Expression, purification, and immunogenic characterization of Epstein-Barr virus recombinant EBNA1 protein in Pichia pastoris

Applied Microbiology and Biotechnology

Volumn 97, Issue 14, 2013, Pages 6251-6262

  Wang, Man ^a   Jiang, Shuai ^a   Liu, Xiaoying ^a   Wang, Yefu ^a  

^a WUHAN UNIVERSITY   (China)

Author keywords

EBNA1; Epstein Barr virus (EBV); Immunogenicity; Pichia pastoris; Polyclonal antibodies; Vaccine

Indexed keywords

EBNA1; EPSTEIN-BARR VIRUS; IMMUNOGENICITY; PICHIA PASTORIS; POLYCLONAL ANTIBODY;

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; ANTIGENS; IMMUNIZATION; MAMMALS; PURIFICATION; T-CELLS; VACCINES; VIRUSES; YEAST;

RECOMBINANT PROTEINS;

EPSTEIN BARR VIRUS ANTIGEN 1; POLYCLONAL ANTIBODY; POLYHISTIDINE TAG; SECRETORY PROTEIN; SUBUNIT VACCINE;

CHROMATOGRAPHY; GENE EXPRESSION; IMMUNE SYSTEM; PHYSIOLOGICAL RESPONSE; PROTEIN; PURIFICATION; RECOMBINATION; RODENT; TUMOR; VACCINATION; VIRUS; YEAST;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL LYSATE; CELL PROLIFERATION; CELLULAR IMMUNITY; CODON USAGE; CONTROLLED STUDY; CYTOKINE PRODUCTION; CYTOKINE RELEASE; EPSTEIN BARR VIRUS; FEMALE; HUMORAL IMMUNITY; IMMUNOGENICITY; LYMPHOCYTE PROLIFERATION; MOLECULAR WEIGHT; MOUSE; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SPLEEN CELL; STOP CODON; TH1 CELL; VIRUS RECOMBINANT; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, VIRAL; CYTOKINES; EPSTEIN-BARR VIRUS INFECTIONS; EPSTEIN-BARR VIRUS NUCLEAR ANTIGENS; FEMALE; GENE EXPRESSION; HERPESVIRUS 4, HUMAN; HUMANS; IMMUNIZATION; MICE; MICE, INBRED BALB C; PICHIA; RECOMBINANT PROTEINS; TH1 CELLS;

HERPESVIRIDAE; HUMAN HERPESVIRUS 4; MUS; PICHIA PASTORIS;

EID: 84879838566     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-4967-x     Document Type: Article

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