MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules

Nature Genetics

Volumn 37, Issue 3, 2005, Pages 311-319

  Li, Mamie Z ^a   Elledge, Stephen J ^a  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; RECOMBINANT DNA; RESTRICTION ENDONUCLEASE;

ARTICLE; BACTERIAL STRAIN; CATALYSIS; CLONING; CONTROLLED STUDY; DNA TRANSFER; ESCHERICHIA COLI; FUNCTIONAL GENOMICS; HOMOLOGOUS RECOMBINATION; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; AMINO ACID SEQUENCE; GENE TRANSFER; GENETICS; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA, RECOMBINANT; GENE TRANSFER TECHNIQUES; MOLECULAR SEQUENCE DATA; PLASMIDS;

EID: 15044348273     PISSN: 10614036     EISSN: 15461718     Source Type: Journal    
DOI: 10.1038/ng1505     Document Type: Article

References (33)

1. Smith, H.O. & Wilcox, K.W. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. J. Mol. Biol. 51, 379-391 (1970).
2. Danna, K. & Nathans, D. Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae. Proc. Natl. Acad. Sci. USA 68, 2913-2917 (1971).
3. Cohen, S.N., Chang, A.C., Boyer, H.W. & Helling, R.B. Construction of biologically functional bacterial plasmids in vitro. Proc. Natl. Acad. Sci. USA 70, 3240-3244 (1973).
4. Backman, K. & Ptashne, M. Maximizing gene expression on a plasmid using recombination in vitro. Cell 13, 65-71 (1978).
5. Uetz, P. et al. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Nature 403, 623-627 (2000).
6. Li, S. et al. A map of the interactome network of the metazoan. C. elegans. Science 303, 540-543 (2004).
7. Ho, Y. et al. Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry. Nature 415, 180-183 (2002).
8. Gavin, A.C. et al. Functional organization of the yeast proteome by systematic analysis of protein complexes. Nature 415, 141-147 (2002).
9. Liu, Q., Li, M.Z., Liebham, D., Cortez, D. & Elledge, S.J. The univector plasmid fusion system, a method for rapid construction of recombinant DNA without restriction enzymes. Curr. Biol. 8, 1300-1309 (1998).
10. Hartley, J.L., Temple, G.F. & Brasch, M.A. DNA cloning using in vitro site-specific recombination. Genome Res. 10, 1788-1795 (2000).
11. Walhout, A.J. et al. GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes. Methods Enzymol. 328, 575-592 (2000).
12. Bethke, B. & Sauer, B. Segmental genomic replacement by Cre-mediated recombination: Genotoxic stress activation of the p53 promoter in single-copy transformants. Nucleic Acids Res. 25, 2828-2834 (1997).
13. Nebert, D.W., Dalton, T.P., Stuart, G.W. & Carvan, M.J. Gene-swap knock-in cassette in mice to study allelic differences in human genes. Ann. NY Acad. Sci. 919, 148-170 (2000).
14. Siegel, R.W. et al. Recombinatorial cloning using heterologous lox sites. Genome Res. 14, 1119-1129 (2004).
15. Frost, L.S., Ippen-Ihler, K. & Skurray, R.A. Analysis of the sequence and gene products of the transfer region of the F sex factor. Microbiol. Rev. 58, 162-210 (1994).
16. Metcalf, W.W., Jiang, W. & Wanner, B.L. Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6K gamma origin plasmids at different copy numbers. Gene 138, 1-7 (1994).
17. Winans, S.C., Elledge, S.J., Mitchell, B.B., Marsh, L. & Walker, G.C. Site directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli. J. Bacteriol. 161, 1219-1221 (1985).
18. Kaiser, K. & Murray, N.E. On the nature of sbcA mutations in E. coli K 12. Mol. Gen. Genet. 179, 555-563 (1980).
19. Yu, D. et al. An efficient recombination system for chromosome engineering in Escherichia coli. Proc. Natl. Acad. Sci. USA 97, 5978-5983 (2000).
20. Datsenko, K.A. & Wanner, B.L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. USA 97, 6640-6645 (2000).
21. Zhang, P., Li, M.Z. & Elledge, S.J. Towards genetic genome projects: genomic library screening and gene targeting vector construction in a single step. Nat. Genet. 30, 31-39 (2002).
22. Hashimoto, T. & Sekiguchi, M. Isolation of temperature-sensitive mutants of R plasmid by in vitro mutagenesis with hydroxylamine. J. Bacteriol. 127, 1561-1563 (1976).
23. Kuzminov, A. Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda. Microbiol. Mol. Biol. Rev. 63, 751-813 (1999).
24. Gumbiner-Russo, L.M., Lombardo, M., Ponder, R.G. & Rosenberg, S.M. The TGV transgenic vectors for single-copy gene expression from the Escherichia coli chromosome. Gene 273, 97-104 (2001).
25. Kast, P. pKSS-a second-generation general purpose cloning vector for efficient positive selection of recombinant clones. Gene 138, 109-114 (1994).
26. Heyneker, H.L. et al. Synthetic lac operator DNA is functional in vivo. Nature 263, 748-752 (1976).
27. Marians, K.J. et al. Cloned synthetic operator DNA is functional in vivo. Nature 263, 744-748 (1976).
28. Haldimann, A., Daniels, L.L. & Wanner, B.L. Use of new methods for construction of tightly regulated arabinose and rhamnose promoter fusions in studies of the Escherichia coli phosphate regulon. J. Bacteriol. 180, 1277-1286 (1998).
29. Shine, J. & Dalgarno, L. Determinant of cistron specificity in bacterial ribosomes. Nature 254, 34-38 (1975).
30. Kozak, M. Circumstances and mechanisms of inhibition of translation by secondary structure in eucaryotic mRNAs. Mol. Cell. Biol. 9, 5134-5142 (1989).
31. Bai, C. et al. SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell 86, 263-274 (1996).
32. Paddison, P.J. et al. A resource for large-scale RNAi based screens in mammals. Nature 428, 427-431 (2004).
33. Cherepanov, P.P. & Wackernagel, W. Gene disruption in Escherichia coli:TcR and KmR cassettes with the option of Flp-mediated excision of the antibiotic determinant. Gene 158, 9-14 (1995).