ATP binding site mutagenesis reveals different subunit stoichiometry of functional P2X2/3 and P2X2/6 receptors

Journal of Biological Chemistry

Volumn 287, Issue 17, 2012, Pages 13930-13943

  Hausmann, Ralf ^a   Bodnar, Mandy ^b   Woltersdorf, Ronja ^a   Wang, Haihong ^b   Fuchs, Martin ^b   Messemer, Nanette ^b   Qin, Ying ^b   Günther, Janka ^a   Riedel, Thomas ^b   Grohmann, Marcus ^a,b   Nieber, Karen ^b   Schmalzing, Günther ^a   Rubini, Patrizia ^b   Illes, Peter ^b  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ATP-BINDING SITE; CO-EXPRESSION; HEK293 CELLS; IMAGING METHOD; INTRACELLULAR CA; NON-FUNCTIONAL; PATCH CLAMP TECHNIQUE; PROTEIN LABELING; TRANSMEMBRANE CURRENTS; XENOPUS LAEVIS OOCYTES;

AMINO ACIDS; ELECTROPHYSIOLOGY; ENZYME ACTIVITY; STOICHIOMETRY;

BINDING SITES;

2 METHYLTHIOADENOSINE TRIPHOSPHATE; ADENOSINE TRIPHOSPHATE; ALPHA,BETA METHYLENEADENOSINE TRIPHOSPHATE; PURINERGIC P2X2 RECEPTOR; PURINERGIC P2X3 RECEPTOR; PURINERGIC P2X6 RECEPTOR;

ANIMAL CELL; ARTICLE; BINDING SITE; CALCIUM CELL LEVEL; CALCIUM CURRENT; CELL STRAIN HEK293; CONTROLLED STUDY; ELECTROPHYSIOLOGY; ENZYME BINDING; FEMALE; GENE MUTATION; MOLECULAR RECOGNITION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS; STOICHIOMETRY; XENOPUS LAEVIS;

ADENOSINE TRIPHOSPHATE; ANIMALS; BINDING SITES; CALCIUM; DIMERIZATION; ELECTROPHYSIOLOGY; HEK293 CELLS; HUMANS; MUTAGENESIS; MUTATION; PATCH-CLAMP TECHNIQUES; PROTEIN BINDING; RECEPTORS, PURINERGIC P2; RECEPTORS, PURINERGIC P2X2; RECEPTORS, PURINERGIC P2X3; SIGNAL TRANSDUCTION; STRUCTURE-ACTIVITY RELATIONSHIP; SURFACE PROPERTIES; XENOPUS LAEVIS;

EID: 84859937532     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M112.345207     Document Type: Article

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