The cold-shock response in mammalian cells: Investigating the HeLa cell cold-shock proteome

Cytotechnology

Volumn 53, Issue 1-3, 2007, Pages 47-53

  Underhill, Michèle F ^a   Smales, C Mark ^a  

^a UNIVERSITY OF KENT   (United Kingdom)

Author keywords

2D PAGE; Bioprocessing; Cold shock; HeLa cells; Recombinant protein production; Sub physiological temperature culturing

Indexed keywords

COLD SHOCK PROTEIN; PROTEOME;

BIOPROCESS; CELL CULTURE; CELL GROWTH; CELL PROLIFERATION; CONFERENCE PAPER; FEMALE; HELA CELL; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IN VITRO STUDY; MAMMAL CELL; PROTEOMICS; TEMPERATURE DEPENDENCE;

MAMMALIA;

EID: 34250622016     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-007-9048-5     Document Type: Conference Paper

References (21)

1. Al-Fageeh MB, Marchant RJ, Carden MJ, Smales CM (2006) The cold-shock response in cultured mammalian cells: harnessing the response for the improvement of recombinant protein production. Biotechnol Bioeng 93:829-835
2. Al-Fageeh MB, Smales CM (2006) Control and regulation of the cellular responses to cold shock: the responses in yeast and mammalian systems. Biochem J 397:247-259
3. Baik JY, Lee MS, An SR, Yoon SK, Joo EJ, Kim YH, Park HW, Lee GM (2006) Initial transcriptome and proteome analyses of low culture temperature-induced expression in CHO cells producing erythropoietin. Biotechnol Bioeng 93:361-371
4. Beer C, Buhr P, Hahn H, Laubner D, Wirth M (2003) Gene expression analysis of murine cells producing amphotropic mouse leukaemia virus at a cultivation temperature of 32 and 37 degrees C. J Gen Virol 84:1677-1686
5. Ducommun P, Ruffieux PA, Kadouri A, von Stockar U, Marison I W (2002) Monitoring of temperature effects on animal cell metabolism in a packed bed process. Biotechnol Bioeng 77:838-842
6. Fogolin MB, Wagner R, Etcheverrigaray M, Kratje R (2004) Impact of temperature reduction and expression of yeast pyruvate carboxylase on hGM-CSF-producing CHO cells. J Biotechnol 109:179-191
7. Fountoulakis M, Tsangaris G, Oh JE, Maris A, Lubec G (2004) Protein profile of the HeLa cell line. J Chromatogr A 1038:247-265
8. Fox SR, Patel UA, Yap MG, Wang DIC (2004) Maximizing interferon-gamma production by Chinese hamster ovary cells through temperature shift optimization: experimental and modeling. Biotechnol Bioeng 85:177-184
9. Fox SR, Tan HK, Tan MC, Wong S, Yap MGS, Wang DIC (2005a) A detailed understanding of the enhanced hypothermic productivity of interferon-gamma by Chinese-hamster ovary cells. Biotechnol Appl Biochem 41:255-264
10. Fox SR, Yap MX, Yap MG, Wang DIC (2005b) Active hypothermic growth: a novel means for increasing total interferon-gamma production by Chinese-hamster ovary cells. Biotechnol Appl Biochem 41:265-272
11. Inouye S, Izu H, Takaki E, Suzuki H, Shirai M, Yokota Y, Ichikawa H, Fujimoto M, Nakai A (2004) Impaired IgG production in mice deficient for heat shock transcription factor 1. J Biol Chem 279:38701-38709
12. Kaufmann H, Mazur X, Fussenegger M, Bailey JE (1999) Influence of low temperature on productivity, proteome and protein phosphorylation of CHO cells. Biotechnol Bioeng 63:573-582
13. Moore A, Mercer J, Dutina G, Donahue CJ, Bauer KD, Mather JP, Etcheverry T, Ryll T (1997) Effects of temperature shift on cell cycle, apoptosis and nucleotide pools in CHO cell batch cultues. Cytotechnology 23:47-54
14. Ohsaka Y, Ohgiya S, Hoshino T, Ishizaki K (2002) Phosphorylation of c-Jun N-terminal kinase in human hepatoblastoma cells is transiently increased by cold exposure and further enhanced by subsequent warm incubation of the cells. Cell Physiol Biochem 12:111-118
15. Ramagli LS (1999) Quantifying protein in 2-D PAGE solubilization buffers. In: Link AJ (ed) Methods in molecular biology. 2-D proteome analysis protocols. Humana Press Inc, Totowa, NJ pp. 99-103
16. Schatz SM, Kerschbaumer RJ, Gerstenbauer G, Kral M, Dorner F, Scheiflinger F (2003) Higher expression of Fab antibody fragments in a CHO cell line at reduced temperature. Biotechnol Bioeng 84:433-438
17. Smales CM, Birch JR, Racher AJ, Marshall CT, James DC (2003) Evaluation of individual protein errors in silver-stained two-dimensional gels. Biochem Biophys Res Commun 306:1050-1055
18. Smales CM, Dinnis DM, Stansfield SH, Alete DE, Sage EA, Birch JR, Racher AJ, Marshall CT, James DC (2004) Comparative proteomic analysis of GS-NS0 murine myeloma cell lines with varying recombinant monoclonal antibody production rate. Biotechnol Bioeng 88:474-488
19. Underhill MF, Birch JR, Smales CM, Naylor LH (2005) eIF2alpha phosphorylation, stress perception, and the shutdown of global protein synthesis in cultured CHO cells. Biotechnol Bioeng 89:805-814
20. Yoon SK, Kim SH, Lee GM (2003a) Effect of low culture temperature on specific productivity and transcription level of anti-4-1BB antibody in recombinant Chinese hamster ovary cells. Biotechnol Prog 19:1383-1386
21. Yoon SK, Song JY, Lee GM (2003b) Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese hamster ovary cells. Biotechnol Bioeng 82:289-298