Optimization of the production of gurmarin, a sweet-taste-suppressing protein, secreted by the methylotrophic yeast Pichia pastoris

Applied Microbiology and Biotechnology

Volumn 96, Issue 5, 2012, Pages 1253-1263

  Sigoillot, Maud ^a   Brockhoff, Anne ^b   Lescop, Ewen ^c   Poirier, Nicolas ^a   Meyerhof, Wolfgang ^b   Briand, Loïc ^a  

^a CENTRE DES SCIENCES DU GOÛT ET DE L ALIMENTATION   (France)

^b GERMAN INSTITUTE OF HUMAN NUTRITION   (Germany)

^c INSTITUT DE CHIMIE DES SUBSTANCES NATURELLES   (France)

Author keywords

Gurmarin; Gymnema sylvestre; Pichia pastoris; Sweetness suppressing peptide; Taste receptor; Yeast

Indexed keywords

ALCOHOL OXIDASE PROMOTERS; EXPRESSION SYSTEM; FERMENTORS; FUNCTIONAL EXPRESSION; GLUTAMIC ACID RESIDUES; GURMARIN; GYMNEMA SYLVESTRE; HETEROLOGOUS EXPRESSION; HUMAN EMBRYONIC KIDNEYS; METHYLOTROPHIC YEASTS; N-TERMINALS; P. PASTORIS; PICHIA PASTORIS; PRODUCTION LEVEL; PURIFIED PROTEIN; SECONDARY AND TERTIARY STRUCTURES; SIGNAL PEPTIDE; SWEET TASTE; TASTE RECEPTORS; YEAST CULTURE;

AMINO ACIDS; CELL CULTURE; MAMMALS; MASS SPECTROMETRY; METHANOL; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PEPTIDES; PURIFICATION; SODIUM CHLORIDE;

YEAST;

FUNGAL PROTEIN; GURMARIN; OXIDOREDUCTASE; POLYPEPTIDE; RECOMBINANT PROTEIN; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

AMINO ACID; BIOCHEMISTRY; FERMENTATION; GENE EXPRESSION; GENETIC ENGINEERING; INHIBITION; MOLECULAR ANALYSIS; OPTIMIZATION; PEPTIDE; PROTEIN; RECOMBINATION; RODENT; SECRETION; TASTE; YEAST;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; CELL STRAIN HEK293; CIRCULAR DICHROISM; CONTROLLED STUDY; EMBRYO; FUNGUS CULTURE; HETEROLOGOUS EXPRESSION; HUMAN; HUMAN CELL; MASS SPECTROMETRY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PEPTIDE SYNTHESIS; PICHIA PASTORIS; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN SECRETION; PROTEIN TERTIARY STRUCTURE; RAT; SACCHAROMYCES CEREVISIAE; SWEETNESS; TASTE BUD;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CIRCULAR DICHROISM; GENE EXPRESSION; MAGNETIC RESONANCE SPECTROSCOPY; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PICHIA; PLANT PROTEINS; PROMOTER REGIONS, GENETIC; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN SORTING SIGNALS; RATS; RECEPTORS, G-PROTEIN-COUPLED; RECOMBINANT PROTEINS;

GYMNEMA SYLVESTRE; PICHIA PASTORIS; RATTUS; RODENTIA; SACCHAROMYCES CEREVISIAE;

EID: 84871032677     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-3897-3     Document Type: Article

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