Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci

Microbial Cell Factories

Volumn 12, Issue 1, 2013, Pages

  Sabri, Suriana ^a   Steen, Jennifer A ^a   Bongers, Mareike ^a   Nielsen, Lars K ^a   Vickers, Claudia E ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Chromosomal integration; csc genes; E. coli; GFP; Homologous recombination; Plasmid; Recombineering; Xylanase

Indexed keywords

BACTERIAL VECTOR; BETA GALACTOSIDASE; CHLORAMPHENICOL; DNA FRAGMENT; GREEN FLUORESCENT PROTEIN; KANAMYCIN; RECOMBINASE; RIBOSE; SUCROSE; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL STRAIN; CHROMOSOME INSERTION; DNA SEQUENCE; ESCHERICHIA COLI; GENE EXPRESSION; GENE LOCUS; METABOLIC ENGINEERING; METABOLISM; MOLECULAR CLONING; NONHUMAN; OPERON; PLASMID;

CHROMOSOMES, BACTERIAL; DNA; ENDO-1,4-BETA XYLANASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE KNOCK-IN TECHNIQUES; GENE KNOCKOUT TECHNIQUES; GENETIC LOCI; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HOMOLOGOUS RECOMBINATION; ION PUMPS; LAC OPERON; MEMBRANE TRANSPORT PROTEINS; METABOLIC NETWORKS AND PATHWAYS; MULTIENZYME COMPLEXES; PLASMIDS; REPRESSOR PROTEINS;

ESCHERICHIA COLI;

EID: 84879225332     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-12-60     Document Type: Article

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