Point mutation of bacterial artificial chromosomes by ET recombination

EMBO Reports

Volumn 1, Issue 3, 2000, Pages 239-243

  Muyrers, Joep P P ^a   Zhang, Youming ^a   Benes, Vladimir ^a   Testa, Giuseppe ^a   Ansorge, Wilhelm ^a   Stewart, A Francis ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; GLYCOSYLTRANSFERASE; LEVANSUCRASE; RECOMBINANT DNA;

ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; BACTERIAL CHROMOSOME; ESCHERICHIA COLI; GENETIC ENGINEERING; GENETIC RECOMBINATION; GENETICS; METHODOLOGY; PLASMID; POINT MUTATION; SITE DIRECTED MUTAGENESIS;

CHROMOSOMES, ARTIFICIAL, BACTERIAL; CHROMOSOMES, BACTERIAL; DNA, BACTERIAL; DNA, RECOMBINANT; ESCHERICHIA COLI; GENETIC ENGINEERING; HEXOSYLTRANSFERASES; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; POINT MUTATION; RECOMBINATION, GENETIC;

EID: 0034278464     PISSN: 1469221X     EISSN: None     Source Type: Journal    
DOI: 10.1093/embo-reports/kvd049     Document Type: Article

References (16)

1. Angrand, P.O., Daigle, N., van der Hoeven, F., Schoeler, H.R. and Stewart, A.F. (1999) Simplified generation of targeting constructs using ET recombination. Nucleic Acids Res., 27, e16.
2. Baskaran, K., Erfurth, F., Taborn, G., Copeland, N.G., Gilbert, D.J., Jenkins, N.A., lannaccone, P.M. and Domer, P.H. (1997) Cloning and developmental expression of the murine homolog of the acute leukemia proto-oncogene AF4. Oncogene, 15, 1967-1978.
3. Blomfield, I.C., Vaughn, V., Rest, R.F. and Eisenstein, B.I. (1991) Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 replicon. Mol. Microbiol, 5, 1447-1457.
4. Datsenko, K.A. and Wanner, B.L. (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. NatlAcad. Sci. USA, 97, 6640-6645.
5. Guzman, L.M., Belin, D., Carson, M.J. and Beckwith, J. (1995) Tight regulation, modulation, and high-level expression by vectors containing the arabinose pBAD promoter. J. Bacteriol., 177, 4121-4130.
6. Hill, F., Benes, V., Thomasova, D., Stewart, A.F., Kafatos, F.C. and Ansorge, W. (2000) BAC trimming: minimizing clone overlaps. Genomics, 64, 111-113.
7. Ioannou, P.A., Amemiya, C.T., Games, J., Kroisel, P.M., Shizuya, H., Chen, C., Batzer, M.A. and de Jong, P.J. (1994) A new bacteriophage Pl-derived vector for the propagation of large human DNA fragments. Nature Genet., 6, 84-89.
8. Murphy, K.C. (1991) Lambda Gam protein inhibits the helicase and chistimulated recombination activities of Escherichia coli RecBCD enzyme. J. Bacteriol., 173, 5808-5821.
9. Muyrers, J.P.P., Zhang, Y., Testa, G. and Stewart, A.F. (1999) Rapid modification of bacterial artificial chromosomes by ET-recombination. Nucleic Acids Res., 27, 1555-1557.
10. Muyrers, J.P.P., Zhang, Y., Buchholz, F. and Stewart, A.F. (2000a) RecE/RecT and Redo/Redβ initiate double stranded break repair by specifically interacting with their respective partners. Genes Dev., 14, 1971-1982.
11. Muyrers, J.P.P., Zhang, Y. and Stewart, A.F. (2000b) ET-cIoning: Think recombination first. In Setlow, J.K. (ed.), Genetic Engineering, Principles and Methods. Volume 22. Kluwer Academic/Plenum Press, New York, NY, (in press).
12. Narayanan, K., Williamson, R., Zhang, Y., Stewart, A.F. and Ioannou, P.A. (1999) Efficient and precise engineering of a 200 kb β-globin human/bacterial artificial chromosome in E.coli DH10B using an inducible homologous recombination system. Gene Ther., 6, 442-447.
13. Shizuya, H., Birren, B., Kim, U.J., Mancino, V., Slepak, T., Tachiiri, Y. and Simon, M. (1992) Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc. Natl Acad. Sci. USA, 89, 8794-8797.
14. Sternberg, N.L. (1992) Cloning high molecular weight DNA fragments by the bacteriophage PI system. Trends Genet., 8, 11-16.
15. Yu, D., Ellis, H.M., Lee, E.C., Jenkins, N.A., Copeland, N.G. and Court, D.L. (2000) An efficient recombination system for chromosome engineering in Escherichia coli. Proc. Natl Acad. Sci. USA, 97, 5978-5983.
16. Zhang, Y., Buchholz, F., Muyrers, J.P.P. and Stewart, A.F. (1998) A new logic for DNA engineering using recombination in Escherichia coli. Nature Genet., 20, 123-128.