Assessment of RNA quality by semi-quantitative RT-PCR of multiple regions of a long ubiquitous mRNA

BioTechniques

Volumn 28, Issue 3, 2000, Pages 524-531

  Swift, Galvin H ^a   Peyton, Michael J ^a   MacDonald, Raymond J ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYTIC METHOD; ARTICLE; CONTROLLED STUDY; GENE AMPLIFICATION; GENE ISOLATION; NUCLEOTIDE SEQUENCE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA CLEAVAGE; RNA DEGRADATION;

EID: 0034012360     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00283rr01     Document Type: Article

References (8)

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2. Chirala, C.S., R. Kasturi, M. Pazirandeh, D.T. Stolow, W.-Y. Huang and S.J. Wakil. 1989. A novel cDNA extension procedure. J. Biol. Chem. 264:3750-3757.
3. Chomczynski, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
4. Hamburger, V. and H.L. Hamilton. 1951. A series of normal stages in the development of the chick embryo. J. Morph. 88:49-92.
5. Holzer, K.P., W. Liu and G.G. Hammes. 1989. Molecular cloning and sequencing of chicken liver fatty acid synthase cDNA. Proc. Natl. Acad. Sci. USA 86:4387-4391.
6. Semenkovich, C.F., T. Coleman and F.T.J. Fiedorek. 1995. Human fatty acid synthase mRNA: tissue distribution, genetic mapping, and kinetics of decay after glucose deprivation. J. Lipid Res. 36:1507-1521.
7. Watzinger, F. and T. Lion. 1998. Multiplex PCR for quality control of template RNA/cDNA in RT-PCR assays. Leukemia 12:1984-1986.
8. Yuan, Z., W. Liu and G.G. Hammes. 1988. Molecular cloning and sequencing of DNA complementary to chicken liver fatty acid synthase mRNA. Proc. Natl. Acad. Sci. USA 85:6328-6331.