Use of UV methods for measurement of protein and nucleic acid concentrations

BioTechniques

Volumn 20, Issue 6, 1996, Pages 968-970

  Manchester, Keith L ^a  

^a UNIVERSITY OF THE WITWATERSRAND   (South Africa)

Author keywords

[No Author keywords available]

Indexed keywords

ABSORPTION; ANALYTIC METHOD; ARTICLE; COLORIMETRY; DNA CONTENT; NUCLEIC ACID ANALYSIS; PROTEIN ANALYSIS; ULTRAVIOLET RADIATION;

DNA; MODELS, THEORETICAL; PROTEINS; RNA; SENSITIVITY AND SPECIFICITY; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 0030004766     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96206bm05     Document Type: Article

References (12)

1. Dunn, M.J. 1989. Determination of total protein concentration, p. 10-18. In E.L.V. Harris and S. Angal (Eds.), Protein Purification Methods. IRL Press, Oxford.
2. 1% values for protein at selected wavelengths of the ultraviolet and visible region, p. 196-327. Practical Handbook of Biochemistry and Molecular Biology. CRC Press, Boca Raton.
3. Groves, W.E., F.C. Davis and B.H. Sells. 1968. Spectrophotometric determination of microgram quantities of protein without nucleic acid interference. Anal. Biochem. 22:195-210.
4. Harlow, E. and D. Lane. 1988. Protein quantitation - UV detection, p. 673. Antibodies: A Laboratory Manual. Academic Press, New York.
5. Layne, E. 1957. Spectrophotometric and turbidimetric methods for measuring proteins. Methods Enzymol. 3:447-454.
6. 280 ratios for measurement of purity of nucleic acids. BioTechniques 19:208-210.
7. Munro, H.N. and A. Fleck. 1969. Analysis of nitrogenous constitutents, p. 423-525. In H.N. Munro (Ed.), Mammalian Protein Metabolism, Vol. 3. Academic Press, New York.
8. Peterson, G.L. 1983. Determination of total protein. Methods Enzymol. 91:95-119.
9. Scopes, R.K. 1974. Measurement of protein by spectrophotometry. Anal. Biochem. 59:277-282.
10. Smith, J.A. 1987. Quantitation of proteins, p. 10.1.1-3. In F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl (Eds.), Current Protocols in Molecular Biology. John Wiley, New York.
11. Waddell, W.J. 1956. A simple ultraviolet spectrophotometric method for the determination of protein. J. Lab. Clin. Med. 48:311-314.
12. Warburg, O. and W. Christian. 1942. Isolation and crystallization of enolase. Biochem. Z. 310:384-421.