Design of an expression system for detecting folded protein domains and mapping macromolecular interactions by NMR

Protein Science

Volumn 6, Issue 11, 1997, Pages 2359-2364

  Huth, Jeffrey R ^a   Bewley, Carole A ^a   Jackson, Belinda M ^b   Hinnebusch, Alan G ^b   Clore, G Marius ^a   Gronenborn, Angela M ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

Author keywords

Domain structure; GBI fusion protein; Intermolecular interactions; NMR

Indexed keywords

BACTERIAL PROTEIN; DNA; HYBRID PROTEIN;

ARTICLE; EXPRESSION VECTOR; MOLECULAR INTERACTION; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN STABILITY; PROTEIN STRUCTURE;

BACTERIA (MICROORGANISMS);

EID: 0030691041     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560061109     Document Type: Article

References (24)

1. 15N nuclear magnetic resonance spectroscopy. Protein Sci 3:15-21.
2. Bax A, Ikura M, Kay LE, Torchia DA, Tschudin R. 1990. Comparison of different modes of two-dimensional reverse correlation NMR for the study of proteins. J Magn Reson 86:304-318.
3. Clubb RT, Omichinski JG, Clore GM, Gronenborn AM. 1994. Mapping the binding surface of interleukin-8 complexes with an N-terminal fragment of the type 1 human interleukin-8 receptor. FEBS Lett 338:93-97.
4. Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A. 1995. NMRPipe: A multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6:277-293.
5. di Guan C, Li P, Riggs PD, Inouye H. 1988. Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein. Gene 67:21-30.
6. Garrett DS, Powers R, Gronenborn AM, Clore GM. 1991. A common sense approach to peak picking in two-, three-and four-dimensional spectra using automatic computer analysis of contour diagrams. J Magn Reson 95:214-220.
7. Gronenborn AM, Clore GM. 1996. Rapid screening for structural integrity of expressed proteins by heteronuclear NMR spectroscopy. Protein Sci 5:174-177.
8. Gronenborn AM, Filpula DR, Essig NZ, Achari A, Whitlow M, Wingfield PT, Clore GM. 1991. A novel, highly stable fold of the immunoglobulin binding domain of streptococcal protein G. Science 253:657-661.
9. Jackson BM, Drysdale CM, Natarajan K, Hinnebusch AG. 1996. Identification of seven hydrophobic clusters in GCN4 making redundant contributions to transcriptional activation. Mol Cell Biol 16:5557-5571.
10. 13C-enriched fusion proteins in Escherichia coli. J Biomol NMR 7:131-141.
11. Huang B, Eberstadt M, Olejniczak ET, Meadows RP, Fesik SW. 1996. NMR structure and mutagenesis of the Fas (APO-1/CD95) death domain. Nature 384:638-641.
12. Huth JR, Bewley CA, Nissen MS, Evans JNS, Reeves R, Gronenborn AM, Clore GM. 1997. The solution structure of an HMG-I(Y)/DNA complex defines a new architectural minor groove binding motif. Nature Struct Biol 4:657-665.
13. Huth JR, Norton SE, Lockridge O, Shikone T, Hsueh AJW, Ruddon RW. 1994. Bacterial expression and in vitro folding of the β-subunit of human chorionic gonadotropin (hCGβ) and functional assembly of recombinant hCGβ with hCGα. Endocrinology 135:911-918.
14. LaVallic ER, DiBlasio EA, Kovacic S, Grant KL, Schendel PF, McCoy JM. 1993. A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm. Bio/Technology 11:187-193.
15. Nilsson B, Abrahmsén L, Uhlén M. 1985. Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors EMBO J 4:1075-1080.
16. Omichinski JG, Pedone PV, Felsenfeld G, Gronenborn AM, Clore GM. 1997. The solution structure of a specific GAGA factor-DNA complex reveals a modular binding mode. Nature Struct Biol 4:122-132.
17. Piotto M, Saudek V, Sklenar V. 1992. Gradient-tailored excitation for single quantum NMR spectroscopy of aqueous solutions. J Biomol NMR 2:661-665.
18. Smith DB, Johnson KS. 1988. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67:31-40.
19. Smith CK, Withka JM, Regan L. 1994. A thermodynamic scale for the β-sheet forming tendencies of the amino acids. Biochemistry 33:5510-5517.
20. Studier FW, Moffatt BA. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189:113-130.
21. Suda T, Takahashi T, Golstein P, Nagata S. 1993. Molecular cloning and expression of the Fas ligand, a novel member of the tumor necrosis factor family. Cell 75:1169-1178.
22. Thanos D, Maniatis T. 1992. The high mobility group protein HMG I(Y) is required for NF-β-dependent virus induction of the human IFN-β gene. Cell 71:777-789.
23. Werner MH, Clore GM, Gronenborn AM, Kondoh A, Fisher RJ. 1994. Refolding proteins by gel filtration chromatography. FEBS Lett. 345:125-130.
24. Werner MH, Huth JR, Gronenborn AM, Clore GM. 1995. Molecular basis of human 46X,Y sex reversal revealed from the three-dimensional solution structure of the human SRY-DNA complex. Cell 81:705-114.