A PCR-free cloning method for the targeted φ80 Int-mediated integration of any long DNA fragment, bracketed with meganuclease recognition sites, into the Escherichia coli chromosome

Journal of Microbiological Methods

Volumn 89, Issue 3, 2012, Pages 167-173

  Ublinskaya, Anna A ^a   Samsonov, Valeriy V ^a   Mashko, Sergey V ^a   Stoynova, Nataliya V ^a  

^a Ajinomoto Genetika Research Institute   (Russian Federation)

Author keywords

DNA cloning; Escherichia coli; I SceI endonuclease; Integration; Recombineering

Indexed keywords

DNA FRAGMENT; ENDONUCLEASE; I SCEI ENDONUCLEASE; PLASMID VECTOR; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIOPHAGE; CONTROLLED STUDY; COPY NUMBER VARIATION; DNA BRACKETED REGION; DNA FLANKING REGION; DNA INTEGRATION; DNA RECOMBINATION; DNA STRUCTURE; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENE INSERTION; GENE LOCUS; GENE STRUCTURE; GENE TARGETING; MOLECULAR CLONING; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION SITE;

BACTERIOPHAGES; CLONING, MOLECULAR; DNA, BACTERIAL; ESCHERICHIA COLI; GENE TARGETING; GENETIC ENGINEERING; INTEGRASES; MUTAGENESIS, INSERTIONAL; RECOMBINATION, GENETIC;

BACTERIOPHAGE PHI-80; ESCHERICHIA COLI;

EID: 84860376073     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2012.03.013     Document Type: Article

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