High yield expression and purification of isotopically labelled human endothelin-1 for use in NMR studies

Protein Expression and Purification

Volumn 48, Issue 2, 2006, Pages 253-260

  Mac, Thien Thi ^a   Beyermann, Michael ^a   Pires, José Ricardo ^b   Schmieder, Peter ^a   Oschkinat, Hartmut ^a  

^a LEIBNIZ INSTITUT FÜR MOLEKULARE PHARMAKOLOGIE   (Germany)

^b FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

Author keywords

Disulfides; Endothelin 1; Isotopic labelling; Peptide production

Indexed keywords

ENDOTHELIN 1; HYBRID PROTEIN; NITROGEN;

ARTICLE; BIOSYNTHESIS; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; ISOTOPE LABELING; MASS SPECTROMETRY; NUCLEAR MAGNETIC RESONANCE; POLYACRYLAMIDE GEL ELECTROPHORESIS;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOTHELIN-1; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; ISOTOPE LABELING; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

ESCHERICHIA COLI;

EID: 33746377576     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.01.022     Document Type: Article

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