The in vitro micronucleus technique

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Volumn 455, Issue 1-2, 2000, Pages 81-95

  Fenech, Michael ^a  

^a CSIRO   (Australia)

Author keywords

Chromosome; DNA damage; Genetic toxicology

Indexed keywords

CYTARABINE; CYTOCHALASIN B; CYTOTOXIC AGENT; DNA;

APOPTOSIS; CARCINOGENESIS; CELL DEATH; CHROMOSOME BREAKAGE; CHROMOSOME DAMAGE; CHROMOSOME LOSS; CHROMOSOME MUTATION; CHROMOSOME REARRANGEMENT; CYTOTOXICITY; DNA DAMAGE; EXCISION REPAIR; GENETIC TOXICOLOGY; GENOTOXICITY; HUMAN; IN VITRO STUDY; MICRONUCLEUS TEST; MITOSIS INHIBITION; MOLECULAR PROBE; NONDISJUNCTION; NONHUMAN; PRIORITY JOURNAL; REVIEW;

EID: 0034693730     PISSN: 00275107     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0027-5107(00)00065-8     Document Type: Article

References (65)

1. Molecular mechanisms in the induction of chromosome aberrations
2. Update on target theory as applied to chromosomal aberrations
3. Cytogenet.: Overview
4. Aneuploidy and health risk assessment: Current status and future directions
5. Exclusion of specific human chromosomes into micronuclei by 5-azacytidine treatment of lymphocyte cultures
6. Mutagenicity testing with cultured mammalian cells: Cytogenetic assays
7. The micronucleus test
8. A rapid in vivo test for chromosome damage
9. Solutions to the kinetic problem in the micronucleus assay
10. Measurement of micronuclei in lymphocytes
11. Cytokinesis-block micronucleus method in human lymphocytes: Effect of in vivo ageing and low-dose x-irradiation
12. Effects of cytochalasins on mammalian cells
13. A method for the selective measurement of the radiosensitivity of quiescent cells in solid tumours-combination of immunofluorescence staining to BrdU and micronucleus assay
14. Micronucleus induction in cytokinesis-blocked mouse bone-marrow cells in vitro following in vivo exposure to X-irradiation and cyclophosphamide
15. Immunofluorescent staining of kinetochores in micronuclei: A new assay for the detection of aneuploidy
16. The identification of micronucleated chromosomes: A possible assay for aneuploidy
17. Use of fluorescence in situ hybridisation for the detection of aneugens in cytokinesis-blocked mouse splenocytes
18. Sex chromosomes, micronuclei and aging in women
19. Guidelines for testing of chemicals. Procedures for the detection of chemically induced aneuploidy: Recommendations of a UK Environmental Mutagen Society working group
20. Indications for a threshold of chemically induced aneuploidy in vitro in human lymphocytes
21. Conversion of excision-repairable DNA lesions to micronuclei within one cell cycle in human lymphocytes
22. Simultaneous detection of X-chromosome loss and non-disjunction in cytokinesis-blocked human lymphocytes by in situ hybridisation with a centromeric DNA probe; implications for the human lymphocyte in vitro micronucleus assay using cytochalasin-B
23. Indication for thresholds of chromosome non-disjunction versus chromosome lagging induced by spindle inhibitors in vitro in human lymphocytes
24. A critical evaluation of centromeric labelling to distinguish micronuclei induced by chromosomal loss and breakage in vitro
25. The in vitro micronucleus test: A multi-end-point assay to detect simultaneously mitotic delay, apoptosis, chromosome breakage, chromosome loss and non-disjunction
26. Necrosis, apoptosis, cytostasis and DNA damage in human lymphocytes measured simultaneously within the cytokinesis-block micronucleus assay: Description of the method and results for hydrogen peroxide
27. The CB in vitro Micronucleus Assay in Human Lymphocytes
28. A structural analysis of cytoskeletal components during the execution phase of apoptosis
29. Identification of aneuploidy-inducing agents using cytokinesis-blocked human lymphocytes and an antikinetochore antibody
30. The origin of micronuclei induced by cytosine arabinoside and its synergistic interaction with hydroxyurea in human lymphocytes
31. The suitability of the micronucleus assay in human lymphocytes as a new biomarker of excision repair
32. A collaborative study on the improvement of the micronucleus test in cultured human lymphocytes
33. The cytokinesis-block micronucleus assay as a biological dosimeter in spleen and peripheral blood lymphocytes in the mouse following acute whole body irradiation
34. Concurrent detection of gene mutations and chromosomal aberrations induced in vivo in somatic cells
35. Initiating carcinogen, triethylenemelamine, induces micronuclei in skin target cells
36. Measurement of micronuclei by cytokinesis-block method in cultured Chinese hamster cells: Comparison with types and rates of chromosome aberrations
37. Radiation induction of micronuclei in human lymphocytes
38. Induction of micronuclei and anaphase aberrations by cytochalasin-B in human lymphocyte cultures
39. Effect of cytochalasin-B on the induction of chromosome missegregation by colchicine at low concentrations in human lymphocytes
40. The advantages and disadvantages of the cytokinesis-block micronucleus method
41. Chemically induced micronucleus formation in V79 cells - Comparison of three different test procedures
42. Validation study of the in vitro micronucleus test in a Chinese hamster lung cell line (CHL/IU)
43. Mathematical model of the in vitro micronucleus assay predicts false negative results if micronuclei are not scored specifically in binucleated cells or cells that have completed one nuclear division
44. Sequence of centromere separation: Kinetochore formation in induced laggards and micronuclei
45. Three related centromere proteins are absent from the inactive centromere of a stable dicentric chromosome
46. Spindle poisons can induce polyploidy by mitotic slippage and micronucleate mononucleates in the cytokinesis-block assay
47. Distribution of kinetochore antigen in mammalian cell nuclei
48. Kinetochore detection in micronuclei: An alternative method for measuring chromosome loss
49. Imidazole increases the sensitivity of the cytochemical reaction for peroxidase with diaminobenzidine at neutral pH
50. Increased sensitivity in peroxidase immunochemistry. A comparative study of a number of peroxidase visualisation methods employing a model system
51. Genotoxic potential of tamoxifen and analogues in female Fischer F344/n rats, DBA/2 and C57BL/6 mice and in human MCL-5 cells
52. Biomarkers of DNA damage in marine mammals
53. A comparison of lymphocyte micronuclei and plasma micronutrients in vegetarians and non-vegetarians
54. Radiation-induced micronucleus induction in lymphocytes identifies a high frequency of radiosensitive cases among breast cancer patients: A test for predisposition?
55. Tumour radiosensitivity prediction by the cytokinesis-block micronucleus assay
56. The HUman MicroNucleus Project - An international collaborative study on the use of the micronucleus technique for measuring DNA damage in humans
57. The present state of the automated micronucleus test for lymphocytes
58. Automated detection of cytochalasin-B blocked binucleated lymphocytes for scoring micronuclei
59. Image processing algorithms for the automated micronucleus assay in binucleated human lymphocytes
60. Automatic analysis of the in vitro micronucleus test on V79 cells
61. Scoring of radiation-induced micronuclei in cytokinesis-blocked human lymphocytes by automated image analysis
62. Selective entrapment of extrachromosomally amplified DNA by nuclear budding and micronucleation during S phase
63. Gamma-irradiation-induced micronuclei from mouse hepatoma cells accumulate high levels of the tumour suppressor protein p53
64. Activation of p53-mediated cell cycle checkpoint in reponse to micronuclei formation