MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

BMC Molecular Biology

Volumn 11, Issue , 2010, Pages

  Bustin, Stephen A ^a   Beaulieu, Jean François ^b   Huggett, Jim ^c   Jaggi, Rolf ^d   Kibenge, Frederick S B ^e   Olsvik, Pål A ^f   Penning, Louis C ^g   Toegel, Stefan ^h  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^b UNIVERSITÉ DE SHERBROOKE   (Canada)

^c LGC   (United Kingdom)

^d UNIVERSITY OF BERN   (Switzerland)

^e UNIVERSITY OF PRINCE EDWARD ISLAND   (Canada)

^f INSTITUTE OF MARINE RESEARCH   (Norway)

^g UTRECHT UNIVERSITY   (Netherlands)

^h UNIVERSITY OF VIENNA   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

MICRORNA;

ASSAY; DATA ANALYSIS; DNA TEMPLATE; EDITORIAL; FLUORESCENCE ANALYSIS; GENE EXPRESSION PROFILING; GENE IDENTIFICATION; PRACTICE GUIDELINE; PUBLICATION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REFERENCE DATABASE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VALIDATION STUDY; FLUORESCENCE; INSTRUMENTATION; METHODOLOGY; PEER REVIEW; POLYMERASE CHAIN REACTION; PUBLISHING; STANDARD;

PRECIS;

FLUORESCENCE; PEER REVIEW, RESEARCH; PERIODICALS AS TOPIC; POLYMERASE CHAIN REACTION; PUBLISHING;

EID: 77956673889     PISSN: None     EISSN: 14712199     Source Type: Journal    
DOI: 10.1186/1471-2199-11-74     Document Type: Editorial

References (3)

1. Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 2009, 55(4):611-622. 10.1373/clinchem.2008.112797, 19246619.
2. Nolan T, Hands RE, Bustin SA. Quantification of mRNA using real-time RT-PCR. Nature Protocols 2006, 1(3):1559-1582. 10.1038/nprot.2006.236, 17406449.
3. Derveaux S, Vandesompele J, Hellemans J. How to do successful gene expression analysis using real-time PCR. Methods 2010, 50(4):227-230. 10.1016/j.ymeth.2009.11.001, 19969088.