCLASSIFIED DRUG;

ANION EXCHANGE; ANTIBODY RECOVERY; ARTICLE; CHEMICAL INTERACTION; DNA CLEARANCE; FLOW RATE; HOST CELL; HYDROGEN BOND; IONIC STRENGTH; MEASUREMENT; NONHUMAN; POLYMERASE CHAIN REACTION;

ADSORPTION; ANIMALS; ANION EXCHANGE RESINS; ANTIBODIES, MONOCLONAL; CHO CELLS; CHROMATOGRAPHY, ION EXCHANGE; CRICETINAE; CRICETULUS; DNA; DRUG CONTAMINATION; HYDROGEN-ION CONCENTRATION; MEMBRANES, ARTIFICIAL; MINUTE VIRUS OF MICE; PROTEINS; SODIUM CHLORIDE;

MINUTE VIRUS OF MICE;

EID: 84871722586     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24724     Document Type: Article

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