Differential response in downstream processing of CHO cells grown under mild hypothermic conditions

Biotechnology Progress

Volumn 29, Issue 3, 2013, Pages 688-696

  Tait, Andrew S ^a   Tarrant, Richard D R ^a   Velez Suberbie, M Lourdes ^a   Spencer, Daniel I R ^b   Bracewell, Daniel G ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b Culham Science Centre   (United Kingdom)

Author keywords

Apoptosis; Cell cycle; Downstream processing; Host cell proteins; Mild hypothermic culture

Indexed keywords

CELL CYCLE; DIFFERENTIAL RESPONSE; DOWNSTREAM-PROCESSING; HOST CELL PROTEIN; HYPOTHERMIC; HYPOTHERMIC CONDITIONS; PROCESS CHARACTERIZATION; VOLUMETRIC PRODUCTIVITY;

CELL CULTURE; CELL DEATH; PHYSIOLOGY; PRODUCTIVITY; PROTEINS;

MOLECULAR BIOLOGY;

PROTEIN;

ANIMAL; APOPTOSIS; ARTICLE; BIOMECHANICS; CELL SIZE; CELL SURVIVAL; CENTRIFUGATION; CHEMISTRY; CHO CELL; COLD; CRICETULUS; CULTURE TECHNIQUE; GLYCOSYLATION; HAMSTER; MASS SPECTROMETRY; MECHANICAL STRESS; METABOLISM; METHODOLOGY; PHYSIOLOGY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ANIMALS; APOPTOSIS; BIOMECHANICAL PHENOMENA; CELL CULTURE TECHNIQUES; CELL SIZE; CELL SURVIVAL; CENTRIFUGATION; CHO CELLS; COLD TEMPERATURE; CRICETINAE; CRICETULUS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; GLYCOSYLATION; MASS SPECTROMETRY; PROTEINS; STRESS, MECHANICAL;

MAMMALIA;

EID: 84879270705     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.1726     Document Type: Article

References (40)

1. Aldington S, Bonnerjea J. Scale-up of monoclonal antibody purification processes. J Chromatogr B. 2007;848:64-78.
2. Kelley B. Very large scale monoclonal antibody purification: the case for conventional unit operations. Biotechnol Prog. 2007;23:995-1008.
3. Al-Fageeh MB, Marchant RJ, Carden MJ, Smales CM. The cold-shock response in cultured mammalian cells: harnessing the response for the improvement of recombinant protein production. Biotechnol Bioeng. 2006;93:829-835.
4. Trummer E, Fauland K, Seidinger S, Schriebl K, Lattenmayer C, Kunert R, Vorauer-Uhl K, Weik R, Borth N, Katinger H, Muller D. Process parameter shifting: Part I. Effect of DOT, pH, and temperature on the performance of Epo-Fc expressing CHO cells cultivated in controlled batch bioreactors. Biotechnol Bioeng. 2006;94:1033-1044.
5. Tharmalingam T, Sunley K, Butler M. High yields of monomeric recombinant beta-interferon from macroporous microcarrier cultures under hypothermic conditions. Biotechnol Prog. 2008;24:832-838.
6. Kaufmann H, Mazur X, Fussenegger M, Bailey JE. Influence of low temperature on productivity, proteome and protein phosphorylation of CHO cells. Biotechnol Bioeng. 1999;63:573-582.
7. Ahn WS, Jeon JJ, Jeong YR, Lee SJ, Yoon SK. Effect of culture temperature on erythropoietin production and glycosylation in a perfusion culture of recombinant CHO cells. Biotechnol Bioeng. 2008;101:1234-1244.
8. Sunley K, Butler M. Strategies for the enhancement of recombinant protein production from mammalian cells by growth arrest. Biotechnol Adv. 2010;28:385-394.
9. Chuppa S, Tsai YS, Yoon S, Shackleford S, Rozales C, Bhat R, Tsay G, Matanguihan C, Konstantinov K, Naveh D. Fermentor temperature as a tool for control of high-density perfusion cultures of mammalian cells. Biotechnol Bioeng. 1997;55:328-338.
10. Kumar N, Gammell P, Clynes M. Proliferation control strategies to improve productivity and survival during CHO based production culture: a summary of recent methods employed and the effects of proliferation control in product secreting CHO cell lines. Cytotechnology. 2007;53:33-46.
11. Bi JX, Shuttleworth J, Al-Rubeai M. Uncoupling of cell growth and proliferation results in enhancement of productivity in p21CIP1-arrested CHO cells. Biotechnol Bioeng. 2004;85:741-749.
12. Marchant RJ, Al-Fageeh MB, Underhill MF, Racher AJ, Smales, CM. Metabolic rates, growth phase, and mRNA levels influence cell-specific antibody production levels from in vitro-cultured mammalian cells at sub-physiological temperatures. Mol Biotechnol. 2008;39:69-77.
13. Sakurai T, Itoh K, Liu Y, Higashitsuji H, Sumitomo Y, Sakamaki K, Fujita J. Low temperature protects mammalian cells from apoptosis initiated by various stimuli in vitro. Exp Cell Res. 2005;309:264-272.
14. Yoon SK, Hong JK, Choo SH, Song JY, Park HW, Lee GM. Adaptation of Chinese hamster ovary cells to low culture temperature: cell growth and recombinant protein production. J Biotechnol. 2006;122:463-472.
15. Hossler P, Khattak SF, Li ZJ. Optimal and consistent protein glycosylation in mammalian cell culture. Glycobiology. 2009;19:936-949.
16. Kessler M, Goldsmith D, Schellekens H. Immunogenicity of biopharmaceuticals. Nephrol Dial Transpl. 2006;21 Suppl 5:v9-12.
17. Rathore AS, Winkle H. Quality by design for biopharmaceuticals. Nat Biotechnol. 2009;27:26-34.
18. Bareither R, Pollard D. A review of advanced small-scale parallel bioreactor technology for accelerated process development: current state and future need. Biotechnology Progr. 2011;27:2-14.
19. Boychyn M, Yim SSS, Ayazi Shamlou P, Bulmer M, More J, Hoare M. Characterization of flow intensity in continuous centrifuges for the development of laboratory mimics. Chem Eng Sci. 2001;56:4759-4770.
20. Westoby M, Rogers JK, Haverstock R, Romero J, Pieracci J. Modeling industrial centrifugation of mammalian cell culture using a capillary based scale-down system. Biotechnol Bioeng. 2011;108:989-998.
21. Jackson NB, Liddell JM, Lye GJ. An automated microscale technique for the quantitative and parallel analysis of microfiltration operations. J Membr Sci. 2006;276:31-41.
22. Hogwood CE, Tait AS, Koloteva-Levine N, Bracewell DG, Smales CM. The dynamics of the CHO host cell protein profile during clarification and protein A capture in a platform antibody purification process. Biotechnol Bioeng. 2012;110:240-251.
23. Tait AS, Aucamp JP, Bugeon A, Hoare M. Ultra scale-down prediction using microwell technology of the industrial scale clarification characteristics by centrifugation of mammalian cell broths. Biotechnol Bioeng. 2009;104:321-331.
24. Tait AS, Hogwood CE, Smales CM, Bracewell DG. Host cell protein dynamics in the supernatant of a mAb producing CHO cell line. Biotechnol Bioeng. 2012;109:971-982.
25. Velez-Suberbie ML, Tarrant RD, Tait AS, Spencer DI, Bracewell DG. Impact of aeration strategy on CHO cell performance during antibody production. Biotechnol Prog. 2013;29:116-126.
26. Tarrant RD, Velez-Suberbie ML, Tait AS, Smales CM, Bracewell DG. Host cell protein adsorption characteristics during protein a chromatography. Biotechnol Prog. 2012;28:1037-1044.
27. Yoon SK, Choi SL, Song JY, Lee GM. Effect of culture pH on erythropoietin production by Chinese hamster ovary cells grown in suspension at 32.5 and 37.0°C. Biotechnol Bioeng. 2005;89:345-356.
28. Reid CQ, Tait A, Baldascini H, Mohindra A, Racher A, Bilsborough S, Smales CM, Hoare M. Rapid whole monoclonal antibody analysis by mass spectrometry: an ultra scale-down study of the effect of harvesting by centrifugation on the post-translational modification profile. Biotechnol Bioeng. 2010;107:85-95.
29. Jin M, Szapiel N, Zhang J, Hickey J, Ghose S. Profiling of host cell proteins by two-dimensional difference gel electrophoresis (2D-DIGE): implications for downstream process development. Biotechnol Bioeng. 2009;105:306-316.
30. Flatman S, Alam I, Gerard J, Mussa N. Process analytics for purification of monoclonal antibodies. J Chromatogr B. 2007;848:79-87.
31. Smales CM, Dinnis DM, Stansfield SH, Alete D, Sage EA, Birch JR, Racher AJ, Marshall CT, James DC. Comparative proteomic analysis of GS-NS0 murine myeloma cell lines with varying recombinant monoclonal antibody production rate. Biotechnol Bioeng. 2004;88:474-488.
32. Kumar N, Maurya P, Gammell P, Dowling P, Clynes M, Meleady P. Proteomic profiling of secreted proteins from CHO cells using Surface-Enhanced Laser desorption ionization time-of-flight mass spectrometry. Biotechnol Prog. 2008;24:273-278.
33. Wang X, Hunter AK, Mozier NM. Host cell proteins in biologics development: identification, quantitation and risk assessment. Biotechnol Bioeng. 2009;103:446-458.
34. Godoy-Silva R, Chalmers JJ, Casnocha SA, Bass LA, Ma N. Physiological responses of CHO cells to repetitive hydrodynamic stress. Biotechnol Bioeng. 2009;103:1103-1117.
35. Al-Rubeai M, Singh RP, Emery AN, Zhang Z. Cell cycle and cell size dependence of susceptibility to hydrodynamic forces. Biotechnol Bioeng. 1995;46:88-92.
36. Boychyn M, Yim SS, Bulmer M, More J, Bracewell DG, Hoare M. Performance prediction of industrial centrifuges using scale-down models. Bioproc Biosyst Eng. 2004;26:385-391.
37. Hutchinson N, Bingham N, Murrell N, Farid S, Hoare M. Shear stress analysis of mammalian cell suspensions for prediction of industrial centrifugation and its verification. Biotechnol Bioeng. 2006;95:483-491.
38. Ramirez OT, Mutharasan R. The role of the plasma membrane fluidity on the shear sensitivity of hybridomas grown under hydrodynamic stress. Biotechnol Bioeng. 1990;36:911-920.
39. Roobol A, Roobol J, Carden MJ, Bastide A, Willis AE, Dunn WB, Goodacre R, Smales CM. ATR (ataxia telangiectasia mutated- and Rad3-related kinase) is activated by mild hypothermia in mammalian cells and subsequently activates p53. Biochem J. 2011;435:499-508.
40. Los DA, Murata N. Membrane fluidity and its roles in the perception of environmental signals. BBA-Rev Biomembr. 2004;1666:142-157.