메뉴 건너뛰기




Volumn 336, Issue 1, 2005, Pages 46-50

Denaturing RNA electrophoresis in TAE agarose gels

Author keywords

Electrophoresis; Formamide; Northern blotting; RNA

Indexed keywords

AMIDES; ELECTROPHORESIS; GELS;

EID: 9944244481     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.09.010     Document Type: Article
Times cited : (145)

References (22)
  • 1
    • 0017113951 scopus 로고
    • Specifity of oligo(dT)-cellulose chromatography in the isolation of polyadenylated RNA
    • J.M. Bantle, I.H. Maxwell, and W.E. Hahn Specifity of oligo(dT)-cellulose chromatography in the isolation of polyadenylated RNA Anal. Biochem. 72 1976 413 427
    • (1976) Anal. Biochem. , vol.72 , pp. 413-427
    • Bantle, J.M.1    Maxwell, I.H.2    Hahn, W.E.3
  • 2
    • 0000890081 scopus 로고
    • Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange
    • G.K. McMaster, and G.C. Carmichael Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange Proc. Natl. Acad. Sci. USA 74 1977 4835 4838
    • (1977) Proc. Natl. Acad. Sci. USA , vol.74 , pp. 4835-4838
    • McMaster, G.K.1    Carmichael, G.C.2
  • 3
    • 0019061278 scopus 로고
    • Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose
    • P.S. Thomas Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose Proc. Natl. Acad. Sci. USA 77 1980 5201 5204
    • (1980) Proc. Natl. Acad. Sci. USA , vol.77 , pp. 5201-5204
    • Thomas, P.S.1
  • 4
    • 0017281820 scopus 로고
    • Methylmercury as a reversible denaturing agent for agarose gel electrophoresis
    • J.M. Baily, and N. Davidson Methylmercury as a reversible denaturing agent for agarose gel electrophoresis Anal. Biochem. 70 1976 75 85
    • (1976) Anal. Biochem. , vol.70 , pp. 75-85
    • Baily, J.M.1    Davidson, N.2
  • 5
    • 0020096495 scopus 로고
    • Analytical gel electrophoresis of high-molecular-weight RNA in acrylamide-agarose gels containing methylmercuric hydroxide
    • T.J. Sebo, and J.C. Schmit Analytical gel electrophoresis of high-molecular-weight RNA in acrylamide-agarose gels containing methylmercuric hydroxide Anal. Biochem. 120 1982 136 145
    • (1982) Anal. Biochem. , vol.120 , pp. 136-145
    • Sebo, T.J.1    Schmit, J.C.2
  • 6
    • 0016076947 scopus 로고
    • Separation of single-stranded ribonucleic acids by acrylamide-agarose- urea gel electrophoresis
    • R.W. Floyd, M.P. Stone, and W.K. Joklik Separation of single-stranded ribonucleic acids by acrylamide-agarose-urea gel electrophoresis Anal. Biochem. 59 1974 599 609
    • (1974) Anal. Biochem. , vol.59 , pp. 599-609
    • Floyd, R.W.1    Stone, M.P.2    Joklik, W.K.3
  • 7
    • 0018345953 scopus 로고
    • RNase III cleaves vesicular stomatitis virus genome-length RNAs but fails to cleave viral mRNAs
    • G.W. Wertz, and N.L. Davis RNase III cleaves vesicular stomatitis virus genome-length RNAs but fails to cleave viral mRNAs J. Virol. 30 1979 108 115
    • (1979) J. Virol. , vol.30 , pp. 108-115
    • Wertz, G.W.1    Davis, N.L.2
  • 8
    • 0029123812 scopus 로고
    • A simple procedure for gel electrophoresis and northern blotting of RNA
    • S.K. Goda, and N.P. Minton A simple procedure for gel electrophoresis and northern blotting of RNA Nucleic Acids Res. 23 1995 3357 3358
    • (1995) Nucleic Acids Res. , vol.23 , pp. 3357-3358
    • Goda, S.K.1    Minton, N.P.2
  • 10
    • 0017283244 scopus 로고
    • Molecular-weight determination of animal-cell RNA by electrophoresis in formamide under fully denaturing conditions on exponential polyacrylamide gels
    • G. Spohr, M.-E. Mirault, T. Imaizumi, and K. Scherrer Molecular-weight determination of animal-cell RNA by electrophoresis in formamide under fully denaturing conditions on exponential polyacrylamide gels Eur. J. Biochem. 62 1976 313 322
    • (1976) Eur. J. Biochem. , vol.62 , pp. 313-322
    • Spohr, G.1    Mirault, M.-E.2    Imaizumi, T.3    Scherrer, K.4
  • 11
    • 0023872610 scopus 로고
    • Agarose gel electrophoresis of denatured RNA with silver staining
    • R.N. Skopp, and L.C. Lane Agarose gel electrophoresis of denatured RNA with silver staining Anal. Biochem. 169 1988 132 137
    • (1988) Anal. Biochem. , vol.169 , pp. 132-137
    • Skopp, R.N.1    Lane, L.C.2
  • 12
    • 0017643426 scopus 로고
    • RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination
    • H. Lehrach, D. Diamond, J.M. Wozney, and H. Boedtker RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination Biochemistry 16 1977 4743 4751
    • (1977) Biochemistry , vol.16 , pp. 4743-4751
    • Lehrach, H.1    Diamond, D.2    Wozney, J.M.3    Boedtker, H.4
  • 14
    • 0027465163 scopus 로고
    • Loss of resolution in gel electrophoresis of RNA: A problem associated with the presence of formaldehyde gradients
    • S.S. Tsang, X. Yin, C. Guzzo-Arkuran, V.S. Jones, and A.J. Davison Loss of resolution in gel electrophoresis of RNA: a problem associated with the presence of formaldehyde gradients BioTechniques 14 1993 380 381
    • (1993) BioTechniques , vol.14 , pp. 380-381
    • Tsang, S.S.1    Yin, X.2    Guzzo-Arkuran, C.3    Jones, V.S.4    Davison, A.J.5
  • 15
    • 0027159044 scopus 로고
    • Effects of staining of RNA with ethidium bromide before electrophoresis on performance of Northern blots
    • B. Ogretmen, H. Ratajczak, A. Kats, and B.C. Stark Effects of staining of RNA with ethidium bromide before electrophoresis on performance of Northern blots BioTechniques 14 1993 932 935
    • (1993) BioTechniques , vol.14 , pp. 932-935
    • Ogretmen, B.1    Ratajczak, H.2    Kats, A.3    Stark, B.C.4
  • 16
    • 9944263719 scopus 로고    scopus 로고
    • RNA integrity number (RIN)-standardization of RNA quality control
    • Publication Number 5989- 1165EN
    • O. Mueller, S. Lightfoot, A. Schroeder, RNA integrity number (RIN)-standardization of RNA quality control, Agilent Application Note, Publication Number 5989- 1165EN, 2004, pp. 1-8
    • (2004) Agilent Application Note , pp. 1-8
    • Mueller, O.1    Lightfoot, S.2    Schroeder, A.3
  • 18
    • 0023277545 scopus 로고
    • Single-step method of RNA isolation by acid guanidinium thiocyanate phenol chloroform extraction
    • P. Chomczynski, and N. Sacchi Single-step method of RNA isolation by acid guanidinium thiocyanate phenol chloroform extraction Anal. Biochem. 162 1987 159 159
    • (1987) Anal. Biochem. , vol.162 , pp. 159-159
    • Chomczynski, P.1    Sacchi, N.2
  • 19
    • 0035137667 scopus 로고    scopus 로고
    • Global and specific translational regulation in the genomic response of Saccharomyces cerevisiae to a rapid transfer from a fermentable to a nonfermentable carbon source
    • K.M. Kuhn, J.L. DeRisi, P.O. Brown, and P. Sarnow Global and specific translational regulation in the genomic response of Saccharomyces cerevisiae to a rapid transfer from a fermentable to a nonfermentable carbon source Mol. Cell. Biol. 21 2001 916 927
    • (2001) Mol. Cell. Biol. , vol.21 , pp. 916-927
    • Kuhn, K.M.1    Derisi, J.L.2    Brown, P.O.3    Sarnow, P.4
  • 21
    • 0026516892 scopus 로고
    • One-hour downward alkaline capillary transfer for blotting of DNA and RNA
    • P. Chomczynski One-hour downward alkaline capillary transfer for blotting of DNA and RNA Anal. Biochem. 201 1992 134 139
    • (1992) Anal. Biochem. , vol.201 , pp. 134-139
    • Chomczynski, P.1
  • 22
    • 0026654006 scopus 로고
    • Solubilization in formamide protects RNA from degradation
    • P. Chomczynski Solubilization in formamide protects RNA from degradation Nucleic Acids Res. 20 1992 3791 3791
    • (1992) Nucleic Acids Res. , vol.20 , pp. 3791-3791
    • Chomczynski, P.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.