High yields of monomeric recombinant β-interferon from macroporous microcarrier cultures under Hypothermic Conditions

Biotechnology Progress

Volumn 24, Issue 4, 2008, Pages 832-838

  Tharmalingam, Tharmala ^a   Sunley, Kevin ^a   Butler, Michael ^a  

^a UNIVERSITY OF MANITOBA   (Canada)

Author keywords

Bioreactor; Biphasic; CHO; Cytopore; Hypothermic; Interferon ; Low temperature; Microcarrier

Indexed keywords

AGGLOMERATION; ANIMAL CELL CULTURE; ANTIBIOTICS; CELL GROWTH; CELLS; CYTOLOGY; GLYCOPROTEINS; GROWTH KINETICS; LOW TEMPERATURE PRODUCTION; MAMMALS; OSTEOBLASTS; SUSPENSIONS (COMPONENTS);

BIOREACTOR; BIPHASIC; CELL CONCENTRATIONS; CHINESE HAMSTER OVARY CELLS; CHO; CONTROL CULTURES; CULTURE CONDITIONS; CYTOPORE; CYTOPORE MICROCARRIERS; GROWTH RATES; HYPOTHERMIC; HYPOTHERMIC CONDITIONS; INTERFERON (; INTRAMOLECULAR AGGREGATION; LOW TEMPERATURE; LOW-TEMPERATURE REGIME; MACROPOROUS; MAMMALIAN CELLS; MESH NETWORKS; MICROCARRIER; MICROCARRIERS; PROTEIN PRODUCTIONS; RECOMBINANT PROTEINS; SUSPENSION CULTURES; SYNERGISTIC EFFECTS; VOLUMETRIC PRODUCTION;

CELL CULTURE;

BETA INTERFERON; RECOMBINANT PROTEIN;

ANIMAL; BIOREACTOR; BIOTECHNOLOGY; CELL PROLIFERATION; CHO CELL; COLD; CONFERENCE PAPER; CRICETULUS; CULTURE TECHNIQUE; EVALUATION; GENETICS; HAMSTER; HUMAN; METABOLISM; METHODOLOGY;

ANIMALS; BIOREACTORS; BIOTECHNOLOGY; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CHO CELLS; COLD TEMPERATURE; CRICETINAE; CRICETULUS; HUMANS; INTERFERON-BETA; RECOMBINANT PROTEINS;

CRICETULUS GRISEUS; MAMMALIA;

EID: 51849161375     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1002/btpr.8     Document Type: Conference Paper

References (45)

1. Chu L, Robinson DK. Industrial choices for protein production by large-scale cell culture. Curr Opin Biotechnol. 2001;12:180-187.
2. Sethuraman N, Stadheim TA. Challenges in therapeutic glycoprotein production. Curr Opin Biotechnol. 2006;17:341-346.
3. Jenkins N, Parekh RB, James DC. Getting the glycosylation right: implications for the biotechnology industry. Nat Biotechnol. 1996;14:975-981.
4. Andersen DC, Krummen L. Recombinant protein expression for therapeutic applications. Curr Opin Biotechnol. 2002;13:117-123.
5. Arnason BG. Immunologic therapy of multiple sclerosis. Annu Rev Med. 1999;50:291-302.
6. Chofflon M. Multiple sclerosis: current therapeutic strategies. Rev Med Suisse Romande. 2003;123:27-32.
7. Filippini G, Munari L, Incorvaia B, Ebers GC, Polman C, D'Amico R, Rice GPA. Interferons in relapsing remitting multiple sclerosis: a systematic review. Lancet. 2003;361:545-552.
8. Arduini RM, Li Z, Rapoza A, Gronke R, Hess DM, Wen D, Miatkowski K, Coots C, Kaffashan A, Viseux N. Expression, purification, and characterization of rat interferon-β, and preparation of an N-terminally PEGylated form with improved pharmacokinetic parameters. Protein Expr Purif. 2004;34:229-242.
9. Lin L, Betaseron. Dev Biol Stand. 1998;96:97-104.
10. Runkel L, Meier W, Pepinsky RB, Karpusas M, Whitty A, Kimball K, Brickelmaier M, Muldowney C, Jones W, Goelz SE. Structural and functional differences between glycosylated and non-glycosylated forms of human interferon-β (IFN-β). Pharm Res. 1998;15:641-649.
11. Spearman M, Rodriguez J, Huzel N, Butler M. Production and glycosylation of recombinant β-interferon in suspension and cytopore microcarrier cultures of CHO cells. Biotechnol Prog. 2005;21:31-39.
12. Kagawa Y, Takasaki S, Utsumi J, Hosoi K, Shimizu H, Kochibe N, Kobata A. Comparative study of the asparagine-linked sugar chains of natural human interferon-β 1 and recombinant human interferon-β 1 produced by three different mammalian cells. J Biol Chem. 1988;263:17508-17515.
13. Karpusas M, Whitty A, Runkel L, Hochman P. The structure of human interferon-β: implications for activity. Cell Mol Life Sci. 1998;54:1203-1216.
14. Rodriguez J, Spearman M, Huzel N, Butler M. Enhanced production of monomeric interferon-β by CHO cells through the control of culture conditions. Biotechnol Prog. 2005;21:22-30.
15. Berry JM, Barnabe N, Coombs KM, Butler M. Production of Reovirus Type-1 and Type-3 from Vero cells grown on solid and macroporous microcarriers. Biotechnol Bioeng. 1999;62:12-19.
16. Frazzati-Gallina NM, Paoli RL, Mourao-Fuches RM, Jorge SAC, Pereira CA. Higher production of rabies virus in serum-free medium cell cultures on microcarriers. J Biotechnol. 2001;92:67-72.
17. Sun M-B, Jiang Y-J, Li W-D, Li P-Z, Li G-L, Jiang S-D, Liao G-Y. A novel process for production of hepatitus A virus in Vero cells grown on microcarriers in bioreactor. World J Gasteroenterol. 2004;10:2571-2573.
18. Yokomizo AY, Antoniazzi MM, Galdino PL, Azambuja N, Jorge SAC, Pereira CA. Rabies virus production in high Vero cell density cultures on macroporous microcarriers. Biotechnol Bioeng. 2004;85:506-515.
19. Wu SC, Liu CC, Lian WC. Optimization of microcarrier cell culture process for the inactivated enterovirus type 71 vaccine development. Vaccine. 2004;22:3858-3864.
20. Butler M, Burgener A, Patrick M, Berry M, Moffatt D, Huzel N, Barnabe N, Coombs K. Application of a serum-free medium for the growth of Vero cells and the production of Reovirus. Biotechnol Prog. 2000;16:854-858.
21. Duerrschmid M, Landauer K, Simic G, Blueml G, Doblhoff-Dier O. Scalable inoculation strategies for microcarrier-based animal cell bioprocesses. Biotechnol Bioeng. 2003;83:681-686.
22. Wang MD, Yang M, Huzel N, Butler M. Erythropoietin production from CHO cells grown by continuous culture in a fluidized-bed bioreactor. Biotechnol Bioeng. 2002;77:194-203.
23. Kong D, Cardak S, Chen M, Gentz R, Zhang J. High cell density and productivity culture of Chinese hamster ovary cells in a fluidized bed bioreactor. Cytotechnology. 1999;29:215-220.
24. Ng YC, Berry JM, Butler M. Optimization of physical parameters for cell attachment and growth on macroporous microcarriers. Biotechnol Bioeng. 1996;50:627-635.
25. Mignot G, Faure T, Ganne V, Arbeille B, Pavirani A, Romet-Lemonne JL. Production of recombinant Von Willebrand factor by CHO cells cultured in macroporous microcarriers. Cytotechnology. 1990;4:163-171.
26. Chen Z, Lütkemeyer D, Iding K, Lehmann J. High-density culture of recombinant Chinese hamster ovary cells producing prothrombin in protein-free medium. Biotechnol Lett. 2001;23:767-770.
27. Xiao C, Huang Z, Li W, Hu X, Qu W, Gao L, Liu G. High density and scale-up cultivation of recombinant CHO cell line and hybridomas with porous microcarrier Cytopore. Cytotechnology. 1999;30:143-147.
28. Hu X, Xiao C, Huang Z, Guo Z, Zhang Z, Li Z. Pilot production of u-PA with porous microcarrier cell culture. Cytotechnology. 2000;33:13-19.
29. Zhaolie C, Benchuan W, Hong L, Shichong L, Peitang H. Study on rt-PA production by recombinant CHO cells immobilized with a porous microcarrier. Chin J Biotechnol. 1999;15:239-244.
30. Chen Z, Wu B, Liu H, Liu X, Huang P. Temperature shift as a process optimization step for the production of pro-urokinase by a recombinant Chinese hamster ovary cell line in high-density perfusion culture. J Biosci Bioeng. 2004;97:239-243.
31. Shi M, Xie Z, Yu M, Shen B, Guo N. Controlled growth of Chinese hamster ovary cells and high expression of antibody-IL-2 fusion proteins by temperature manipulation. Biotechnol Lett. 2005;27:1879-1884.
32. Fogolin MB, Wagner R, Etcheverrigaray M, Kratje R. Impact of temperature reduction and expression of yeast pyruvate carboxylase on hGM-CSF-producing CHO cells. J Biotechnol. 2004;109:179-191.
33. Yoon SK, Kim SH, Lee GM. Effect of low culture temperature on specific productivity and transcription level of anti-4-1BB antibody in recombinant Chinese hamster ovary cells. Biotechnol Prog. 2003;19:1383-1386.
34. Yamaguchi M, Shirai Y, Inouye Y, Shoji M, Kamei M, Hashizume S, Shirahata S. Changes in monoclonal antibody productivity of recombinant BHK cells immobilized in collagen gel particles. Cytotechnology. 1997;23:5-12.
35. Nam JH, Ermonval M, Sharfstein ST. Cell attachment to microcarriers affects growth, metabolic activity, and culture productivity in bioreactor culture. Biotechnol Prog. 2007;23:652-660.
36. Furukawa K, Ohsuye K. Effect of culture temperature on a recombinant CHO cell line producing a C-terminal a-amidating enzyme. Cytotechnology. 1998;26:153-164.
37. Fox SR, Patel UA, Yap MGS, Wang DIC. Maximizing interferon-production by Chinese hamster ovary cells through temperature shift optimization. Biotechnol Bioeng. 2004;85:177-184
38. Yoon SK, Hwang SO, Lee GM. Enhancing effect of low culture temperature on specific antibody productivity of recombinant Chinese hamster ovary cells: clonal variation. Biotechnol Prog. 2004;20:1683-1688.
39. Clark KJR, Chaplin FWR, Harcum SW. Temperature effects on product-quality-related enzymes in batch CHO cell cultures producing recombinant tPA. Biotechnol Prog. 2004;20:1888-1892.
40. Yoon SK, Hong JK, Choo SH, Song JY, Park HW, Lee GM. Adaptation of Chinese hamster ovary cells to low culture temperature: cell growth and recombinant protein production. J Biotechnol. 2006;122:463-172.
41. Chuppa S, Tsai YS, Yoon S, Shackleford S, Rozales C, Bhat R, Tsay G, Matanguihan C, Konstantinov K, Naveh D. Fermentor temperature as a tool for control of high-density perfusion cultures of mammalian cells. Biotechnol Bioeng. 1997;55:328-338.
42. Ricci MS, Brems DN. Common structural stability properties of 4-helical bundle cytokines: possible physiological and pharmaceutical consequences. Curr Pharm Des. 2004;10:3901-3912.
43. Meng F, Park Y, Zhou, H. Role of proline, glycerol, and heparin as protein folding aids during refolding of rabbit muscle creatine kinase. Int J Biochem Cell Biol. 2001;33:701-709.
44. Kim D, Lee YJ. Effect of glycerol on protein aggregation: quantification of thermal aggregation of proteins from CHO cells and analysis of aggregated proteins. J Therm Biol. 1993;18:41-48.
45. Yoshida H, Yoshizawa T, Shibasaki F, Shoji S, Kanazawa I. Chemical chaperones reduce aggregate formation and cell death caused by the truncated Machado-Joseph disease gene product with an expanded polyglutamine stretch. Neurobiol Dis. 2002;10:88-99.