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Volumn 1, Issue 2, 2006, Pages 581-585

The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: Twenty-something years on

Author keywords

[No Author keywords available]

Indexed keywords

CHLOROFORM; GUANIDINE DERIVATIVE; GUANIDINE THIOCYANATE; PHENOL; RNA; THIOCYANIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

EID: 34248663955     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2006.83     Document Type: Article
Times cited : (1609)

References (7)
  • 1
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    • Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
    • Chirgwin, J.M., Przybyla, A.E., MacDonald, R.J. & Rutter, W.J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18, 5294-5299 (1979).
    • (1979) Biochemistry , vol.18 , pp. 5294-5299
    • Chirgwin, J.M.1    Przybyla, A.E.2    MacDonald, R.J.3    Rutter, W.J.4
  • 2
    • 77956995425 scopus 로고
    • The use of guanidine chloride in the isolation of nucleic acids
    • Cox, R.A. The use of guanidine chloride in the isolation of nucleic acids. Methods Enzymol. 12, 120-129 (1968).
    • (1968) Methods Enzymol , vol.12 , pp. 120-129
    • Cox, R.A.1
  • 3
    • 0023277545 scopus 로고
    • Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
    • Chomczynski, P. & Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159 (1987).
    • (1987) Anal. Biochem , vol.162 , pp. 156-159
    • Chomczynski, P.1    Sacchi, N.2
  • 4
    • 0025137467 scopus 로고
    • An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
    • Puissant, C. & Houdebine, L.M. An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Biotechniques 8, 148-149 (1990).
    • (1990) Biotechniques , vol.8 , pp. 148-149
    • Puissant, C.1    Houdebine, L.M.2
  • 5
    • 0026654006 scopus 로고
    • Solubilization in formamide protects RNA from degradation
    • Chomczynski, P. Solubilization in formamide protects RNA from degradation. Nucleic Acids Res. 20, 3791-3792 (1992).
    • (1992) Nucleic Acids Res , vol.20 , pp. 3791-3792
    • Chomczynski, P.1
  • 7
    • 0030895171 scopus 로고    scopus 로고
    • Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity
    • Wilfinger, W. W., Mackey, K. & Chomczynski, P. Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity. Biotechniques 22, 474-6, 478-81 (1997).
    • (1997) Biotechniques , vol.22
    • Wilfinger, W.W.1    Mackey, K.2    Chomczynski, P.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.