Analysis of nonsense-mediated mRNA decay in mammalian cells

Current Protocols in Cell Biology

Volumn , Issue SUPPL.55, 2012, Pages

  Nicholson, Pamela ^a   Joncourt, Raphael ^a   Mühlemann, Oliver ^a  

^a UNIVERSITY OF BERN   (Switzerland)

Author keywords

Complementation assay; NMD; RNA; RNAi; RT qPCR; Site directed mutagenesis; Tethered function assay

Indexed keywords

AMINO ACID; AMPICILLIN; ANTIBIOTIC AGENT; CYSTEINE; DNA BINDING PROTEIN; DNA DIRECTED RNA POLYMERASE III; DNA POLYMERASE; DOUBLE STRANDED DNA; ENDONUCLEASE; ENDONUCLEASE DPN1; ETHIDIUM BROMIDE; FLUORESCENT DYE; GLYCINE; IMMUNOGLOBULIN; KANAMYCIN; LOCKED NUCLEIC ACID; MESSENGER RNA; MICRORNA; MUTANT PROTEIN; OLIGONUCLEOTIDE; PLASMID DNA; POLYPROPYLENE; RIBONUCLEASE; RIBONUCLEASE A; RNA; SHORT HAIRPIN RNA; SMALL INTERFERING RNA; SYBR GREEN DYE; T LYMPHOCYTE RECEPTOR; UNCLASSIFIED DRUG; UNINDEXED DRUG; STOP CODON;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; AGAR MEDIUM; AMINO ACID SEQUENCE; AMPLICON; ANIMAL CELL; ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; BINDING SITE; CELL FUNCTION; CELL TRANSFORMATION; CELLULAR DISTRIBUTION; CHEMICAL ANALYSIS; CODON; DIMERIZATION; DNA BINDING; DNA DENATURATION; DNA METHYLATION; DNA SEQUENCE; DNA TEMPLATE; ENZYME ACTIVITY; ENZYME ASSAY; ESCHERICHIA COLI; FAST POLYNUCLEOTIDE LIQUID CHROMATOGRAPHY; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE EXPRESSION; GENE FREQUENCY; GENE SILENCING; GENE TARGETING; GENE VECTOR; LABELING INDEX; LIQUID CHROMATOGRAPHY; LYMPHOCYTE; MAMMAL CELL; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR BIOLOGY; NONHUMAN; NONSENSE MEDIATED MRNA DECAY; OPEN READING FRAME; PHOTOCHEMICAL QUENCHING; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; POLYMERIZATION; PREMATURE TRANSLATION TERMINATION CODON; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN HYDROLYSIS; PROTEIN PROTEIN INTERACTION; PROTEIN SYNTHESIS; QUANTITATIVE ANALYSIS; REPORTER GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA DEGRADATION; RNA EXTRACTION; RNA INTERFERENCE; RNA TRANSLATION; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS; STRATEGIC PLANNING; TISSUE CULTURE; WILD TYPE; ANIMAL; CAENORHABDITIS ELEGANS; GENETICS; HUMAN; METABOLISM; MOUSE; PROCEDURES; STOP CODON; TISSUE CULTURE TECHNIQUE;

EUKARYOTA; MAMMALIA;

ANIMALS; CAENORHABDITIS ELEGANS; CODON, NONSENSE; GENES, REPORTER; HUMANS; MICE; MUTAGENESIS, SITE-DIRECTED; NONSENSE MEDIATED MRNA DECAY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA INTERFERENCE; RNA, MESSENGER; TISSUE CULTURE TECHNIQUES;

EID: 84868260851     PISSN: 19342500     EISSN: 19342616     Source Type: Journal    
DOI: 10.1002/0471143030.cb2704s55     Document Type: Article

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