-
1
-
-
27144468030
-
Reliable gene expression measurements from degraded RNA by quantitative realtime PCR depend on short amplicons and a proper normalization
-
Antonov J, Goldstein DR, Oberli A, Baltzer A, Pirotta M, Fleischmann A, Altermatt HJ, Jaggi R. 2005. Reliable gene expression measurements from degraded RNA by quantitative realtime PCR depend on short amplicons and a proper normalization. Laboratory Investigation 85, 1040-1050.
-
(2005)
Laboratory Investigation
, vol.85
, pp. 1040-1050
-
-
Antonov, J.1
Goldstein, D.R.2
Oberli, A.3
Baltzer, A.4
Pirotta, M.5
Fleischmann, A.6
Altermatt, H.J.7
Jaggi, R.8
-
2
-
-
84855460583
-
Bleach gel: A simple agarose gel for analyzing RNA quality
-
Aranda PS, Lajoie DM, Jorcyk CL. 2012. Bleach gel: a simple agarose gel for analyzing RNA quality. Electrophoresis 33, 366-369.
-
(2012)
Electrophoresis
, vol.33
, pp. 366-369
-
-
Aranda, P.S.1
Lajoie, D.M.2
Jorcyk, C.L.3
-
3
-
-
0345257783
-
Chipping away at the chip bias: RNA degradation in microarray analysis
-
Auer H, Lyianarachchi S, Newsom D, Klisovic MI, Marcucci G, Kornacker K. 2003. Chipping away at the chip bias: RNA degradation in microarray analysis. Nature Genetics 35, 292-293.
-
(2003)
Nature Genetics
, vol.35
, pp. 292-293
-
-
Auer, H.1
Lyianarachchi, S.2
Newsom, D.3
Klisovic, M.I.4
Marcucci, G.5
Kornacker, K.6
-
4
-
-
77649229497
-
MRNA and microRNA quality control for RT-qPCR analysis
-
Becker C, Hammerle-Fickinger A, Riedmaier I, Pfaffl M. 2010. mRNA and microRNA quality control for RT-qPCR analysis. Methods 50, 237-243.
-
(2010)
Methods
, vol.50
, pp. 237-243
-
-
Becker, C.1
Hammerle-Fickinger, A.2
Riedmaier, I.3
Pfaffl, M.4
-
5
-
-
77953228918
-
Expression pattern suggests a role of MiR399 in the regulation of the cellular response to local Pi increase during arbuscular mycorrhizal symbiosis
-
Branscheid A, Sieh D, Pant BD, May P, Devers EA, Elkrog A, Schauser L, Scheible W-R, Krajinski F. 2010. Expression pattern suggests a role of MiR399 in the regulation of the cellular response to local Pi increase during arbuscular mycorrhizal symbiosis. Molecular Plant-Microbe Interactions 23, 915-926.
-
(2010)
Molecular Plant-Microbe Interactions
, vol.23
, pp. 915-926
-
-
Branscheid, A.1
Sieh, D.2
Pant, B.D.3
May, P.4
Devers, E.A.5
Elkrog, A.6
Schauser, L.7
Scheible, W.-R.8
Krajinski, F.9
-
6
-
-
18344396568
-
Minimum information about a microarray experiment (MIAME)-toward standards for microarray data
-
Brazma A, Hingamp P, Quackenbush J, et al. 2001. Minimum information about a microarray experiment (MIAME)-toward standards for microarray data. Nature Genetics 29, 365-371.
-
(2001)
Nature Genetics
, vol.29
, pp. 365-371
-
-
Brazma, A.1
Hingamp, P.2
Quackenbush, J.3
-
7
-
-
0036668567
-
Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): Trends and problems
-
Bustin SA. 2002. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. Journal of Molecular Endocrinology 29, 23-39.
-
(2002)
Journal of Molecular Endocrinology
, vol.29
, pp. 23-39
-
-
Bustin, S.A.1
-
8
-
-
21344458602
-
Quantitative real-time RT-PCR-a perspective
-
Bustin SA. 2005. Quantitative real-time RT-PCR-a perspective. Journal of Molecular Endocrinology 34, 597-601.
-
(2005)
Journal of Molecular Endocrinology
, vol.34
, pp. 597-601
-
-
Bustin, S.A.1
-
9
-
-
77956673889
-
MIQE precis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative realtime PCR experiments
-
Bustin SA, Beaulieu J, Huggett J, Jaggi R, Kibenge F, Olsvik P, Penning L, Toegel S. 2010. MIQE precis: practical implementation of minimum standard guidelines for fluorescence-based quantitative realtime PCR experiments. BMC Molecular Biology 11, 74.
-
(2010)
BMC Molecular Biology
, vol.11
, pp. 74
-
-
Bustin, S.A.1
Beaulieu, J.2
Huggett, J.3
Jaggi, R.4
Kibenge, F.5
Olsvik, P.6
Penning, L.7
Toegel, S.8
-
10
-
-
64149097786
-
The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments
-
Bustin SA, Benes V, Garson J, et al. 2009. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clinical Chemistry 55, 611-622.
-
(2009)
Clinical Chemistry
, vol.55
, pp. 611-622
-
-
Bustin, S.A.1
Benes, V.2
Garson, J.3
-
11
-
-
79957717050
-
Primer sequence disclosure: A clarification of the MIQE guidelines
-
Bustin SA, Benes V, Garson JA, et al. 2011. Primer sequence disclosure: a clarification of the MIQE guidelines. Clinical Chemistry 57, 919-921.
-
(2011)
Clinical Chemistry
, vol.57
, pp. 919-921
-
-
Bustin, S.A.1
Benes, V.2
Garson, J.A.3
-
12
-
-
14044265394
-
Pitfalls of quantitative real-time reversetranscription polymerase chain reaction
-
Bustin SA, Nolan T. 2004. Pitfalls of quantitative real-time reversetranscription polymerase chain reaction. Journal of Biomolecular Techniques 15, 155-166.
-
(2004)
Journal of Biomolecular Techniques
, vol.15
, pp. 155-166
-
-
Bustin, S.A.1
Nolan, T.2
-
13
-
-
33845600784
-
Impact of RNA degradation on gene expression profiles: Assessment of different methods to reliably determine RNA quality
-
Copois V, Bibeau F, Bascoulmollevi C, Salvetat N, Chalbos P, Bareil C, Candeil L, Fraslon C, Conseiller E, Granci V. 2007. Impact of RNA degradation on gene expression profiles: assessment of different methods to reliably determine RNA quality. Journal of Biotechnology 127, 549-559.
-
(2007)
Journal of Biotechnology
, vol.127
, pp. 549-559
-
-
Copois, V.1
Bibeau, F.2
Bascoulmollevi, C.3
Salvetat, N.4
Chalbos, P.5
Bareil, C.6
Candeil, L.7
Fraslon, C.8
Conseiller, E.9
Granci, V.10
-
14
-
-
77649216508
-
Development and validation of RQI: An RNA quality indicator for the Experion™ automated electrophoresis system
-
Denisov V, Strong W, Walder M, Gringich J, Wintz H. 2008. Development and validation of RQI: an RNA quality indicator for the Experion™ automated electrophoresis system. Tech Note 5761. Bio-Rad.
-
(2008)
Tech Note 5761. Bio-Rad
-
-
Denisov, V.1
Strong, W.2
Walder, M.3
Gringich, J.4
Wintz, H.5
-
15
-
-
77649235913
-
How to do successful gene expression analysis using real-time PCR
-
Derveaux S, Vandesompele J, Hellemans J. 2010. How to do successful gene expression analysis using real-time PCR. Methods 50, 227-230.
-
(2010)
Methods
, vol.50
, pp. 227-230
-
-
Derveaux, S.1
Vandesompele, J.2
Hellemans, J.3
-
16
-
-
3142615272
-
Validation of housekeeping genes for normalizing RNA expression in real-time PCR
-
116 118-119
-
Dheda K, Huggett JF, Bustin SA, Johnson MA, Rook G, Zumla A. 2004. Validation of housekeeping genes for normalizing RNA expression in real-time PCR. BioTechniques 37, 112-114, 116, 118-119.
-
(2004)
BioTechniques
, vol.37
, pp. 112-114
-
-
Dheda, K.1
Huggett, J.F.2
Bustin, S.A.3
Johnson, M.A.4
Rook, G.5
Zumla, A.6
-
17
-
-
27644563899
-
The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization
-
Dheda K, Huggett JF, Chang JS, Kim LU, Bustin SA, Johnson MA, Rook GAW, Zumla A. 2005. The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization. Analytical Biochemistry 344, 141-143.
-
(2005)
Analytical Biochemistry
, vol.344
, pp. 141-143
-
-
Dheda, K.1
Huggett, J.F.2
Chang, J.S.3
Kim, L.U.4
Bustin, S.A.5
Johnson, M.A.6
Gaw, R.7
Zumla, A.8
-
18
-
-
80054056014
-
Characterization of the 3':5' ratio for reliable determination of RNA quality
-
Die JV, Obrero A, González-Verdejo CI, Román B. 2011. Characterization of the 3':5' ratio for reliable determination of RNA quality. Analytical Biochemistry 419, 336-338.
-
(2011)
Analytical Biochemistry
, vol.419
, pp. 336-338
-
-
Die, J.V.1
Obrero, A.2
González-Verdejo, C.I.3
Román, B.4
-
20
-
-
33644975004
-
RNA integrity and the effect on the real-time qRT-PCR performance
-
Fleige S, Pfaffl MW. 2006. RNA integrity and the effect on the real-time qRT-PCR performance. Molecular Aspects of Medicine 27, 126-139.
-
(2006)
Molecular Aspects of Medicine
, vol.27
, pp. 126-139
-
-
Fleige, S.1
Pfaffl, M.W.2
-
21
-
-
33748095919
-
Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR
-
Fleige S, Walf V, Huch S, Prgomet C, Sehm J, Pfaffl MW. 2006. Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR. Biotechnology Letters 28, 1601-1613.
-
(2006)
Biotechnology Letters
, vol.28
, pp. 1601-1613
-
-
Fleige, S.1
Walf, V.2
Huch, S.3
Prgomet, C.4
Sehm, J.5
Pfaffl, M.W.6
-
22
-
-
3242732345
-
Real-time PCR: What relevance to plant studies?
-
Gachon C. 2004. Real-time PCR: what relevance to plant studies? Journal of Experimental Botany 55, 1445-1454.
-
(2004)
Journal of Experimental Botany
, vol.55
, pp. 1445-1454
-
-
Gachon, C.1
-
23
-
-
63349098693
-
Overlapping expression patterns and differential transcript levels of phosphate transporter genes in arbuscular mycorrhizal, Pi-fertilised and phytohormone-treated Medicago truncatula roots
-
Grunwald U, Guo W, Fischer K, Isayenkov S, Ludwig-Müller J, Hause B, Yan X, Kuester H, Franken P. 2009. Overlapping expression patterns and differential transcript levels of phosphate transporter genes in arbuscular mycorrhizal, Pi-fertilised and phytohormone-treated Medicago truncatula roots. Planta 229, 1023-1034.
-
(2009)
Planta
, vol.229
, pp. 1023-1034
-
-
Grunwald, U.1
Guo, W.2
Fischer, K.3
Isayenkov, S.4
Ludwig-Müller, J.5
Hause, B.6
Yan, X.7
Kuester, H.8
Franken, P.9
-
24
-
-
67650930085
-
Normalization of qRT-PCR data: The necessity of adopting a systematic, experimental conditions-specific, validation of references
-
Guenin S, Mauriat M, Pelloux J, Van Wuytswinkel O, Bellini C, Gutierrez L. 2009. Normalization of qRT-PCR data: the necessity of adopting a systematic, experimental conditions-specific, validation of references. Journal of Experimental Botany 60, 487-493.
-
(2009)
Journal of Experimental Botany
, vol.60
, pp. 487-493
-
-
Guenin, S.1
Mauriat, M.2
Pelloux, J.3
Van Wuytswinkel, O.4
Bellini, C.5
Gutierrez, L.6
-
25
-
-
47249141242
-
The lack of a systematic validation of reference genes: A serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants
-
Gutierrez L, Mauriat M, Gunin S, et al. 2008a. The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants. Plant Biotechnology Journal 6, 609-618.
-
(2008)
Plant Biotechnology Journal
, vol.6
, pp. 609-618
-
-
Gutierrez, L.1
Mauriat, M.2
Gunin, S.3
-
26
-
-
57749113093
-
Towards a systematic validation of references in real-time RT-PCR
-
Gutierrez L, Mauriat M, Pelloux J, Bellini C, Van Wuytswinkel O. 2008b. Towards a systematic validation of references in real-time RT-PCR. The Plant Cell 20, 1734-1735.
-
(2008)
The Plant Cell
, vol.20
, pp. 1734-1735
-
-
Gutierrez, L.1
Mauriat, M.2
Pelloux, J.3
Bellini, C.4
Van Wuytswinkel, O.5
-
27
-
-
79961014660
-
Standardisation and reporting for nucleic acid quantification
-
Huggett J, Bustin SA, 2011. Standardisation and reporting for nucleic acid quantification. Accreditation and Quality Assurance 16, 399-405.
-
(2011)
Accreditation and Quality Assurance
, vol.16
, pp. 399-405
-
-
Huggett, J.1
Bustin, S.A.2
-
28
-
-
21344471138
-
Real-time RT-PCR normalisation; Strategies and considerations
-
Huggett JF, Dheda K, Bustin SA, Zumla A, 2005. Real-time RT-PCR normalisation; strategies and considerations. Genes and Immunity 6, 279-284.
-
(2005)
Genes and Immunity
, vol.6
, pp. 279-284
-
-
Huggett, J.F.1
Dheda, K.2
Bustin, S.A.3
Zumla, A.4
-
29
-
-
28644452204
-
Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces
-
Imbeaud S, Graudens E, Boulanger V, Barlet X, Zaborski P, Eveno E, Mueller O, Schroeder A, Auffray C. 2005. Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces. Nucleic Acids Research 33, e56.
-
(2005)
Nucleic Acids Research
, vol.33
-
-
Imbeaud, S.1
Graudens, E.2
Boulanger, V.3
Barlet, X.4
Zaborski, P.5
Eveno, E.6
Mueller, O.7
Schroeder, A.8
Auffray, C.9
-
31
-
-
33646135785
-
Combining laser capture microdissection with quantitative realtime PCR: Effects of tissue manipulation on RNA quality and gene expression
-
Kerman IA, Buck BJ, Evans SJ, Akil H, Watson SJ. 2006. Combining laser capture microdissection with quantitative realtime PCR: effects of tissue manipulation on RNA quality and gene expression. Journal of Neuroscience Methods 153, 71-85.
-
(2006)
Journal of Neuroscience Methods
, vol.153
, pp. 71-85
-
-
Kerman, I.A.1
Buck, B.J.2
Evans, S.J.3
Akil, H.4
Watson, S.J.5
-
32
-
-
82155186096
-
Identification of superior reference genes for data normalisation of expression studies via quantitative PCR in hybrid roses (Rosa hybrida)
-
Klie M, Debener T. 2011. Identification of superior reference genes for data normalisation of expression studies via quantitative PCR in hybrid roses (Rosa hybrida). BMC Research Notes 4, 518.
-
(2011)
BMC Research Notes
, vol.4
, pp. 518
-
-
Klie, M.1
Debener, T.2
-
33
-
-
35348928033
-
Laser capture microdissection (LCM) and expression analyses of Glycine max (Soybean) syncytium containing root regions formed by the plant pathogen Heterodera glycines (Soybean Cyst Nematode)
-
Klink VP, Alkharouf NW, Macdonald M, Matthews B. 2007. Laser capture microdissection (LCM) and expression analyses of Glycine max (Soybean) syncytium containing root regions formed by the plant pathogen Heterodera glycines (Soybean Cyst Nematode). Planta 226, 1389-1409.
-
(2007)
Planta
, vol.226
, pp. 1389-1409
-
-
Klink, V.P.1
Alkharouf, N.W.2
MacDonald, M.3
Matthews, B.4
-
34
-
-
79960226202
-
RNA integrity in post-mortem samples: Influencing parameters and implications on RT-qPCR assays
-
Koppelkamm A, Vennemann B, Lutz-Bonengel S, Fracasso T, Vennemann M. 2011. RNA integrity in post-mortem samples: influencing parameters and implications on RT-qPCR assays. International Journal of Legal Medicine 125, 573-580.
-
(2011)
International Journal of Legal Medicine
, vol.125
, pp. 573-580
-
-
Koppelkamm, A.1
Vennemann, B.2
Lutz-Bonengel, S.3
Fracasso, T.4
Vennemann, M.5
-
35
-
-
23444456437
-
Effects of RNA degradation on gene expression analysis of human postmortem tissues
-
Lee J, Hever A, Willhite D, Zlotnik A, Hevezi P. 2005. Effects of RNA degradation on gene expression analysis of human postmortem tissues. FASEB Journal 19, 1356-1358.
-
(2005)
FASEB Journal
, vol.19
, pp. 1356-1358
-
-
Lee, J.1
Hever, A.2
Willhite, D.3
Zlotnik, A.4
Hevezi, P.5
-
36
-
-
40049086211
-
Improved RNA quality and TaqMan® Pre-amplification method (PreAmp) to enhance expression analysis from formalin fixed paraffin embedded (FFPE) materials
-
Li J, Smyth P, Cahill S, Denning K, Flavin R, Aherne S, Pirotta M, Guenther SM, O'Leary JJ, Sheils O. 2008. Improved RNA quality and TaqMan® Pre-amplification method (PreAmp) to enhance expression analysis from formalin fixed paraffin embedded (FFPE) materials. BMC Biotechnology 8, 10.
-
(2008)
BMC Biotechnology
, vol.8
, pp. 10
-
-
Li, J.1
Smyth, P.2
Cahill, S.3
Denning, K.4
Flavin, R.5
Aherne, S.6
Pirotta, M.7
Guenther, S.M.8
O'Leary, J.J.9
Sheils, O.10
-
37
-
-
63449111012
-
Assessing the quality of hybridized RNA in Affymetrix GeneChips using linear regression
-
Li M, Reilly C. 2008. Assessing the quality of hybridized RNA in Affymetrix GeneChips using linear regression. Journal of Biomolecular Techniques 19, 122-128.
-
(2008)
Journal of Biomolecular Techniques
, vol.19
, pp. 122-128
-
-
Li, M.1
Reilly, C.2
-
38
-
-
79951521045
-
Identification and validation of reference genes for normalization of transcripts from virus-infected Arabidopsis thaliana
-
Lilly S, Drummond R, Pearson M, MacDiarmid R. 2011. Identification and validation of reference genes for normalization of transcripts from virus-infected Arabidopsis thaliana. Molecular Plant-Microbe Interactions 24, 294-304.
-
(2011)
Molecular Plant-Microbe Interactions
, vol.24
, pp. 294-304
-
-
Lilly, S.1
Drummond, R.2
Pearson, M.3
MacDiarmid, R.4
-
39
-
-
33748745764
-
Critical factors in gene expression in postmortem human brain: Focus on studies in schizophrenia
-
Lipska BK, Deep-Soboslay A, Weickert CS, Hyde TM, Martin CE, Herman MM, Kleinman JE. 2006. Critical factors in gene expression in postmortem human brain: focus on studies in schizophrenia. Biological Psychiatry 60, 650-658.
-
(2006)
Biological Psychiatry
, vol.60
, pp. 650-658
-
-
Lipska, B.K.1
Deep-Soboslay, A.2
Weickert, C.S.3
Hyde, T.M.4
Martin, C.E.5
Herman, M.M.6
Kleinman, J.E.7
-
40
-
-
0028990826
-
Value of A260/A280 ratios for measurement of purity of nucleic acids
-
Manchester KL. 1995. Value of A260/A280 ratios for measurement of purity of nucleic acids. BioTechniques 19, 208-210.
-
(1995)
BioTechniques
, vol.19
, pp. 208-210
-
-
Manchester, K.L.1
-
41
-
-
0030004766
-
Use of UV methods for measurement of protein and nucleic acid concentrations
-
Manchester KL. 1996. Use of UV methods for measurement of protein and nucleic acid concentrations. BioTechniques 20, 968-970.
-
(1996)
BioTechniques
, vol.20
, pp. 968-970
-
-
Manchester, K.L.1
-
42
-
-
9944244481
-
Denaturing RNA electrophoresis in TAE agarose gels
-
Masek T, Vopalensky V, Suchomelova P, Pospisek M. 2005. Denaturing RNA electrophoresis in TAE agarose gels. Analytical Biochemistry 336, 46-50.
-
(2005)
Analytical Biochemistry
, vol.336
, pp. 46-50
-
-
Masek, T.1
Vopalensky, V.2
Suchomelova, P.3
Pospisek, M.4
-
43
-
-
0033986177
-
A microfluidic system for high-speed reproducible DNA sizing and quantitation
-
Mueller O, Hahnenberger K, Dittmann M, Yee H, Dubrow R, Nagle R, Ilsley D. 2000. A microfluidic system for high-speed reproducible DNA sizing and quantitation. Electrophoresis 21, 128-134.
-
(2000)
Electrophoresis
, vol.21
, pp. 128-134
-
-
Mueller, O.1
Hahnenberger, K.2
Dittmann, M.3
Yee, H.4
Dubrow, R.5
Nagle, R.6
Ilsley, D.7
-
44
-
-
84868019873
-
Procedures for quality control of RNA samples for use in quantitative reverse transcription PCR
-
Keer JT, Birch L, eds London: The Royal Society of Chemistry
-
Nolan T, Bustin S. 2008. Procedures for quality control of RNA samples for use in quantitative reverse transcription PCR. In: Keer JT, Birch L, eds. Essentials of nucleic acid analysis: a robust approach. London: The Royal Society of Chemistry, 189-207.
-
(2008)
Essentials of Nucleic Acid Analysis: A Robust Approach
, pp. 189-207
-
-
Nolan, T.1
Bustin, S.2
-
45
-
-
33847643776
-
Quantification of mRNA using real-time RT-PCR
-
Nolan T, Hands RE, Bustin SA. 2006b. Quantification of mRNA using real-time RT-PCR. Nature Protocols 1, 1559-1582.
-
(2006)
Nature Protocols
, vol.1
, pp. 1559-1582
-
-
Nolan, T.1
Hands, R.E.2
Bustin, S.A.3
-
46
-
-
33645293441
-
SPUD: A quantitative PCR assay for the detection of inhibitors in nucleic acid preparations
-
Nolan T, Hands R, Ogunkolade W, Bustin SA. 2006a. SPUD: a quantitative PCR assay for the detection of inhibitors in nucleic acid preparations. Analytical Biochemistry 351, 308-310.
-
(2006)
Analytical Biochemistry
, vol.351
, pp. 308-310
-
-
Nolan, T.1
Hands, R.2
Ogunkolade, W.3
Bustin, S.A.4
-
47
-
-
77955290840
-
Impact of RNA degradation on gene expression profiling
-
Opitz L, Salinas-Riester G, Grade M, Jung K, Jo P, Emons G, Ghadimi BM, Beißbarth T, Gaedcke J. 2010. Impact of RNA degradation on gene expression profiling. BMC Medical Genomics 3, 36.
-
(2010)
BMC Medical Genomics
, vol.3
, pp. 36
-
-
Opitz, L.1
Salinas-Riester, G.2
Grade, M.3
Jung, K.4
Jo, P.5
Emons, G.6
Ghadimi, B.M.7
Beißbarth, T.8
Gaedcke, J.9
-
48
-
-
24944584586
-
Impact of RNA quality on reference gene expression stability
-
54, 56
-
Pérez-Novo CA, Claeys C, Speleman F, Van Cauwenberge P, Bachert C, Vandesompele J. 2005. Impact of RNA quality on reference gene expression stability. BioTechniques 39, 52, 54, 56.
-
(2005)
BioTechniques
, vol.39
, pp. 52
-
-
Pérez-Novo, C.A.1
Claeys, C.2
Speleman, F.3
Van Cauwenberge, P.4
Bachert, C.5
Vandesompele, J.6
-
49
-
-
77649227839
-
The ongoing evolution of qPCR
-
Pfaffl MW. 2010. The ongoing evolution of qPCR. Methods 50, 215-216.
-
(2010)
Methods
, vol.50
, pp. 215-216
-
-
Pfaffl, M.W.1
-
50
-
-
51849106834
-
Validation of lab-on-chip capillary electrophoresis systems for total RNA quality and quantity control
-
Pfaffl MW, Fleige S, Riedmaier I. 2008. Validation of lab-on-chip capillary electrophoresis systems for total RNA quality and quantity control. Biotechnology and Biotechnological Equipment 22, 839-834.
-
(2008)
Biotechnology and Biotechnological Equipment
, vol.22
, pp. 839-834
-
-
Pfaffl, M.W.1
Fleige, S.2
Riedmaier, I.3
-
51
-
-
84859347775
-
Selection of suitable reference genes for RT-qPCR analyses in cyanobacteria
-
Pinto F, Pacheco CC, Ferreira D, Moradas-Ferreira P, Tamagnini P. 2012. Selection of suitable reference genes for RT-qPCR analyses in cyanobacteria. PLoS ONE 7, e34983.
-
(2012)
PLoS ONE
, vol.7
-
-
Pinto, F.1
Pacheco, C.C.2
Ferreira, D.3
Moradas-Ferreira, P.4
Tamagnini, P.5
-
52
-
-
0346728757
-
Guideline to reference gene selection for quantitative real-time PCR
-
Radoni A, Thulke S, Mackay IM, Landt O, Siegert W, Nitsche A. 2004. Guideline to reference gene selection for quantitative real-time PCR. Biochemical and Biophysical Research Communications 313, 856-862.
-
(2004)
Biochemical and Biophysical Research Communications
, vol.313
, pp. 856-862
-
-
Radoni, A.1
Thulke, S.2
MacKay, I.M.3
Landt, O.4
Siegert, W.5
Nitsche, A.6
-
53
-
-
77955393165
-
Experimental comparison of relative RT-qPCR quantification approaches for gene expression studies in poplar
-
Regier N, Frey B. 2010. Experimental comparison of relative RT-qPCR quantification approaches for gene expression studies in poplar. BMC Molecular Biology 11, 57.
-
(2010)
BMC Molecular Biology
, vol.11
, pp. 57
-
-
Regier, N.1
Frey, B.2
-
55
-
-
0001760677
-
Molecular cloning: A laboratory manual
-
NY: Cold Spring Harbor Laboratory Press
-
Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
-
(1989)
Cold Spring Harbor
-
-
Sambrook, J.1
Fritsch, E.F.2
Maniatis, T.3
-
56
-
-
0345168051
-
Moderate degradation does not preclude microarray analysis of small amounts of RNA
-
1198-1201
-
Schoor O, Weinschenk T, Hennenlotter J, Corvin S, Stenzl A, Rammensee H-G, Stevanovi? S. 2003. Moderate degradation does not preclude microarray analysis of small amounts of RNA. BioTechniques 35, 1192-1196, 1198-1201.
-
(2003)
BioTechniques
, vol.35
, pp. 1192-1196
-
-
Schoor, O.1
Weinschenk, T.2
Hennenlotter, J.3
Corvin, S.4
Stenzl, A.5
Rammensee, H.-G.6
Stevanovi, S.7
-
57
-
-
33645241407
-
The RIN: An RNA integrity number for assigning integrity values to RNA measurements
-
Schroeder A, Mueller O, Stocker S, Salowsky R, Leiber M, Gassmann M, Lightfoot S, Menzel W, Granzow M, Ragg T. 2006. The RIN: an RNA integrity number for assigning integrity values to RNA measurements. BMC Molecular Biology 7, 3.
-
(2006)
BMC Molecular Biology
, vol.7
, pp. 3
-
-
Schroeder, A.1
Mueller, O.2
Stocker, S.3
Salowsky, R.4
Leiber, M.5
Gassmann, M.6
Lightfoot, S.7
Menzel, W.8
Granzow, M.9
Ragg, T.10
-
58
-
-
34250308050
-
RNA quality in frozen breast cancer samples and the influence on gene expression analysis-comparison of three evaluation methods using microcapillary electrophoresis traces
-
Strand C, Enell J, Hedenfalk I, Fernö M. 2007. RNA quality in frozen breast cancer samples and the influence on gene expression analysis-comparison of three evaluation methods using microcapillary electrophoresis traces. BMC Molecular Biology 8, 38.
-
(2007)
BMC Molecular Biology
, vol.8
, pp. 38
-
-
Strand, C.1
Enell, J.2
Hedenfalk, I.3
Fernö, M.4
-
59
-
-
0034012360
-
Assessment of RNA quality by semi-quantitative RT-PCR of multiple regions of a long ubiquitous mRNA
-
Swift GH, Peyton M, MacDonald R. 2000. Assessment of RNA quality by semi-quantitative RT-PCR of multiple regions of a long ubiquitous mRNA. BioTechniques 28, 524-531.
-
(2000)
BioTechniques
, vol.28
, pp. 524-531
-
-
Swift, G.H.1
Peyton, M.2
MacDonald, R.3
-
60
-
-
84868029269
-
A MIQE case study-effect of RNA sample quality and reference gene stability on gene expression data
-
Bio-Rad
-
Taylor S. 2011. A MIQE case study-effect of RNA sample quality and reference gene stability on gene expression data. Tech Note 6245, 1-6. Bio-Rad.
-
(2011)
Tech Note
, vol.6245
, pp. 1-6
-
-
Taylor, S.1
-
61
-
-
77649212939
-
A practical approach to RT-qPCR-publishing data that conform to the MIQE guidelines
-
Taylor S, Wakem M, Dijkman G, Alsarraj M, Nguyen M. 2010. A practical approach to RT-qPCR-publishing data that conform to the MIQE guidelines. Methods 50, S1-S5.
-
(2010)
Methods
, vol.50
-
-
Taylor, S.1
Wakem, M.2
Dijkman, G.3
Alsarraj, M.4
Nguyen, M.5
-
62
-
-
33745129067
-
Effect of acute aldosterone administration on gene expression profile in the heart
-
Turchin A. 2006. Effect of acute aldosterone administration on gene expression profile in the heart. Endocrinology 147, 3183-3189.
-
(2006)
Endocrinology
, vol.147
, pp. 3183-3189
-
-
Turchin, A.1
-
64
-
-
67649444944
-
Reference gene validation software for improved normalization
-
Logan J, Edwards K Saunders N, eds Norfolk: Caister Academic Press
-
Vandesompele J, Kubista M, Pfaffl MW. 2009. Reference gene validation software for improved normalization. In: Logan J, Edwards K, Saunders N, eds. Real-time PCR: current technology and applications. Norfolk: Caister Academic Press, 47-64.
-
(2009)
Real-time PCR: Current Technology and Applications
, pp. 47-64
-
-
Vandesompele, J.1
Kubista, M.2
Pfaffl, M.W.3
-
65
-
-
79955999748
-
Measurable impact of RNA quality on gene expression results from quantitative PCR
-
Vermeulen J, De Preter K, Lefever S, Nuytens J, De Vloed F, Derveaux S, Hellemans J, Speleman F, Vandesompele J. 2011. Measurable impact of RNA quality on gene expression results from quantitative PCR. Nucleic Acids Research 39, e63.
-
(2011)
Nucleic Acids Research
, vol.39
-
-
Vermeulen, J.1
De Preter, K.2
Lefever, S.3
Nuytens, J.4
De Vloed, F.5
Derveaux, S.6
Hellemans, J.7
Speleman, F.8
Vandesompele, J.9
|