Characterization of secreted recombinant Toxoplasma gondii surface antigen 2 (SAG2) heterologously expressed by the yeast Pichia pastoris

Biotechnology Letters

Volumn 30, Issue 4, 2008, Pages 611-618

  Fong, Mun Yik ^a   Lau, Yee Ling ^b   Zulqarnain, Mohamed ^c  

^a UNIVERSITY OF MALAYA   (Malaysia)

^b MONASH UNIVERSITY MALAYSIA   (Malaysia)

^c UNIVERSITY OF MALAYA   (Malaysia)

Author keywords

Pichia pastoris; Recombinant SAG2 antigen; Toxoplasma gondii

Indexed keywords

ANTIBODIES; BIOASSAY; CLONING; GENE EXPRESSION; PLASMA (HUMAN); YEAST;

PICHIA PASTORIS; RECOMBINANT SAG2 ANTIGENS; TOXOPLASMA ANTIBODY POSITIVE HUMAN SERUM; TOXOPLASMA GONDII;

ANTIGENS;

PARASITE ANTIGEN; PROTOZOAL PROTEIN; RECOMBINANT PROTEIN; SURFACE ANTIGEN P22, TOXOPLASMA;

ANIMAL; ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; GENETICS; IMMUNOLOGY; INSTITUTE FOR CANCER RESEARCH MOUSE; METABOLISM; MOUSE; PICHIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; TOXOPLASMA; WESTERN BLOTTING;

ANIMALS; ANTIGENS, PROTOZOAN; BLOTTING, WESTERN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; MICE; MICE, INBRED ICR; PICHIA; POLYMERASE CHAIN REACTION; PROTOZOAN PROTEINS; RECOMBINANT PROTEINS; TOXOPLASMA;

MUS; PICHIA PASTORIS; PROTOZOA; TOXOPLASMA; TOXOPLASMA GONDII;

EID: 43149113208     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-007-9609-x     Document Type: Article

References (12)

1. Beghetto E, Spadoni A, Bruno L, Buffolano W, Gargano N (2006) Chimeric antigens of Toxoplasma gondii: toward standardization of toxoplasmosis serodiagnosis using recombinant products. J Clin Microbiol 44:2133-2140
2. Biemans R, Grégoire D, Haumont M, Bosseloir A, Garcia L, Jacquet A, Dubeaux C, Bollen A (1998) The conformation of purified Toxoplasma gondii SAG1 antigen, secreted from engineered Pichia pastoris, is adequate for serorecognition and cell proliferation. J Biotechnol 66:137-146
3. Cereghino JL, Cregg JM (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24:45-66
4. Fong MY, Lau YL (2004) Recombinant expression of the larval excretory-secretory antigen TES-120 of Toxocara canis in the methylotrophic yeast Pichia pastoris. Parasitol Res 92:173-176
5. Hill D, Dubey JP (2002) Toxoplasma gondii: transmission, diagnosis and prevention. Clin Microbiol Infect 8:634-640
6. Kaplan EL, Meier P (1958) Nonparametric estimation from incomplete observations. J Am Stat Assoc 53:457-481
7. Letourneur O, Gervasi G, Gaia S, Pages J, Watelet B, Jolivet M (2001) Characterization of Toxoplasma gondii surface antigen 1 (SAG1) secreted from Pichia pastoris: evidence of hyper O-glycosylation. Biotechnol Appl Biochem 33:35-45
8. Mishima M, Xuan X, Shioda A, Omata Y, Fujisaki K, Nagasawa H, Mikami T (2001) Modified protection against Toxoplasma gondii lethal infection and brain cyst formation by vaccination with SAG2 and SRS1. J Vet Med Sci 63:433-438
9. Parmley SF, Sgarlato GD, Mark J, Prince JB, Remington JS (1992) Expression, characterization, and serologic reactivity of recombinant surface antigen P22 of Toxoplasma gondii. J Clin Microbiol 30:1127-1133
10. Pietkiewicz H, Hiszczyńska-Sawicka E, Kur J, Petersen E, Nielsen HV, Stankiewicz M, Andrzejewska I, Myjak P (2004) Usefulness of Toxoplasma gondii-specific recombinant antigens in serodiagnosis of human toxoplasmosis. J Clin Microbiol 42:1779-1781
11. Prince JB, Auer KL, Huskinson J, Parmley SF, Araujo FG, Remington JS (1990) Cloning, expression, and cDNA sequence of surface antigen P22 from Toxoplasma gondii. Mol Biochem Parasitol 43:97-106
12. Zhou H, Gu Q, Zhao Q, Zhang J, Cong H, Li Y, He S (2007) Toxoplasma gondii: expression and characterization of a recombinant protein containing SAG1 and GRA2 in Pichia pastoris. Parasitol Res 100:829-835