Effect of culture temperature on erythropoietin production and glycosylation in a perfusion culture of recombinant CHO cells

Biotechnology and Bioengineering

Volumn 101, Issue 6, 2008, Pages 1234-1244

  Woo, Suk Ahn ^a   Jeon, Jae Jin ^a   Jeong, Yeong Ran ^a   Seung, Joo Lee ^a   Sung, Kwan Yoon ^a,b  

^a LG Chemical Ltd   (South Korea)

^b Aprogen   (South Korea)

Author keywords

CHO cells; Erythropoietin; Glycosylation; Low culture temperature; Perfusion; Specific EPO productivity

Indexed keywords

ACIDS; CELL GROWTH; CELLS; CHROMATOGRAPHIC ANALYSIS; CONCENTRATION (PROCESS); CYTOLOGY; ELECTROPHORESIS; ESTERIFICATION; GLYCOSYLATION; GROWTH KINETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

CHO CELLS; ERYTHROPOIETIN; LOW CULTURE TEMPERATURE; PERFUSION; SPECIFIC EPO PRODUCTIVITY;

CELL CULTURE;

RECOMBINANT ERYTHROPOIETIN;

ANIMAL CELL; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CELL CULTURE; CELL VIABILITY; CHO CELL; CONTROLLED STUDY; DRUG QUALITY; DRUG SYNTHESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOELECTRIC FOCUSING; NONHUMAN; PROTEIN GLYCOSYLATION; RECOMBINANT DNA TECHNOLOGY; TEMPERATURE DEPENDENCE;

ANIMALS; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; CRICETINAE; CRICETULUS; ERYTHROPOIETIN; GLYCOSYLATION; HUMANS; ISOELECTRIC FOCUSING; RECOMBINANT PROTEINS; TEMPERATURE; TIME FACTORS;

CRICETULUS GRISEUS; TETRA;

EID: 56749142714     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22006     Document Type: Article

References (33)

1. Anderson DC, Goochee CF. 1995. The effect of ammonia on the O-linked glycosylation of granulocyte colony-stimulating factor produced by Chinese hamster ovary cells. Biotechnol Bioeng 47:96-105.
2. Anderson DC, Bridges T, Gawlitzek M, Hoy C. 2000. Multiple cell culture factors can affect the glycosylation of Asn-184 in CHO-produced tissue-type plasminogen activator. Biotechnol Bioeng 70:25-31.
3. Borys MC, Linzer DIH, Papoutsakis ET. 1993. Culture pH affects expression rates and glycosylation of recombinant mouse placental lactogen proteins by Chinese hamster ovary (CHO) cells. Bio/Technology 11:720-724.
4. Chang KH. 2000. Effect of thiol reducing agents on production and glycosylation of recombinant erythropoietin. PhD thesis, Korea Advanced Institute of Science and Technology, Daejon, Korea.
5. Chen Z-L, Wu B-C, Liu H, Liu X-M, Huang P-T. 2004. Temperature shift as a process optimization step for the production of pro-urokinase by a recombinant Chinese hamster ovary cell line in high-density perfusion culture. J Biosci Bioeng 97:239-243.
6. Chuppa S, Tsai Y-S, Yoon S, Shackleford S, Rozales C, Bhat R, Tsay G, Matanguihan C, Konstantinov K, Naveh D. 1997. Fermentor temperature as a tool for control of high-density perfusion cultures of mammalian cells. Biotechnol Bioeng 55:328-338.
7. Dube S, Fisher JW, Powell JS. 1988. Glycosylation at specific sites of erythropoietin is essential for biosynthesis, secretion, and biological function. J Biol Chem 25:17516-17521.
8. Fogolín MB, Wagner R, Etcheverrigaray M, Kratje R. 2004. Impact of temperature reduction and expression of yeast pyruvate carboxylase on hGM-CSF-producing CHO cells. J Biotechnol 109:179-191.
9. Fogolín MB, Forno G, Nimtz M, Conradt HS, Etcheverigaray M, Kratje R. 2005. Temperature reduction in cultures of hGM-CSF-expressing CHO cells: Effect of productivity and product quality. Biotechnol Prog 21:17-21.
10. Fox SR, Patel UA, Yap MGS, Wang DIC. 2004. Maximizing interferon-γ production by Chinese hamster ovary cells through temperature shift optimization: Experimental and modeling. Biotechnol Bioeng 85:177-184.
11. Fukuda MN, Sasaki H, Lopez L, Fukuda M. 1989. Survival of recombinant erythropoietin in the circulation: The role of carbohydrates. Blood 73:84-89.
12. Furukawa K, Ohsuye K. 1998. Effect of culture temperature on a recombinant CHO cell line producing a C-terminal α-amidating enzyme. Cytotechnology 26:153-164.
13. Gramer MJ, Goochee CF. 1993. Glycosidase activities in Chinese hamster ovary cell lysate and cell culture supernatant. Biotechnol Prog 9:366-373.
14. Kaufmann H, Mazur X, Marone R, Bailey JE, Fussenegger M. 2001. Comparative analysis of two controlled proliferation strategies regarding product quality, influence on tetracycline-regulated gene expression and productivity. Biotechnol Bioeng 72:592-602.
15. Ludwig A, Tomeczkowski J, Kretzmer G. 1992. Influence of the temperature on the shear stress sensitivity of adherent BHK 21 cells. Appl Microbiol Biotechnol 38:323-327.
16. Maiorella BL, Winkelhake J, Young J, Moyer B, Bauer R, Hora M, Andya J, Thomson J, Patel T, Parekh R. 1993. Effect of culture conditions on IgM antibody structure, pharmacokinetics and activity. Bio/Technology 11:387-392.
17. Munzert E, Müthing J, Büntemeyer H, Lehmann J. 1996. Sialidase activity in culture fluid of Chinese hamster ovary cells during batch culture and its effect on recombinant human antithrombin III integrity. Biotechnol Prog 12:559-563.
18. Nimtz M, Wray V, Rüdiger A, Conradt HS. 1995. Identification and structural characterization of a mannose-6-phosphate containing oligomannosidic N-glycan from human erythropoietin secreted by recombinant BHK-21 cells. FEBS Lett 365:203-208.
19. Restelli V, Wang M-D, Huzel N, Ethier M, Perreault H, Butler M. 2006. The effect of dissolved oxygen on the production and the glycosylation profile of recombinant human erythropoietin produced from CHO cells. Biotechnol Bioeng 94:481-494.
20. Schatz SM, Kerschbaumer RJ, Gerstenbauer G, Kral M, Dorner F, Scheiflinger F. 2003. Higher expression of Fab antibody fragments in a CHU cell line at reduced temperature. Biotechnol Bioeng 84:433-438.
21. Takeuchi M, Inoue N, Strickland TW, Kubota M, Wada M, Shimizu R, Hoshi S, Kozutsumi H, Takasaki S, Kobata A. 1989. Relationship between sugar chain structure and biological activity of recombinant human erythropoietin produced in Chinese hamster ovary cells. Proc Natl Acad Sci USA 86:7819-7822.
22. Trummer E, Fauland K, Seidinger S, Schriebl K, Lattenmayer C, Kunert R, Vorauer-Uhl K, Weik R, Borth N, Katinger H, Müller D. 2006a. Process parameter shifting: Part I. Effect of DOT, pH and temperature on the performance of EPO-Fc expressing CHO cells cultivated in controlled batch bioreactors. Biotechnol Bioeng 94:1033-1044.
23. Trummer E, Fauland K, Seidinger S, Schriebl K, Lattenmayer C, Kunert R, Vorauer-Uhl K, Weik R, Borth N, Katinger H, Müller D. 2006b. Process parameter shifting: Part II. Biphasic cultivation-A tool for enhancing the volumetric productivity of batch processes using EPO-Fc expressing CHO cells. Biotechnol Bioeng 94:1045-1052.
24. Watson E, Shah B, Leiderman L, Hsu Y-R, Karkare S, Lu HS, Lin F-K. 1994. Comparison of N-linked oligosaccharides of recombinant human tissue kallikrein produced by Chinese hamster ovary cells on microcarrier beads and in serum-free suspension culture. Biotechnol Prog 10:39-44.
25. Yamaguchi K, Akai K, Kawanishi G, Ueda M, Masuda S, Sasaki R. 1991. Effect of site-directed removal of N-glycosylation sites in human erythropoietin on its production and biological properties. J Biol Chem 266:20434-20439.
26. Yang M, Butler M. 2000a. Effect of ammonia on CHO cell growth, erythropoietin production and glycosylation. Biotechnol Bioeng 68:370-380.
27. Yang M, Butler M. 2000b. Effect of ammonia on the glycosylation of human recombinant erythropoietin in culture. Biotechnol Prog 16:751-759.
28. Yoon SK, Song JY, Lee GM. 2003a. Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese hamster ovary cells. Biotechnol Bioeng 82:289-298.
29. Yoon SK, Kim SH, Lee GM. 2003b. Effect of low culture temperature on specific productivity and transcription level of anti-4-1BB antibody in recombinant Chinese hamster ovary cells. Biotechnol Prog 19:1383-1386.
30. Yoon SK, Hong JK, Lee GM. 2004a. Effect of simultaneous application of stressful culture conditions on specific productivity and heterogeneity of erythropoietin in Chinese hamster ovary cells. Biotechnol Prog 20:1293-1296.
31. Yoon SK, Hwang SO, Lee GM. 2004b. Enhancing effect of low culture temperature on specific antibody productivity of recombinant Chinese hamster ovary cells: Clonal variation. Biotechnol Prog 20:1683-1688.
32. Yoon SK, Ahn Y-H, Jeong MH. 2007. Effect of culture temperature on follicle-stimulating hormone production by Chinese hamster ovary cells in a perfusion bioreactor. Appl Microbiol Biotechnol 76:83-89.
33. Yuen C-T, Storring PL, Tiplady RJ, Izquierdo M, Wait R, Gee CH, Gerson P, Lloyd P, Cremata JA. 2003. Relationship between the N-glycan structures and biological activities of recombinant human erythropoietins produced using different culture conditions and purification procedures. Br J Haematology 121:511-526.