Large-scale production and study of a synthetic G protein-coupled receptor: Human olfactory receptor 17-4

Proceedings of the National Academy of Sciences of the United States of America

Volumn 106, Issue 29, 2009, Pages 11925-11930

  Cook, Brian L ^a   Steuerwald, Dirk ^a   Kaiser, Liselotte ^a   Graveland Bikker, Johanna ^a   Vanberghem, Melanie ^a   Berke, Allison P ^a   Herlihy, Kara ^b   Pick, Horst ^c   Vogel, Horst ^c   Zhang, Shuguang ^a  

^a Massachusetts Institute of Technology Cambridge   (United States)

^b GE HEALTHCARE   (United States)

^c EPFL   (Switzerland)

Author keywords

BiacoreA100; Detergent screen; Fos choline 14; G protein coupled receptor purification; Membrane protein

Indexed keywords

BETA (4 TERT BUTYLPHENYL) ALPHA METHYLPROPIONALDEHYDE; CALCIUM ION; CHEMICAL AGENT; DETERGENT; FLORALOZONE; G PROTEIN COUPLED RECEPTOR; HUMAN OLFACTORY RECEPTOR 17-4; MONOMER; RECEPTOR PROTEIN; TETRACYCLINE; UNCLASSIFIED DRUG;

ALPHA HELIX; ANTIBODY AFFINITY; ARTICLE; BIOREACTOR; CALCIUM MOBILIZATION; CIRCULAR DICHROISM; CONTROLLED STUDY; FEASIBILITY STUDY; GEL PERMEATION CHROMATOGRAPHY; HUMAN; HUMAN CELL; IN VITRO STUDY; LARGE SCALE PRODUCTION; MASS SPECTROMETRY; MOLECULAR INTERACTION; MOLECULAR RECOGNITION; OLFACTORY NERVE; OLFACTORY SYSTEM; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SCALE UP; SIGNAL TRANSDUCTION; SMELLING; SURFACE PLASMON RESONANCE; SUSPENSION CELL CULTURE;

BIOLOGICAL ASSAY; BIOREACTORS; CALCIUM; CELL LINE; DETERGENTS; HUMANS; INTRACELLULAR SPACE; PROTEIN BINDING; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECEPTORS, G-PROTEIN-COUPLED; RECEPTORS, ODORANT; SEMINAL PLASMA PROTEINS; SOLUBILITY; SPECTRUM ANALYSIS; SURFACE PLASMON RESONANCE;

MAMMALIA;

EID: 67749133998     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0811089106     Document Type: Article

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