Reliable gene expression measurements from degraded RNA by quantitative real-time PCR depend on short amplicons and a proper normalization

Laboratory Investigation

Volumn 85, Issue 8, 2005, Pages 1040-1050

  Antonov, Janine ^a   Goldstein, Darlene R ^b   Oberli, Andrea ^a   Baltzer, Anna ^a   Pirotta, Marco ^c   Fleischmann, Achim ^a   Altermatt, Hans J ^d   Jaggi, Rolf ^a  

^a UNIVERSITY OF BERN   (Switzerland)

^b EPFL   (Switzerland)

^c Applied Biosystems   (Switzerland)

^d Pathology Langgasse   (Switzerland)

Author keywords

Breast cancer diagnosis; Formalin fixed; Paraffin embedded material; Quantitative real time PCR

Indexed keywords

RNA;

AMPLICON; ARTICLE; BREAST CARCINOMA; CONTROLLED STUDY; ENZYME DEGRADATION; GENE CONTROL; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN TISSUE; MATHEMATICAL COMPUTING; MOLECULAR MODEL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS;

BASE SEQUENCE; BREAST NEOPLASMS; CELL LINE, TUMOR; DNA PRIMERS; DNA PROBES; FEMALE; GENE EXPRESSION PROFILING; HUMANS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, NEOPLASM;

EID: 27144468030     PISSN: 00236837     EISSN: 15300307     Source Type: Journal    
DOI: 10.1038/labinvest.3700303     Document Type: Article

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