A method for construction of E. coli strains with multiple DNA insertions in the chromosome

Gene

Volumn 187, Issue 2, 1997, Pages 231-238

  Peredelchuk, Michael Y ^a   Bennett, George N ^a  

^a RICE UNIVERSITY   (United States)

Author keywords

Amplification; Excision; Integration; P1 transduction; Phage ; Site specific recombination; Tn1545

Indexed keywords

BACTERIAL DNA; CIRCULAR DNA; DNA FRAGMENT; DNA MARKER; INTEGRASE;

ANTIBIOTIC RESISTANCE; ARTICLE; CHROMOSOME; CLONE; CONTROLLED STUDY; DNA RECOMBINATION; ESCHERICHIA COLI; GENE CONSTRUCT; GENE INSERTION; GENETIC TRANSDUCTION; NONHUMAN; PLASMID; PRIORITY JOURNAL; TRANSPOSON;

CHROMOSOMES, BACTERIAL; DNA TRANSPOSABLE ELEMENTS; DNA, CIRCULAR; ESCHERICHIA COLI; GENETIC MARKERS; LAC OPERON; RECOMBINATION, GENETIC; TRANSFECTION;

BACTERIA (MICROORGANISMS); BACTERIOPHAGE LAMBDA; ESCHERICHIA COLI;

EID: 0030932409     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00760-3     Document Type: Article

References (29)

1. Bolivar, F., Rodriguez, R.L., Greene, P.J., Betlach, M.C., Heynecker, H.L., Boyer, H.W., Crosa, J.H. and Falkaw, S. (1977) Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2, 95-113.
2. Chang, A.C.Y. and Cohen, S.N. (1978) Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the p15A cryptic miniplasmid. J. Bacteriol. 134, 1141-1156.
3. R cassettes with the option of excision of the antibiotic-resistance determinant. Gene 158, 9-14.
4. Chopin, M.-C., Chopin, A., Ronault, A. and Galleron, M. (1989) Insertion and amplification of foreign genes in the Lactococcus lactis subsp. lactis chromosome. Appl. Environ. Microbiol. 55, 1769-1774.
5. Cirillo, J.D., Stover, C.K., Bloom, B.R., Jacobs, W.K. and Barletta, R.J. (1995) Bacterial vaccine vectors and Bacillus Calmette-Guerin. Clin. Infect. Dis. 20, 1001-1009.
6. Clewell, D.B., Flannagan, S.E. and Jaworski, D.D. (1995): Unconstrained bacterial promiscuity: the Tn916-Tn1545 family of conjugative transposons. Trends Microbiol. 3, 229-236.
7. Daniels, D.L., Schroeder, J.L., Szybalski, W., Sanger, F., Coulson, A.R., Hong, G.F., Hill, D.F., Petersen, G.B. and Blattner, F.R. (1983) Appendix II. Complete annotated lambda sequence. In: Hendrix, R.W., Roberts, J.W., Stahl, F.W. and Weisberg, R.A. (Eds.), Lambda II, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp. 519-676.
8. De Lorenzo, V. and Timmis, K.N. (1994) Analysis and construction of stable phenotypes in Gram-negative bacteria with Tn5- and Tn10-derived minitransposons. Methods Enzymol. 235, 386-405.
9. Echols, H. and Guarneros, G. (1983) Control of integration and excision. In: Hendrix, R.W., Roberts, J.W., Stahl, F.W. and Weisberg, R.A. (Eds.), Lambda II, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 75-92.
10. Geissler, S. and Drummond, M. (1993) A counterselectable pACYC184-based lacZα-complementing plasmid vector with novel multiple cloning sites; construction of chromosomal deletions in Klebsiella pneumoniae. Gene 136, 253-255.
11. Kalnins, A., Otto, K., Ruether, U. and Muller-Hill, B. (1983) Sequence of the lacZ gene of Escherichia coli. EMBO J. 2, 593-597.
12. Kiel, J.A.K.W., ten Berge, A.M., Borger, P. and Venema, G. (1995) A general method for the consecutive integration of single copies of a heterologous gene at multiple locations in the Bacillus subtilis chromosome by replacement recombination. Appl. Environ. Microbiol. 61, 4244-4250.
13. Kristensen, C.S., Eberl, L., Sanchez-Romero, J.M., Givskov, M., Molin, S. and De Lorenzo, V. (1995) Site-specific deletions of chromosomally located DNA segments with the multimer resolution system of broad-host-range plasmid RP4. J. Bacteriol. 177, 52-58.
14. Maksimova, T.G., Mustayev, A.A., Zaychikov, E.F., Lyakhov, D.L., Tunitskaya, V.L., Akbarov, A.K., Luchin, S.V., Rechinsky, V.O., Chernov, B.K. and Kochetkov, S.N. (1991) Lys631 residue in the active site of the bacteriophage T7 RNA polymerase. Affinity labeling and site-directed mutagenesis. Eur. J. Biochem. 195, 841-847.
15. Miller, J.H. (1972) Experiments in Molecular Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
16. Mori, M., Hashigushi, K.-I., Yoda, K. and Yamasaki, M. (1988) Designed gene amplification on the Bacillus subtilis chromosome. J. Gen. Microbiol. 134, 85-95.
17. Murray, N.E., Brammar, W.J. and Murray, K. (1977) Lambdoid phages that simplify the recovery of in vitro recombinants. Mol. Gen. Genet. 150, 53-58.
18. Nash, H.A., Enquist, L.W. and Weisberg, R.A. (1977) On the role of the bacteriophage λint gene product in the site specific recombination. J. Mol. Biol. 116, 627-631.
19. Peredelchuk, M.Y., Fedorova, N.D., Smirnov, Y.V. and Debabov, V.G. (1994) Integration of the lysC and asd genes of Corynebacterium glutamicum into the attB site of phage λ on the E. coli chromosome. Biotechnology 1, 12-14.
20. Pósfai, G., Koob, M., Hradečná, Z., Hasan, N., Filutowicz, M. and Szybalski, W. (1994) In vivo excision and amplification of large segments of the genome. Nucleic Acids Res. 22, 2392-2398.
21. Poyart-Salmeron, C., Trieu-Cuot, P., Carlier, C. and Courvalin, P. (1989) Molecular characterization of two proteins involved in the excision of the conjugative transposon Tn1545: homologies with other site-specific recombinases. EMBO J. 8, 2425-2433.
22. Poyart-Salmeron, C., Trieu-Cuot, P., Carlier, C. and Courvalin, P. (1990) The integration-excision system of the conjugative transposon Tn1545 is structurally and functionally related to those of lambdoid phages. Mol. Microbiol. 4, 1513-1521.
23. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
24. Sauer, B. (1994) Recycling selectable marker in yeast. BioTechniques 16, 1086-1088.
25. Simons, R.W., Houman, F. and Kleckner, N. (1987) Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene 53, 85-96.
26. Trieu-Cuot, P., Carlier, C., Poyart-Salmeron, C. and Courvalin, P. (1991) An integrative vector exploiting the transposition properties of Tn1545 for insertional mutagenesis and cloning of genes from Gram-positive bacteria. Gene 106, 21-27.
27. Trieu-Cuot, P., Poyart-Salmeron, C., Carlier, C. and Courvalin, P. (1993) Sequence requirements for target activity in site-specific recombination mediated by the Int protein of transposon Tn1545. Mol. Microbiol. 81, 179-185.
28. Vieira, J. and Messing, J. (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19, 259-268.
29. Yanisch-Perron, C., Vieira, J. and Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119.