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Volumn 160, Issue 5, 2010, Pages 1321-1331
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Improvement of Aspergillus sulphureus endo-β-1,4-xylanase expression in Pichia pastoris by Codon optimization and analysis of the enzymic characterization
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Author keywords
Aspergillus sulphureus; Codon optimization; De novo synthesis; Endo 1,4 xylanase; Pichia pastoris
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Indexed keywords
BIOCHEMICAL CHARACTERISTICS;
CODON BIAS;
CODON OPTIMIZATION;
DE NOVO SYNTHESIS;
EXPRESSION VECTORS;
FLASK CULTURE;
GENE PROMOTER;
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE;
LIQUID MEDIUM;
OPTIMAL ACTIVITY;
OVERLAP EXTENSION POLYMERASE CHAIN REACTIONS;
P. PASTORIS;
PICHIA PASTORIS;
PROSPECTIVE APPLICATIONS;
RESIDUAL ACTIVITY;
SYNTHETIC DNA;
TRANSFORMANTS;
WILD TYPES;
XYLANASE ACTIVITY;
XYLANASES;
DNA;
ENZYME ACTIVITY;
GENE ENCODING;
GENE EXPRESSION;
OPTIMIZATION;
POLYMERASE CHAIN REACTION;
SODIUM;
ZINC;
SYNTHESIS (CHEMICAL);
CITRIC ACID;
ENDO BETA 1,4 XYLANASE;
FUNGAL DNA;
GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE;
RECOMBINANT ENZYME;
SYNTHETIC DNA;
UNCLASSIFIED DRUG;
XYLAN ENDO 1,3 BETA XYLOSIDASE;
ZINC;
ARTICLE;
ASPERGILLUS;
ASPERGILLUS SULPHUREUS;
CODON;
CONTROLLED STUDY;
ELECTROTRANSFORMATION;
ENZYME ACTIVITY;
ENZYME ANALYSIS;
ENZYME SYNTHESIS;
EXPRESSION VECTOR;
FUNGAL GENE;
FUNGAL STRAIN;
LIQUID CULTURE;
NONHUMAN;
NUCLEOTIDE SEQUENCE;
PICHIA PASTORIS;
POLYMERASE CHAIN REACTION;
PROTEIN EXPRESSION;
RNA SPLICING;
WILD TYPE;
ASPERGILLUS;
BASE SEQUENCE;
CODON;
ELECTROPHORESIS, POLYACRYLAMIDE GEL;
ENDO-1,4-BETA XYLANASES;
ENZYME STABILITY;
HYDROGEN-ION CONCENTRATION;
MOLECULAR SEQUENCE DATA;
PICHIA;
RECOMBINANT PROTEINS;
SEQUENCE ALIGNMENT;
SEQUENCE ANALYSIS, DNA;
TEMPERATURE;
ASPERGILLUS SULPHUREUS;
PICHIA PASTORIS;
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EID: 77949282641
PISSN: 02732289
EISSN: None
Source Type: Journal
DOI: 10.1007/s12010-009-8621-0 Document Type: Article |
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Times cited : (29)
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References (30)
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