Two-dimensional difference fluorescence gel electrophoresis to verify the scale-up of a non-affinity-based downstream process for isolation of a therapeutic recombinant antibody

Electrophoresis

Volumn 31, Issue 11, 2010, Pages 1862-1872

  Grzeskowiak, Julita K ^a   Tscheliessnig, Anne ^b   Wu, Ming Wei ^b   Toh, Poh Choo ^b   Chusainow, Janet ^b   Lee, Yih Yean ^b   Wong, Niki ^b   Jungbauer, Alois ^a,b  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b AGENCY FOR SCIENCE   (Singapore)

Author keywords

2 D DIGE; IgG1; Non affinity based process; Purification; Recombinant monoclonal antibody; Scale up

Indexed keywords

CHROMATOGRAPHY; ELECTROPHORESIS; MONOCLONAL ANTIBODIES; RECOMBINANT PROTEINS;

2-D DIGE; DOWNSTREAM PROCESS; GEL ELECTROPHORESIS; IGG1; NON-AFFINITY-BASED PROCESS; PILOT SCALE; RECOMBINANT ANTIBODY; RECOMBINANT MONOCLONAL ANTIBODY; SCALE-UP; TWO-DIMENSIONAL;

PURIFICATION;

IMMUNOGLOBULIN G1 ANTIBODY; RECOMBINANT ANTIBODY; RHESUS ANTIBODY;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INTERMETHOD COMPARISON; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN PURIFICATION; SCALE UP; TWO DIMENSIONAL DIFFERENCE FLUORESCENCE GEL ELECTROPHORESIS; TWO DIMENSIONAL GEL ELECTROPHORESIS; WESTERN BLOTTING;

EID: 77952799809     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200900781     Document Type: Article

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