Dual expression system suitable for high-throughput fluorescence-based screening and production of soluble proteins

Journal of Proteome Research

Volumn 4, Issue 6, 2005, Pages 2137-2147

  Braud, Sandrine ^a   Moutiez, Mireille ^a   Belin, Pascal ^a   Abello, Nicolas ^a,b   Drevet, Pascal ^a   Zinn Justin, Sophie ^a   Courçon, Marie ^a   Masson, Cédric ^a   Dassa, Janie ^a   Charbonnier, Jean Baptiste ^a   Boulain, Jean Claude ^a   Ménez, André ^a   Genet, Roger ^a   Gondry, Muriel ^a  

^a CEA SACLAY   (France)

^b UNIVERSITY MEDICAL CENTER GRONINGEN   (Netherlands)

Author keywords

Amber suppressor; Fluorescence; Fusion proteins; High throughput screening; Protein expression; Recombination cloning; S tag

Indexed keywords

BACTERIAL PROTEIN; DNA FRAGMENT; HYBRID PROTEIN; TRANSFER RNA;

ARTICLE; BACTERIUM CULTURE; ESCHERICHIA COLI; FLUORESCENCE; HIGH THROUGHPUT SCREENING; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEOMICS; SOLUBILITY;

BLOTTING, WESTERN; CLONING, MOLECULAR; CODON, TERMINATOR; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION; GENE EXPRESSION REGULATION, BACTERIAL; GENES, SUPPRESSOR; GENETIC VECTORS; GLUTATHIONE TRANSFERASE; PROTEINS; PROTEOME; PROTEOMICS; RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC; RNA, TRANSFER; SOLUBILITY; SPECTROMETRY, FLUORESCENCE; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; TOBACCO ETCH VIRUS;

EID: 29144453446     PISSN: 15353893     EISSN: None     Source Type: Journal    
DOI: 10.1021/pr050230i     Document Type: Article

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