Choline and/or folic acid deficiency is associated with genomic damage and cell death in human lymphocytes in vitro

Nutrition and Cancer

Volumn 64, Issue 3, 2012, Pages 481-487

  Lu, Lin ^a,b   Ni, Juan ^a   Zhou, Tao ^a   Xu, Weijiang ^a   Fenech, Michael ^c   Wang, Xu ^a  

^a YUNNAN NORMAL UNIVERSITY   (China)

^b KUNMING MEDICAL UNIVERSITY   (China)

^c CSIRO   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

CHOLINE; FOLIC ACID;

ADULT; APOPTOSIS; ARTICLE; CELL DAMAGE; CELL DEATH; CELL DIVISION; CYTOTOXICITY; FEMALE; FOLIC ACID DEFICIENCY; GENOMIC INSTABILITY; HUMAN; HUMAN CELL; IN VITRO STUDY; LYMPHOCYTE; MALE; MICRONUCLEUS; VITAMIN DEFICIENCY;

ADULT; APOPTOSIS; CELL DEATH; CELL NUCLEUS; CELLS, CULTURED; CHOLINE; CHOLINE DEFICIENCY; DNA DAMAGE; FEMALE; FOLIC ACID; FOLIC ACID DEFICIENCY; GENOMIC INSTABILITY; HUMANS; LYMPHOCYTES; MALE; MICRONUCLEUS TESTS; MIDDLE AGED; NECROSIS;

EID: 84859746197     PISSN: 01635581     EISSN: 15327914     Source Type: Journal    
DOI: 10.1080/01635581.2012.660671     Document Type: Article

References (43)

1. Little, J, Sharp, L, Duthie, S, and, Narayanan, S. 2003. Colon cancer and genetic variation in folate metabolism: the clinical bottom line. J Nutr, 133: 3758S-3766S. (Pubitemid 37392597)
2. Ordovás, J M, Robertson, R, and, Cléirigh, E N. 2011. Gene-gene and gene-environment interactions defining lipid-related traits. Curr Opin Lipidol, 22: 129-136.
3. Ames, B N, and, Wakimoto, P. 2002. Are vitamin and mineral deficiencies a major cancer risk?. Nat Rev Cancer, 2: 694-704. (Pubitemid 37328920)
4. Ames, B N, Gold, L S, and, Willett, W C. 1995. The causes and prevention of cancer. Proc Natl Acad Sci U S A, 925: 258-5265.
5. Fenech, M. 2002. Micronutrients and genomic stability: a new paradigm for recommended dietary allowances (RDAs). Food Chem T, 40: 1113-1117. (Pubitemid 34626875)
6. Fenech, M. 2008. Genome health nutrigenomics and nutrigeneticsdiagnosis and nutritional treatment of genome damage on an individual basis. Food Chem T, 46: 1365-1370. (Pubitemid 351258195)
7. Fenech, M. 2010. Dietary reference values of individual micronutrients and nutriomes for genome damage prevention: current status and a road map to the future. Am J Clin Nutr, 91: 1438S-1454S.
8. Davis, C D, and, Uthus, E O. 2004. DNA methylation, cancer susceptibility, and nutrient interactions. Exp Biol Med, 229: 988-995. (Pubitemid 39431078)
9. Jang, H, Mason, J B, and, Choi, S W. 2005. Genetic and epigenetic interactions between folate and aging in carcinogenesis. J Nutr, 135: 2967S-2971S. (Pubitemid 43156574)
10. Sharp, L, Little, J, Schofield, A C, Pavlidou, E, Cotton, SC,. 2002. Folate and breast cancer: the role of polymorphisms in methylenetetrahydrofolate reductase (MTHFR). Cancer Lett, 181: 65-71. (Pubitemid 34310663)
11. Shinozuka, Y, Hasegawa, H, Ogawa, K, Tagoku, K, and, Hashimoto, T. 2006. Distinct effects of folate and choline deficiency on plasma kinetics of methionine and homocysteine in rats. Metabol, 55: 899-906. (Pubitemid 43902892)
12. Pufulete, M P, Emery, W, and, Sanders, A B. 2003. Folate, DNA methylation and colorectal cancer. PNS, 62: 437-445.
13. Duthie, S J, and, Hawdon, A. 1998. DNA instability (strand breakage, uracil misincorporation, and defective repair) is increased by folic acid depletion in human lymphocytes in vitro. FASEB J, 12: 1491-1497. (Pubitemid 28506718)
14. Crott, J W, Mashiyama, S T, Ames, B N, and, Fenech, M. 2001. The effect of folic acid deficiency and MTHFR C677T polymorphism on chromosome damage in human lymphocytes in vitro. Cancer Epidemiol Biomarkers Prev, 10: 1089-1096. (Pubitemid 32959157)
15. Crott, J W, Mashiyama, S T, Ames, B N, and, Fenech, M. 2001. MTHFR C677T polymorphism does not alter folic acid deficiency induced uracil incorporation into primary human lymphocyte DNA in vitro. Carcinogenesis, 22: 1019-1025. (Pubitemid 32633599)
16. Fenech, M. 2001. The role of folic acid and vitamin B12 in genomic stability of human cells. Mutat Res, 475: 56-67.
17. Beaudin, A E, and, Stover, P J. 2007. Folate-mediated one-carbon metabolism and tube defect: balancing genome synthesis and gene expression. Birth Defects Res C Embryo Today, 81: 183-203. (Pubitemid 350089908)
18. Ghoshal, K, Li, X, Datta, J, Bai, S, Pogribny, I. 2006. A folate- and methyl-deficient diet alters the expression of DNA methyltransferases and methyl CpG binding proteins involved in epigenetic gene silencing in livers of F344 rats. J Nutr, 136: 1522-1527. (Pubitemid 43765356)
19. Crott, J W, Liu, Z H, Keyes, M K, Choi, S-W, Jang, H. 2008. Moderate folate depletion modulates the expression of selected genes involved in cell cycle, intracellular signaling and folate uptake in human colonic epithelial cell lines. J Nutr Bio, 19: 328-335. (Pubitemid 351503765)
20. Wang, X, Thomas, P, Xue, J L, and, Fenech, M. 2004. Folate deficiency induces aneuploidy in human lymphocytes in vitroevidence using cytokinesis-blocked cells and probes specific for chromosome 17 and 21. Mut Res-, 551: 167-180. (Pubitemid 38833506)
21. Ni, J, Lu, L, Fenech, M, and, Wang, X. 2010. Folate deficiency in human peripheral blood lymphocytes induces chromosome 8 aneuploidy but this effect is not modified by riboflavin. Environ Mol Mutagen, 51: 15-22.
22. Beetstra, S, Thomas, P, Salisbury, C, Turner, J, and, Fenech, M. 2005. Folic acid deficiency increases chromosomal instability, chromosome 21 aneuploidy and sensitivity to radiation-induced micronuclei. Mut Res, 578: 317-326. (Pubitemid 41395335)
23. Zeisel, S H, and, Blusztajn, J K. 1994. Choline and human nutrition. Annu Rev Nutr, 14: 269-296. (Pubitemid 24261839)
24. Ueland, P M. 2011. Choline and betaine in health and disease. J Inherit Metab Dis, 34: 3-15.
25. Zeisel, S H. 2009. Importance of methyl donors during reproduction. Am J Clin Nutr, 89: 673S-677S.
26. Batra, V, and, Devasagayam, T P. 2009. Interaction between cytotoxic effects of gamma-radiation and folate deficiency in relation to choline reserves. Toxicology, 255: 91-99.
27. Craciunescu, C N, Johnson, A R, and, Zeisel, S H. 2010. Dietary choline reverses some, but not all, effects of folate deficiency on neurogenesis and apoptosis in fetal mouse brain. J Nutr, 140: 1162-1166.
28. James, S J, and, Yin, L. 1989. Diet-induced DNA damage and altered nucleotide metabolism in lymphocytes from ethyl-donor-deficient rats. Carcinogenesis, 10: 1209-1214. (Pubitemid 19177222)
29. da Costa, K A, Niculescu, M D, Craciunescu, C N, Fischer, L M, and, Zeisel, S H. 2006. Choline deficiency increases lymphocyte apoptosis and DNA damage in humans. Am J Clin Nutr, 84: 88-94. (Pubitemid 44699175)
30. Wang, X, and, Fenech, M. 2003. A comparison of folic acid and 5-methyltetrahydrofolate for prevention of DNA damage and cell death in human lymphocytes in vitro. Mutat Res, 18: 81-86. (Pubitemid 36118822)
31. Wang, X, Wu, X Y, Liang, Z Q, Huang, Y C, Fenech, M. 2006. A comparison of genomic instability under folic acid deficiency between breast cancer patients and normal non-cancer controls from a Chinese population in Yunnan. Mutagenesis, 21: 41-47. (Pubitemid 43288736)
32. Fenech, M. 2002. Chromosomal biomarkers of genomic instability relevant to cancer. Drug Disc T, 22: 1128-1137. (Pubitemid 35346420)
33. Fenech, M. 2000. The in vitro micronucleus technique. Mutat Res, 20: 81-95.
34. Fenech, M. 2007. Cytokinesis-block micronucleus cytome assay. N Prot, 77: 1084-1104. (Pubitemid 46901289)
35. Kimura, M, Umegak, K, Hihuchi, M, Thomas, P, and, Fenech, M. 2004. Methylenetetrahydrofolate reductase C677T polymorphism, folic acid and riboflavin are important determinants of genome stability in cultured human lymphocytes. J Nutr, 134: 48-56. (Pubitemid 38057422)
36. Fenech, M. 2010. Nutriomes and nutrient arrays: the key to personalised nutrition for DNA damage prevention and cancer growth control. Genome Integr, 1: 11
37. Jacob, R A, Jenden, D J, Allman-Farinelli, M A, and, Swendseid, M E. 1999. Folate nutriture alter choline status of women and men fed low choline diets. J Mutr, 129: 712-717. (Pubitemid 29118056)
38. Banni, S, Corongiu, F P, Dessi, M A, Lannone, A, Lombardi, B. 1989. Free radicals and lipid peroxidation in liver of rats kept on a diet devoid of choline. Free Radic Res Commun, 7: 233-240. (Pubitemid 19281971)
39. Niculesc, M D, da Costa, K A, Fischer, L M, and, Zeisel, S H. 2007. Lymphocyte gene expression in subjects fed a low-choline diet differs between those who develop organ dysfunction and those who do not. Am J Clin Nutr, 86: 230-239. (Pubitemid 47041615)
40. Ivanov, A, Nash-Barboza, S, Hinkis, S, and, Caudill, M A. 2009. Genetic variants in phosphatidylethanolamine N-methyltransferase and methylenetetrahydrofolate dehydrogenase influence biomarkers of choline metabolism when folate intake is restricted. J Am Diet Assoc, 109: 313-318.
41. Zeisel, S H. 2008. Genetic polymorphisms in methyl-group metabolism and epigenetics: lessons from humans and mouse models. Brain Res,: 1237-11. 5
42. Heil, S G, Lievers, K JA, Boers, G H, Verhoef, P, Heijer, M D. 2000. Betaine-homocysteine methyltransferase (BHMT): genomic sequencing and relevance to hyperhomocysteinemia and vascular disease in humans. Mol Genet Metab, 71: 511-519.
43. Wang, J A, Dudman, N P, Lynch, J, and, Wilcken, D E. 1991. Betaine homocysteine methyltransferasea new assay for the liver enzyme and its absence from human skin fibroblasts and peripheral blood lymphocytes. Clin Chimica Acta, 204: 239-249.