mRNA and microRNA quality control for RT-qPCR analysis

Methods

Volumn 50, Issue 4, 2010, Pages 237-243

  Becker, C ^a   Hammerle Fickinger, A ^a   Riedmaier, I ^a   Pfaffl, M W ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Gene expression; miRNA; mRNA; Normalization; RNA quality; RT qPCR

Indexed keywords

MESSENGER RNA; MICRORNA;

ARTICLE; COMPARATIVE STUDY; FRAGMENTATION REACTION; GENE EXPRESSION PROFILING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUALITY CONTROL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA DEGRADATION;

ANIMALS; CATTLE; DNA PRIMERS; GENE EXPRESSION PROFILING; HUMANS; LAB-ON-A-CHIP DEVICES; LEUKOCYTES; MICRORNAS; QUALITY CONTROL; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; RNA, MESSENGER; ULTRAVIOLET RAYS;

EID: 77649229497     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2010.01.010     Document Type: Article

References (19)

1. Fleige S., Walf V., Huch S., Prgomet C., Sehm J., and Pfaffl M.W. Biotechnol. Lett. 28 (2006) 1601-1613
2. Fleige S., and Pfaffl M.W. Mol. Aspects Med. 27 (2006) 126-139
3. Sarkar D., Parkin R., Wyman S., Bendoraite A., Sather C., Delrow J., Godwin A.K., Drescher C., Huber W., Gentleman R., and Tewari M. Nucleic Acids Res. 37 (2009) e17
4. Bustin S.A., Benes V., Garson J.A., Hellemans J., Huggett J., Kubista M., Mueller R., Nolan T., Pfaffl M.W., Shipley G.L., Vandesompele J., and Wittwer C.T. Clin. Chem. 55 (2009) 611-622
5. Manchester K.L. Biotechniques 20 (1996) 968-970
6. Gallagher S.R., and Desjardins P.R. Curr. Protoc. Mol. Biol. 3 (2006) (Appendix)
7. Schroeder A., Mueller O., Stocker S., Salowsky R., Leiber M., Gassmann M., Lightfoot S., Menzel W., Granzow M., and Ragg T. BMC Mol. Biol. 7 (2006) 3
8. Lee R.C., Feinbaum R.L., and Ambros V. Cell 75 (1993) 843-854
9. Esquela-Kerscher A., and Slack F.J. Nat. Rev. Cancer 6 (2006) 259-269
10. Lu J., Getz G., Miska E.A., varez-Saavedra E., Lamb J., Peck D., Sweet-Cordero A., Ebert B.L., Mak R.H., Ferrando A.A., Downing J.R., Jacks T., Horvitz H.R., and Golub T.R. Nature 435 (2005) 834-838
11. Mitchell P.S., Parkin R.K., Kroh E.M., Fritz B.R., Wyman S.K., Pogosova-Agadjanyan E.L., Peterson A., Noteboom J., O'Briant K.C., Allen A., Lin D.W., Urban N., Drescher C.W., Knudsen B.S., Stirewalt D.L., Gentleman R., Vessella R.L., Nelson P.S., Martin D.B., and Tewari M. Proc. Natl. Acad. Sci. USA 105 (2008) 10513-10518
12. Nolan T., Hands R.E., and Bustin S.A. Nat. Protoc. 1 (2006) 1559-1582
13. Ibberson D., Benes V., Muckenthaler M.U., and Castoldi M. BMC Biotechnol. 9 (2009) 102
14. Livak K.J., and Schmittgen T.D. Methods 25 (2001) 402-408
15. Vandesompele J., De P.K., Pattyn F., Poppe B., Van R.N., De P.A., and Speleman F. Genome Biol. 3 (2002) (RESEARCH0034)
16. Ambros V. Cell 107 (2001) 823-826
17. Peltier H.J., and Latham G.J. RNA 14 (2008) 844-852
18. Mestdagh P., Van V.P., De W.A., Muth D., Westermann F., Speleman F., and Vandesompele J. Genome Biol. 10 (2009) R64
19. I. Riedmaier, M. Bergmaier, M.W. Pfaffl, J. Nucleic Acids, submitted for publication.