Lambda red-mediated mutagenesis and efficient large scale affinity purification of the Escherichia coli NADH:Ubiquinone oxidoreductase (complex I)

Biochemistry

Volumn 46, Issue 37, 2007, Pages 10694-10702

  Pohl, Thomas ^a   Uhlmann, Mareike ^a,b   Kaufenstein, Miriam ^a   Friedrich, Thorsten ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

^b Invitrogen Ltd   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DEHYDROGENASES; NUO-OPERON; PROTON-PUMPING;

ELECTRON TRANSITIONS; ENZYME ACTIVITY; GENES; MUTAGENESIS; PROTONS; PURIFICATION; SOLUBILITY;

BACTERIA;

ARABINOSE; DIGLYCINE; DODECYL MALTOSIDE; HEXAHISTIDINE; LAMBDA RED; LIPID; MALTOSE; NICKEL; PROTEIN SUBUNIT; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE (UBIQUINONE); UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GENE; CATALYSIS; CYTOPLASM; ELECTRON TRANSPORT; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SUBUNIT; ESCHERICHIA COLI; LIPID COMPOSITION; MOLECULAR CLONING; NONHUMAN; NUO GENE; OPERON; PRIORITY JOURNAL; PROMOTER REGION; PROTEOLIPOSOME; SOLUBILIZATION;

CHROMATOGRAPHY, AFFINITY; ELECTRON SPIN RESONANCE SPECTROSCOPY; ELECTRON TRANSPORT COMPLEX I; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENETIC ENGINEERING; HYDROGEN-ION CONCENTRATION; MUTAGENESIS;

ESCHERICHIA COLI;

EID: 34548683897     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi701057t     Document Type: Article

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