메뉴 건너뛰기




Volumn 39, Issue 9, 2011, Pages

Measurable impact of RNA quality on gene expression results from quantitative PCR

Author keywords

[No Author keywords available]

Indexed keywords

HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; MESSENGER RNA; RNA; RNA 18S; RNA 28S; TRANSFER RNA; UBIQUITIN CONJUGATING ENZYME;

EID: 79955999748     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkr065     Document Type: Article
Times cited : (143)

References (27)
  • 2
    • 0033775615 scopus 로고    scopus 로고
    • Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
    • Bustin, S.A. (2000) Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol., 25, 169-193.
    • (2000) J. Mol. Endocrinol. , vol.25 , pp. 169-193
    • Bustin, S.A.1
  • 3
    • 0036278859 scopus 로고    scopus 로고
    • Gene quantification using real-time quantitative PCR: An emerging technology hits the mainstream
    • DOI 10.1016/S0301-472X(02)00806-8, PII S0301472X02008068
    • Ginzinger, D.G. (2002) Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Exp. Hematol., 30, 503-512. (Pubitemid 34626648)
    • (2002) Experimental Hematology , vol.30 , Issue.6 , pp. 503-512
    • Ginzinger, D.G.1
  • 4
    • 66749160449 scopus 로고    scopus 로고
    • Real-time quantitative PCR - Opportunities and pitfalls
    • Bustin, S.A. (2008) Real-time quantitative PCR - opportunities and pitfalls. Eur. Pharm. Rev., 4, 18-23.
    • (2008) Eur. Pharm. Rev. , vol.4 , pp. 18-23
    • Bustin, S.A.1
  • 5
    • 66549129231 scopus 로고    scopus 로고
    • Reliability of real-time reverse-transcription PCR in clinical diagnostics: Gold standard or substandard?
    • Murphy, J. and Bustin, S.A. (2009) Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold standard or substandard? Expert Rev. Mol. Diagn., 9, 187-197.
    • (2009) Expert Rev. Mol. Diagn. , vol.9 , pp. 187-197
    • Murphy, J.1    Bustin, S.A.2
  • 8
    • 33644975004 scopus 로고    scopus 로고
    • RNA integrity and the effect on the real-time qRT-PCR performance
    • Fleige, S. and Pfaffl, M.W. (2006) RNA integrity and the effect on the real-time qRT-PCR performance. Mol. Aspects Med., 27, 126-139.
    • (2006) Mol. Aspects Med. , vol.27 , pp. 126-139
    • Fleige, S.1    Pfaffl, M.W.2
  • 9
    • 33645293441 scopus 로고    scopus 로고
    • SPUD: A quantitative PCR assay for the detection of inhibitors in nucleic acid preparations
    • Nolan, T., Hands, R.E., Ogunkolade, W. and Bustin, S.A. (2006) SPUD: a quantitative PCR assay for the detection of inhibitors in nucleic acid preparations. Anal. Biochem., 351, 308-310.
    • (2006) Anal. Biochem. , vol.351 , pp. 308-310
    • Nolan, T.1    Hands, R.E.2    Ogunkolade, W.3    Bustin, S.A.4
  • 10
    • 0038012549 scopus 로고    scopus 로고
    • Biological sample collection and processing for molecular epidemiological studies
    • DOI 10.1016/S1383-5742(02)00090-X, PII S138357420200090X
    • Holland, N.T., Smith, M.T., Eskenazi, B. and Bastaki, M. (2003) Biological sample collection and processing for molecular epidemiological studies. Mutat. Res., 543, 217-234. (Pubitemid 36645081)
    • (2003) Mutation Research - Reviews in Mutation Research , vol.543 , Issue.3 , pp. 217-234
    • Holland, N.T.1    Smith, M.T.2    Eskenazi, B.3    Bastaki, M.4
  • 12
    • 33847643776 scopus 로고    scopus 로고
    • Quantification of mRNA using real-time RT-PCR
    • Nolan, T., Hands, R.E. and Bustin, S.A. (2006) Quantification of mRNA using real-time RT-PCR. Nat. Protoc., 1, 1559-1582.
    • (2006) Nat. Protoc. , vol.1 , pp. 1559-1582
    • Nolan, T.1    Hands, R.E.2    Bustin, S.A.3
  • 16
    • 25444523728 scopus 로고    scopus 로고
    • Novel isothermal, linear nucleic acid amplification systems for highly multiplexed applications
    • DOI 10.1373/clinchem.2005.053694
    • Kurn, N., Chen, P., Heath, J.D., Kopf-Sill, A., Stephens, K.M. and Wang, S. (2005) Novel isothermal, linear nucleic acid amplification systems for highly multiplexed applications. Clin. Chem., 51, 1973-1981. (Pubitemid 41368197)
    • (2005) Clinical Chemistry , vol.51 , Issue.10 , pp. 1973-1981
    • Kurn, N.1    Chen, P.2    Heath, J.D.3    Kopf-Sill, A.4    Stephens, K.M.5    Wang, S.6
  • 20
    • 0037129827 scopus 로고    scopus 로고
    • Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    • Research00341-Research003411
    • Vandesompele, J., De Preter, K., Pattyn, F., Poppe, B., Van Roy, N., De Paepe, A. and Speleman, F. (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol., 3, Research0034.1- Research0034.11.
    • (2002) Genome Biol. , vol.3
    • Vandesompele, J.1    De Preter, K.2    Pattyn, F.3    Poppe, B.4    Van Roy, N.5    De Paepe, A.6    Speleman, F.7
  • 21
    • 45449087921 scopus 로고    scopus 로고
    • QBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data
    • Hellemans, J., Mortier, G., De Paepe, A., Speleman, F. and Vandesompele, J. (2007) qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biol., 8, R19.
    • (2007) Genome Biol. , vol.8
    • Hellemans, J.1    Mortier, G.2    De Paepe, A.3    Speleman, F.4    Vandesompele, J.5
  • 24
    • 18944393902 scopus 로고    scopus 로고
    • A compendium to ensure computational reproducibility in high-dimensional classification tasks
    • Article37
    • Ruschhaupt, M., Huber, W., Poustka, A. and Mansmann, U. (2004) A compendium to ensure computational reproducibility in high-dimensional classification tasks. Stat. Appl. Genet. Mol. Biol., 3, Article37.
    • (2004) Stat. Appl. Genet. Mol. Biol. , vol.3
    • Ruschhaupt, M.1    Huber, W.2    Poustka, A.3    Mansmann, U.4
  • 26
    • 27144468030 scopus 로고    scopus 로고
    • Reliable gene expression measurements from degraded RNA by quantitative real-time PCR depend on short amplicons and a proper normalization
    • DOI 10.1038/labinvest.3700303, PII 3700303
    • Antonov, J., Goldstein, D.R., Oberli, A., Baltzer, A., Pirotta, M., Fleischmann, A., Altermatt, H.J. and Jaggi, R. (2005) Reliable gene expression measurements from degraded RNA by quantitative real-time PCR depend on short amplicons and a proper normalization. Lab Invest., 85, 1040-1050. (Pubitemid 41486428)
    • (2005) Laboratory Investigation , vol.85 , Issue.8 , pp. 1040-1050
    • Antonov, J.1    Goldstein, D.R.2    Oberli, A.3    Baltzer, A.4    Pirotta, M.5    Fleischmann, A.6    Altermatt, H.J.7    Jaggi, R.8
  • 27
    • 33745482592 scopus 로고    scopus 로고
    • Reverse transcription using random pentadecamer primers increases yield and quality of resulting cDNA
    • DOI 10.2144/000112153
    • Stangegaard, M., Dufva, I.H. and Dufva, M. (2006) Reverse transcription using random pentadecamer primers increases yield and quality of resulting cDNA. Biotechniques, 40, 649-657. (Pubitemid 46542615)
    • (2006) BioTechniques , vol.40 , Issue.5 , pp. 649-657
    • Stangegaard, M.1    Dufva, I.H.2    Dufva, M.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.