Efficient display of active Geotrichum sp. lipase on Pichia pastoris Cell wall and its application as a whole-cell biocatalyst to enrich EPA and DHA in fish oil

Journal of Agricultural and Food Chemistry

Volumn 60, Issue 38, 2012, Pages 9673-9679

  Pan, Xiao Xing ^a   Xu, Li ^a   Zhang, Yan ^a   Xiao, Xiao ^a   Wang, Xiao Feng ^a   Liu, Yun ^a   Zhang, Hou Jin ^a   Yan, Yun Jun ^a  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Geotrichum sp. lipase; Pichia pastoris; polyunsaturated fatty acid; surface display; whole cell biocatalyst

Indexed keywords

CELL WALLS; DOCOSAHEXAENOIC ACID; DRY CELLS; EICOSAPENTAENOIC ACID; FISH OIL; FLUORESCENCE ACTIVATED CELL SORTING; GEOTRICHUM; HIGH STABILITY; LIPASE ACTIVITY; OLIVE OIL; PICHIA PASTORIS; SURFACE DISPLAYS; TOTAL YIELD; WESTERN BLOTTING; WHOLE CELL; WHOLE CELL BIOCATALYSTS;

CELLS; FLUORESCENCE; POLYUNSATURATED FATTY ACIDS; VEGETABLE OILS; YEAST;

CELL CULTURE;

AGGLUTININ A; AGGLUTININ-A; DOCOSAHEXAENOIC ACID; FISH OIL; ICOSAPENTAENOIC ACID; IMMOBILIZED ENZYME; LECTIN; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE;

ARTICLE; CATALYSIS; CELL WALL; CHEMISTRY; DIET SUPPLEMENTATION; ENZYME STABILITY; ENZYMOLOGY; FLOW CYTOMETRY; GENETICS; GEOTRICHUM; HYDROLYSIS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PH; PICHIA; SACCHAROMYCES CEREVISIAE; TEMPERATURE;

BASE SEQUENCE; CATALYSIS; CELL WALL; DOCOSAHEXAENOIC ACIDS; EICOSAPENTAENOIC ACID; ENZYME STABILITY; ENZYMES, IMMOBILIZED; FISH OILS; FLOW CYTOMETRY; FOOD, FORTIFIED; GEOTRICHUM; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; LECTINS; LIPASE; MOLECULAR SEQUENCE DATA; PICHIA; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; TEMPERATURE;

GEOTRICHUM; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 84866700681     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf301827y     Document Type: Article

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