Combinatorial engineering of ldh-a and bcl-2 for reducing lactate production and improving cell growth in dihydrofolate reductase-deficient Chinese hamster ovary cells

Applied Microbiology and Biotechnology

Volumn 92, Issue 4, 2011, Pages 779-790

  Jeon, Min Kyoung ^a   Yu, Da Young ^a   Lee, Gyun Min ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Apoptosis; Bcl 2; CHO cells; LDH A; Specific lactate production rate

Indexed keywords

ANTI-APOPTOTIC PROTEIN; BCL-2; CELL LINES; CHINESE HAMSTER OVARY CELLS; CHO CELL; COMBINATORIAL ENGINEERING; CONTROL-CELL; DETRIMENTAL EFFECTS; DIHYDROFOLATE; GLUCOSE METABOLISM; HOST CELLS; LDH-A; PRODUCT FORMATION; PRODUCTION RATES; PYRUVATES; SPECIFIC LACTATE PRODUCTION RATE; THERAPEUTIC PROTEIN;

CELL DEATH; CELL GROWTH; GENETIC ENGINEERING; GLUCOSE; GROWTH KINETICS; PROTEINS;

CELL CULTURE;

DIHYDROFOLATE REDUCTASE; HYBRID PROTEIN; LACTATE DEHYDROGENASE; LACTIC ACID; PROTEIN BCL 2; PYRUVIC ACID; SMALL INTERFERING RNA;

APOPTOSIS; DISEASE TREATMENT; ENZYME ACTIVITY; GENE EXPRESSION; GENETIC ENGINEERING; GLUCOSE; GROWTH; PROTEIN; RECOMBINATION; RODENT;

ANIMAL CELL; ANIMAL EXPERIMENT; APOPTOSIS; ARTICLE; CELL GROWTH; CELL VIABILITY; CHO CELL; DOWN REGULATION; FEMALE; GENE OVEREXPRESSION; GENETIC ENGINEERING; GLUCOSE INTAKE; GLUCOSE METABOLISM; HOST CELL; MOUSE; NONHUMAN;

ANIMALS; APOPTOSIS; CELL SURVIVAL; CHO CELLS; CRICETINAE; CRICETULUS; GENE EXPRESSION; IMMUNOGLOBULIN FC FRAGMENTS; ISOENZYMES; L-LACTATE DEHYDROGENASE; LACTIC ACID; OXIDATION-REDUCTION; PROTO-ONCOGENE PROTEINS C-BCL-2; RECOMBINANT FUSION PROTEINS; TETRAHYDROFOLATE DEHYDROGENASE;

CRICETULUS GRISEUS;

EID: 82355176018     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3475-0     Document Type: Article

References (38)

1. C Altamirano A Illanes S Becerra JJ Cairo F Godia 2006 Considerations on the lactate consumption by CHO cells in the presence of galactose J Biotechnol 125 547 556 10.1016/j.jbiotec.2006.03.023 1:CAS:528:DC%2BD28XpsVWlsrc%3D (Pubitemid 44333124)
2. C Altamirano C Paredes A Illanes JJ Cairo F Godia 2004 Strategies for fed-batch cultivation of t-PA producing CHO cells: substitution of glucose and glutamine and rational design of culture medium J Biotechnol 110 171 179 10.1016/j.jbiotec.2004.02.004 1:CAS:528:DC%2BD2cXjs1Cltbg%3D (Pubitemid 38569671)
3. DC Andersen L Krummen 2002 Recombinant protein expression for therapeutic applications Curr Opin Biotechnol 13 117 123 10.1016/S0958-1669(02)00300-2 1:CAS:528:DC%2BD38Xis1Clt7s%3D (Pubitemid 34310369)
4. K Chen Q Liu L Xie PA Sharp DI Wang 2001 Engineering of a mammalian cell line for reduction of lactate formation and high monoclonal antibody production Biotechnol Bioeng 72 55 61 10.1002/1097-0290(20010105)72:1<55::AID-BIT8>3. 0.CO;2-4 1:CAS:528:DC%2BD3MXitVOktQ%3D%3D
5. Lmediates increased production of humanized monoclonal antibodies in Chinese hamster ovary cells Biotechnol Bioeng 91 779 792 10.1002/bit.20551 1:CAS:528:DC%2BD2MXhtVSkt77M (Pubitemid 41344680)
6. GM Cohen 1997 Caspases: the executioners of apoptosis Biochem J 326 Pt 1 1 16 1:CAS:528:DyaK2sXlvFChsL4%3D (Pubitemid 27386911)
7. H Dorai YS Kyung D Ellis C Kinney C Lin D Jan G Moore MJ Betenbaugh 2009 Expression of anti-apoptosis genes alters lactate metabolism of Chinese Hamster Ovary cells in culture Biotechnol Bioeng 103 592 608 10.1002/bit.22269 1:CAS:528:DC%2BD1MXmtlGqu7Y%3D
8. H Duan AM Chinnaiyan PL Hudson JP Wing WW He VM Dixit 1996 ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis J Biol Chem 271 1621 1625 10.1074/jbc.271.44.27863 1:CAS:528:DyaK28XlsVyntw%3D%3D (Pubitemid 26367363)
9. B Figueroa E Ailor D Osborne JM Hardwick M Reff MJ Betenbaugh 2007 Enhanced cell culture performance using inducible anti-apoptotic genes E1B-19K and Aven in the production of a monoclonal antibody with Chinese hamster ovary cells Biotechnol Bioeng 97 877 892 10.1002/bit.21222 1:CAS:528: DC%2BD2sXms1aku7w%3D (Pubitemid 47051057)
10. MW Glacken RJ Fleischaker AJ Sinskey 1986 Reduction of waste product excretion via nutrient control: Possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells Biotechnol Bioeng 28 1376 1389 10.1002/bit.260280912 1:CAS:528:DyaL28XlsFWntLY%3D (Pubitemid 16635886)
11. T Hassell S Gleave M Butler 1991 Growth inhibition in animal cell culture. The effect of lactate and ammonia Appl Biochem Biotechnol 30 29 41 10.1007/BF02922022 1:CAS:528:DyaK38Xot1WktA%3D%3D
12. G Hinterkörner G Brugger D Müller F Hesse R Kunert H Katinger N Borth 2007 Improvement of the energy metabolism of recombinant CHO cells by cell sorting for reduced mitochondrial membrane potential J Biotechnol 129 651 657 10.1016/j.jbiotec.2007.02.002 (Pubitemid 46590449)
13. D-W Jeong TS Kim JW Lee KT Kim HJ Kim I-H Kim IY Kim 2001 Blocking of acidosis-mediated apoptosis by a reduction of lactate dehydrogenase activity through antisense mRNA expression Biochem Biophys Res Commun 289 1141 1149 10.1006/bbrc.2001.6091 1:CAS:528:DC%2BD3MXptVWjtLw%3D (Pubitemid 34076310)
14. NS Kim GM Lee 2000 Overexpression of bcl-2 inhibits sodium butyrate-induced apoptosis in Chinese hamster ovary cells resulting in enhanced humanized antibody production Biotechnol Bioeng 71 184 193 10.1002/1097- 0290(2000)71:3<184::AID-BIT1008>3.0.CO;2-W 1:CAS:528:DC%2BD3MXjtFyqtrg%3D (Pubitemid 32381138)
15. NS Kim GM Lee 2002 Response of recombinant Chinese hamster ovary cells to hyperosmotic pressure: effect of Bcl-2 overexpression J Biotechnol 95 237 248 10.1016/S0168-1656(02)00011-1 1:CAS:528:DC%2BD38Xjtl2itrY%3D (Pubitemid 34465467)
16. SH Kim GM Lee 2007 Down-regulation of lactate dehydrogenase-A by siRNAs for reduced lactic acid formation of Chinese hamster ovary cells producing thrombopoietin Appl Microbiol Biotechnol 74 152 159 10.1007/s00253-006-0654-5 1:CAS:528:DC%2BD2sXhtVans7o%3D (Pubitemid 46183480)
17. YG Kim JY Kim C Mohan GM Lee 2009 Effect of Bcl-xL overexpression on apoptosis and autophagy in recombinant Chinese hamster ovary cells under nutrient-deprived condition Biotechnol Bioeng 103 757 766 10.1002/bit.22298 1:CAS:528:DC%2BD1MXnslejur8%3D
18. L overexpression does not enhance specific erythropoietin productivity of recombinant CHO cells grown at 33 degrees C and 37 degrees C Biotechnol Prog 25 252 256 10.1002/btpr.91 1:CAS:528:DC%2BD1MXivFehtr8%3D
19. MS Lao D Toth 1997 Effects of ammonium and lactate on growth and metabolism of a recombinant Chinese hamster ovary cell culture Biotechnol Prog 13 688 691 10.1021/bp9602360 1:CAS:528:DyaK2sXms1OitLY%3D (Pubitemid 27442057)
20. SK Lee GM Lee 2003 Development of apoptosis-resistant dihydrofolate reductase-deficient Chinese hamster ovary cell line Biotechnol Bioeng 82 872 876 10.1002/bit.10633 1:CAS:528:DC%2BD3sXjvFOqsLk%3D (Pubitemid 36604460)
21. SF Lim KH Chuan S Liu SOH Loh BYF Chung CC Ong Z Song 2006 RNAi suppression of Bax and Bak enhances viability in fed-batch cultures of CHO cells Metab Eng 8 509 522 10.1016/j.ymben.2006.05.005 1:CAS:528:DC%2BD28XhtVyitLrF (Pubitemid 44512161)
22. JA Lippke Y Gu C Sarnecki PR Caron MS Su 1996 Identification and characterization of CPP32/Mch2 homolog 1, a novel cysteine protease similar to CPP32 J Biol Chem 271 1825 1828 10.1074/jbc.271.4.1825 1:CAS:528: DyaK28XnsF2nsA%3D%3D (Pubitemid 26047759)
23. MA Medina I Nunez de Castro 1990 Glutaminolysis and glycolysis interactions in proliferant cells Int J Biochem 22 681 683 10.1016/0020-711X(90) 90001-J 1:CAS:528:DyaK3cXkvFGntLc%3D (Pubitemid 20374318)
24. FJ Oliver G de la Rubia V Rolli MC Ruiz-Ruiz G de Murcia JM Murcia 1998 Importance of poly(ADP-ribose) polymerase and its cleavage in apoptosis. Lesson from an uncleavable mutant J Biol Chem 273 33533 33539 10.1074/jbc.273.50.33533 1:CAS:528:DyaK1MXmvA%3D%3D (Pubitemid 29005737)
25. SS Ozturk BO Palsson 1990 Chemical decomposition of glutamine in cell culture media: effect of media type, pH, and serum concentration Biotechnol Prog 6 121 128 10.1021/bp00002a005 1:CAS:528:DyaK3cXhslKqurk%3D
26. DE Pascoe D Arnott ET Papoutsakis WM Miller DC Andersen 2007 Proteome analysis of antibody-producing CHO cell lines with different metabolic profiles Biotechnol Bioeng 98 391 410 10.1002/bit.21460 1:CAS:528:DC%2BD2sXhtVKlsr3J (Pubitemid 47518060)
27. JM Renard R Spagnoli C Mazier MF Salles E Mandine 1988 Evidence that monoclonal antibody production kinetics is related to the integral of the viable cells curve in batch systems Biotechnol Lett 10 91 96 10.1007/BF01024632
28. A Sanfeliu JJ Cairo C Casas C Sola F Godia 1996 Analysis of nutritional factors and physical conditions affecting growth and monoclonal antibody production of the hybridoma KB-26.5 cell line Biotechnol Prog 12 209 216 10.1021/bp950078x 1:CAS:528:DyaK28Xhs1ChtLo%3D (Pubitemid 126545765)
29. TM Sauerwald GA Oyler MJ Betenbaugh 2003 Study of caspase inhibitors for limiting death in mammalian cell culture Biotechnol Bioeng 81 329 340 10.1002/bit.10473 1:CAS:528:DC%2BD3sXktlylug%3D%3D (Pubitemid 36098489)
30. N Sengupta ST Rose JA Morgan 2011 Metabolic flux analysis of CHO cell metabolism in the late non-growth phase Biotechnol Bioeng 108 82 92 10.1002/bit.22890 1:CAS:528:DC%2BC3cXhsVCrtrjN
31. YH Sung GM Lee 2005 Enhanced human thrombopoietin production by sodium butyrate addition to serum-free suspension culture of Bcl-2-overexpressing CHO cells Biotechnol Prog 21 50 57 10.1021/bp049892n 1:CAS:528:DC%2BD2cXhtVagtLbK (Pubitemid 40218471)
32. YH Sung JS Lee SH Park J Koo GM Lee 2007 Influence of co-down-regulation of caspase-3 and caspase-7 by siRNAs on sodium butyrate-induced apoptotic cell death of Chinese hamster ovary cells producing thrombopoietin Metab Eng 9 452 464 10.1016/j.ymben.2007.08.001 1:CAS:528:DC%2BD2sXht1Gqu7nM (Pubitemid 350016972)
33. BT Tey RP Singh L Piredda M Piacentini M Al-Rubeai 2000 Influence of Bcl-2 on cell death during the cultivation of a Chinese hamster ovary cell line expressing a chimeric antibody Biotechnol Bioeng 68 31 43 10.1002/(SICI)1097- 0290(20000405)68:1<31::AID-BIT4>3.0.CO;2-L 1:CAS:528:DC%2BD3cXitVOru7Y%3D (Pubitemid 30163490)
34. GL Tritsch GE Moore 1962 Spontaneous decomposition of glutamine in cell culture media Exp Cell Res 28 360 364 10.1016/0014-4827(62)90290-2 1:CAS:528:DyaF3sXnsFGqsA%3D%3D
35. XH Wang J Xu Y Zhang L Li Q Feng L Mi ZN Chen 2004 Inducible expression of Bcl-XL inhibits sodium butyrate-induced apoptosis in hybridoma, resulting in enhanced antibody production Cell Biol Int 28 185 191 10.1016/j.cellbi.2003.11. 018 1:CAS:528:DC%2BD2cXhsV2hurs%3D (Pubitemid 38230229)
36. KF Wlaschin WS Hu 2007 Engineering cell metabolism for high-density cell culture via manipulation of sugar transport J Biotechnol 131 168 176 10.1016/j.jbiotec.2007.06.006 1:CAS:528:DC%2BD2sXos1Shtbk%3D (Pubitemid 47187511)
37. DCF Wong KTK Wong PM Nissom CK Heng MGS Yap 2006 Targeting early apoptotic genes in batch and fed-batch CHO cell cultures Biotechnol Bioeng 95 350 361 10.1002/bit.20871 1:CAS:528:DC%2BD28XpvFaitrw%3D (Pubitemid 44495370)
38. CY Yun S Liu SF Lim TH Wang BYF Chung JJ Teo KH Chuan ASC Soon KS Goh ZW Song 2007 Specific inhibition of caspase-8 and-9 in CHO cells enhances cell viability in batch and fed-batch cultures Metab Eng 9 406 418 10.1016/j.ymben.2007.06.001 1:CAS:528:DC%2BD2sXht1Gqu7jK (Pubitemid 350016969)