Primer sequence disclosure: A clarification of the MIQE guidelines

Clinical Chemistry

Volumn 57, Issue 6, 2011, Pages 919-921

  Bustin, Stephen A ^a   Benes, Vladimir ^b   Garson, Jeremy A ^c   Hellemans, Jan ^d,e   Huggett, Jim ^f,g   Kubista, Mikael ^h,i   Mueller, Reinhold ^j   Nolan, Tania ^k   Pfaffl, Michael W ^l   Shipley, Gregory L ^m   Vandesompele, Jo ^d,e   Wittwer, Carl T ^n,o  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^c ROYAL FREE AND UNIVERSITY COLLEGE MEDICAL SCHOOL   (United Kingdom)

^d GHENT UNIVERSITY HOSPITAL   (Belgium)

^e Biogazelle NV   (Belgium)

^f LGC   (United Kingdom)

^g UNIVERSITY COLLEGE LONDON   (United Kingdom)

^h TATAA Biocenter   (Sweden)

ⁱ INSTITUTE OF BIOTECHNOLOGY   (Czech Republic)

^j SEQUENOM INC   (United States)

^k Sigma Aldrich   (United Kingdom)

^l TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^m UNIVERSITY OF TEXAS   (United States)

ⁿ UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

^o ARUP INSTITUTE FOR CLINICAL AND EXPERIMENTAL PATHOLOGY   (United States)

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Author keywords

[No Author keywords available]

Indexed keywords

AMPLICON; INSTRUMENT VALIDATION; LETTER; NUCLEIC ACID PROBE; PRACTICE GUIDELINE; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS;

BASE SEQUENCE; DNA PRIMERS; GUIDELINES AS TOPIC; POLYMERASE CHAIN REACTION;

EID: 79957717050     PISSN: 00099147     EISSN: 15308561     Source Type: Journal    
DOI: 10.1373/clinchem.2011.162958     Document Type: Letter

References (2)

1. Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 2009;55:611-22.
2. Bustin SA, Beaulieu JF, Huggett J, Jaggi R, Kibenge FS, Olsvik PA, et al. MIQE précis: practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments. BMC Mol Biol 2010;11:74.