Combinatorial strategy of sorbitol feeding and low-temperature induction leads to high-level production of alkaline β-mannanase in Pichia pastoris

Enzyme and Microbial Technology

Volumn 49, Issue 4, 2011, Pages 407-412

  Zhu, Taicheng ^a   You, Lijin ^a   Gong, Fuyu ^a   Xie, Minfeng ^a   Xue, Yanfen ^a   Li, Yin ^a   Ma, Yanhe ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

Author keywords

Alkaline mannanase; Co feeding; High level production; Multi copy; Pichia pastoris

Indexed keywords

CO-FEEDING; HIGH-LEVEL PRODUCTION; MANNANASE; MULTI-COPY; PICHIA PASTORIS;

ALCOHOLS; BACTERIOLOGY; ENZYMES; FEEDING; GENES; YEAST;

LOW TEMPERATURE PRODUCTION;

BETA MANNOSIDASE; SORBITOL;

ARTICLE; CELL DENSITY; ENZYME SYNTHESIS; FEEDING; FERMENTATION; GENE EXPRESSION; LOW TEMPERATURE; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS;

BACILLUS; BETA-MANNOSIDASE; BIOREACTORS; BIOTECHNOLOGY; COLD TEMPERATURE; FERMENTATION; HYDROGEN-ION CONCENTRATION; PICHIA; RECOMBINANT PROTEINS; SORBITOL;

BACILLUS SP.; PICHIA PASTORIS;

EID: 80051790743     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2011.06.022     Document Type: Article

References (26)

1. Dhawan S., Kaur J. Microbial mannanases: an overview of production and applications. Crit Rev Biotechnol 2007, 27:197-216.
2. Song J.M., Nam K.W., Kang S.G., Kim C.G., Kwon S.T., Lee Y.H. Molecular cloning and characterization of a novel cold-active β-1, 4-d-mannanase from the Antarctic springtail, Cryptopygus antarcticus. Comp Biochem Physiol B Biochem Mol Biol 2008, 151:32-40.
3. Perret S., Belaich A., Fierobe H.P., Belaich J.P., Tardif C. Towards designer cellulosomes in clostridia: mannanase enrichment of the cellulosomes produced by Clostridium cellulolyticum. J Bacteriol 2004, 186:6544-6552.
4. Hatada Y., Takeda N., Hirasawa K., Ohta Y., Usami R., Yoshida Y., et al. Sequence of the gene for a high-alkaline mannanase from an alkaliphilic Bacillus sp strain JAMB-750, its expression in Bacillus subtilis and characterization of the recombinant enzyme. Extremophiles 2005, 9:497-500.
5. Yoon K.H., Lim B.L. Cloning and strong expression of a Bacillus subtilis WL-3 mannanase gene in B. subtilis. J Microbiol Biotechnol 2007, 17:1688-1694.
6. Setati M.E., Ademark P., van Zyl W.H., Hahn-Hagerdal B., Stalbrand H. Expression of the Aspergillus aculeatus endo-β-1,4-mannanase encoding gene (man1) in Saccharomyces cerevisiae and characterization of the recombinant enzyme. Protein Expr Purif 2001, 21:105-114.
7. Gellissen G. Heterologous protein production in methylotrophic yeasts. Appl Microbiol Biotechnol 2000, 54:741-750.
8. Daly R., Hearn M.T. Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production. J Mol Recognit 2005, 18:119-138.
9. Xu B., Sellos D., Janson J.C. Cloning and expression in Pichia pastoris of a blue mussel (Mytilus edulis) β-mannanase gene. Eur J Biochem 2002, 269:1753-1760.
10. Luo H., Wang Y., Wang H., Yang J., Yang Y., Huang H., et al. A novel highly acidic β-mannanase from the acidophilic fungus Bispora sp. MEY-1: gene cloning and overexpression in Pichia pastoris. Appl Microbiol Biotechnol 2009, 82:453-461.
11. Chen X., Cao Y., Ding Y., Lu W., Li D. Cloning, functional expression and characterization of Aspergillus sulphureus β-mannanase in Pichia pastoris. J Biotechnol 2007, 128:452-461.
12. Ma Y., Xue Y., Dou Y., Xu Z., Tao W., Zhou P. Characterization and gene cloning of a novel β-mannanase from alkaliphilic Bacillus sp. N16-5. Extremophiles 2004, 8:447-454.
13. He X., Liu N., Li W., Zhang Z., Zhang B., Ma Y. Inducible and constitutive expression of a novel thermostable alkaline β-mannanase from alkaliphilic Bacillus sp. N16-5 in Pichia pastoris and characterization of the recombinant enzyme. Enzyme Microb Technol 2008, 43:13-18.
14. Vassileva A., Chugh D.A., Swaminathan S., Khanna N. Effect of copy number on the expression levels of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris. Protein Expr Purif 2001, 21:71-80.
15. Mansur M., Cabello C., Hernandez L., Pais J., Varas L., Valdes J., et al. Multiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastoris. Biotechnol Lett 2005, 27:339-345.
16. Zhu T., Guo M., Tang Z., Zhang M., Zhuang Y., Chu J., et al. Efficient generation of multi-copy strains for optimizing secretory expression of porcine insulin precursor in yeast Pichia pastoris. J Appl Microbiol 2009, 107:954-963.
17. D'Anjou M.C., Daugulis A.J. A rational approach to improving productivity in recombinant Pichia pastoris fermentation. Biotechnol Bioeng 2001, 72:1-11.
18. Jungo C., Marison I., von Stockar U. Regulation of alcohol oxidase of a recombinant Pichia pastoris Mut+ strain in transient continuous cultures. J Biotechnol 2007, 130:236-246.
19. Inan M., Aryasomayajula D., Sinha J., Meagher M.M. Enhancement of protein secretion in Pichia pastoris by overexpression of protein disulfide isomerase. Biotechnol Bioeng 2006, 93:771-778.
20. Ma Y., Hendershot L.M. The unfolding tale of the unfolded protein response. Cell 2001, 107:827-830.
21. Travers K.J., Patil C.K., Wodicka L., Lockhart D.J., Weissman J.S., Walter P. Functional and genomic analyses reveal an essential coordination between the unfolded protein response and ER-associated degradation. Cell 2000, 101:249-258.
22. Ramon R., Ferrer P., Valero F. Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris. J Biotechnol 2007, 130:39-46.
23. Celik E., Calik P., Oliver S.G. Fed-batch methanol feeding strategy for recombinant protein production by Pichia pastoris in the presence of co-substrate sorbitol. Yeast 2009, 26:473-484.
24. Inan M., Meagher M.M. Non-repressing carbon sources for alcohol oxidase (AOX1) promoter of Pichia pastoris. J Biosci Bioeng 2001, 92:585-589.
25. Chung B.K., Selvarasu S., Andrea C., Ryu J., Lee H., Ahn J., et al. Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement. Microb Cell Fact 2010, 9:50.
26. Gasser B., Saloheimo M., Rinas U., Dragosits M., Rodriguez-Carmona E., Baumann K., et al. Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview. Microb Cell Fact 2008, 7:11.