Enhancement of recombinant soluble dengue virus 2 envelope domain III protein production in Escherichia coli trxB and gor double mutant

Journal of Bioscience and Bioengineering

Volumn 102, Issue 4, 2006, Pages 333-339

  Saejung, Wanida ^a   Puttikhunt, Chunya ^b   Prommool, Tanapan ^b   Sojikul, Punchapat ^c   Tanaka, Ryoji ^a   Fujiyama, Kazuhito ^a   Malasit, Prida ^b,d   Seki, Tatsuji ^a  

^a OSAKA UNIVERSITY   (Japan)

^b NATIONAL SCIENCE AND TECHNOLOGY DEVELOPMENT AGENCY   (Thailand)

^c MAHIDOL UNIVERSITY   (Thailand)

^d THAMMASAT UNIVERSITY   (Thailand)

Author keywords

antigenicity; dengue virus type 2; envelope domain III protein; Escherichia coli; Origami

Indexed keywords

ANTIBODIES; DISEASE CONTROL; ESCHERICHIA COLI; THERMAL EFFECTS; VIRUSES;

ANTIGENICITY; DENGUE VIRUS TYPE 2; ENVELOPE DOMAIN III PROTEIN; ORIGAMI;

PROTEINS;

AMINO ACID; DENGUE VACCINE; DENGUE VIRUS 2 ENVELOPE DOMAIN III PROTEIN; GLUTATHIONE REDUCTASE; PROTEIN PRECURSOR; RECOMBINANT ANTIGEN; THIOREDOXIN REDUCTASE; UNCLASSIFIED DRUG; VIRUS ANTIBODY; VIRUS ENVELOPE PROTEIN;

AMINO ACID COMPOSITION; ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ANTIBODY RESPONSE; ANTIGEN RECOGNITION; ARTICLE; BACTERIAL GENOME; BACTERIAL STRAIN; BACTERIUM MUTANT; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DENGUE; DENGUE VIRUS; ESCHERICHIA COLI; GENE MUTATION; HUMAN; IMMUNIZATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN SECRETION; PROTEIN SYNTHESIS; RAT; SOLUBILITY; TEMPERATURE DEPENDENCE; VACCINE PRODUCTION;

ANIMALS; DENGUE VIRUS; ESCHERICHIA COLI; GENETIC ENHANCEMENT; PROTEIN ENGINEERING; RATS; RECOMBINANT PROTEINS; SOLUBILITY; VIRAL ENVELOPE PROTEINS;

DENGUE VIRUS; DENGUE VIRUS TYPE 2; ESCHERICHIA COLI; FLAVIVIRUS;

EID: 33751257489     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.102.333     Document Type: Article

References (24)

1. Monath T.P. Dengue: the risk to developed and developing countries. Proc. Natl. Acad. Sci. USA 91 (1994) 2395-2400
2. Chang G.-J. Molecular biology of dengue viruses. In: Gubler D.J., and Kuno G. (Eds). Dengue and dengue hemorrhagic fever (1997), The University Press, Cambridge 175-198
3. Modis Y., Ogata S., Clements D., and Harrison S.C. A ligand-binding pocket in the dengue virus envelope glycoprotein. Proc. Natl. Acad. Sci. USA 100 (2003) 6986-6991
4. Hung J.-J., Hsieh M.-T., Young M.-J., Kao C.-L., King C.-C., and Chang W. An external loop region of domain III of dengue virus type 2 envelope protein is involved in serotype-specific binding to mosquito but not mammalian cells. J. Virol. 78 (2004) 378-388
5. Crill W.D., and Roehrig J.T. Monoclonal antibodies that bind to domain III of dengue virus E glycoprotein are the most efficient blockers of virus adsorption to vero cells. J. Virol. 75 (2001) 7769-7773
6. Simmons M., Nelson W.M., Wu S.J.L., and Hayes C.G. Evaluation of the protective efficacy of a recombinant dengue envelope B domain fusion protein against dengue 2 virus infection in mice. Am. J. Trop. Med. Hyg. 58 (1998) 655-662
7. Sugrue R.J., Cui T., Xu Q., Fu J., and Chan Y.C. The production of recombinant dengue virus E protein using Escherichia coli and Pichia pastoris. J. Virol. Methods 69 (1997) 159-169
8. Jaiswal S., Khanna N., and Swaminathan S. High-level expression and one-step purification of recombinant dengue virus type 2 envelope domain III protein in Escherichia coli. Protein Expr. Purif. 33 (2004) 80-91
9. Bisht H., Chugh D.A., Swaminathan S., and Khanna N. Expression and purification of dengue virus type 2 envelope protein as a fusion with hepatitis B surface antigen in Pichia pastoris. Protein Expr. Purif. 23 (2001) 84-96
10. Wei H.-Y., Jiang L.-F., Xue Y.-H., Fang D.-Y., and Guo H.-Y. Secreted expression of dengue virus type 2 full-length envelope glycoprotein in Pichia pastoris. J. Virol. Methods 109 (2003) 17-23
11. Kelly E.P., Greene J.J., King A.D., and Innis B.L. Purified dengue 2 virus envelope glycoprotein aggregates produced by baculovirus are immunogenic in mice. Vaccine 18 (2000) 2549-2559
12. Men R., Wyatt L., Tokimatsu I., Arakaki S., Shameem G., Elkins R., Chanock R., Moss B., and Lai C.-J. Immunization of rhesus monkeys with a recombinant of modified vaccinia virus Ankara expressing a truncated envelope glycoprotein of dengue type 2 virus induced resistance to dengue type 2 virus challenge. Vaccine 18 (2000) 3113-3122
13. Fonseca B.A., Khoshnood K., Shope R.E., and Mason P.W. Flavivirus type-specific antigens produced from fusions of a portion of the E protein gene with the Escherichia coli trpE gene. Am. J. Trop. Med. Hyg. 44 (1991) 500-508
14. Trirawatanapong T., Chandran B., Putnak R., and Padmanabhan R. Mapping of a region of dengue virus type-2 glycoprotein required for binding by a neutralizing monoclonal antibody. Gene 116 (1992) 139-150
15. Han K.G., Lee S.S., and Kang C. Soluble expression of cloned phage K11 RNA polymerase gene in Escherichia coli at a low temperature. Protein Expr. Purif. 16 (1999) 103-108
16. Shirano Y., and Shibata D. Low temperature cultivation of Escherichia coli carrying a rice lipoxygenase L-2 cDNA produces a soluble and active enzyme at a high level. FEBS Lett. 271 (1990) 128-130
17. Sorensen H.P., and Mortensen K.K. Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli. Microb. Cell Fact. 4 (2005) 1-8
18. Prinz W.A., Aslund F., Holmgren A., and Beckwith J. The role of the thioredoxin and glutaredoxin pathways in reducing protein disulfide bonds in the Escherichia coli cytoplasm. J. Biol. Chem. 272 (1997) 15661-15667
19. Roehrig J.T., Volpe K.E., Squires J., Hunt A.R., Davis B.S., and Chang G.-J.J. Contribution of disulfide bridging to epitope expression of the dengue type 2 virus envelope glycoprotein. J. Virol. 78 (2004) 2648-2652
20. Medina-Godoy S., Nielsen N.C., and Paredes-Lopez O. Expression and characterization of a His-tagged 11S seed globulin from Amaranthus hypochondriacus in Escherichia coli. Biotechnol. Prog. 20 (2004) 1749-1756
21. Dutta S., Ware L.A., Barbosa A., Ockenhouse C.F., and Lanar D.E. Purification, characterization, and immunogenicity of a disulfide cross-linked Plasmodium vivax vaccine candidate antigen, merozoite surface protein 1, expressed in Escherichia coli. Infect. Immun. 69 (2001) 5464-5470
22. Roehrig J.T., Bolin R.A., and Kelly R.G. Monoclonal antibody mapping of the envelope glycoprotein of the dengue 2 virus. Virology 246 (1998) 317-328
23. Gubler D.J. Dengue and dengue hemorrhagic fever. Clin. Microbiol. Rev. 11 (1998) 480-496
24. Chiu M.-W., and Yang Y.-L. Blocking the dengue virus 2 infections on BHK-21 cells with purified recombinant dengue virus 2 E protein expressed in Escherichia coli. Biochem. Biophys. Res. Commun. 309 (2003) 672-678