Separation of product associating E. coli host cell proteins OppA and DppA from recombinant apolipoprotein A-I milano in an industrial HIC unit operation

Biotechnology Progress

Volumn 25, Issue 2, 2009, Pages 446-453

  Hunter, Alan K ^a   Wang, Xing ^a   Suda, Eric J ^a   Herberg, John T ^a   Shell, Robert E ^a   Thomas, Kristin E ^a   Dufield, Robert L ^a   Gustafson, Mark E ^a   Mozier, Ned M ^a   Ho, Sa V ^a  

^a PFIZER INC   (United States)

Author keywords

Apolipoprotein A I Milano; HIC; Host cell protein; Scale up; Viscosity

Indexed keywords

APOLIPOPROTEIN A-I; APOLIPOPROTEIN A-I MILANO; CO-IMMUNOPRECIPITATION; COMPLEX PROBLEMS; CUT POINT; E. COLI; HIC; HOST CELL PROTEIN; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; KEY PARAMETERS; LABORATORY SCALE; MANUFACTURING PROCESS; PROCESS IMPROVEMENT; RESIN LOADING; SALT CONCENTRATION; SCALE-UP; SPECTROMETRY TECHNIQUE; TROUBLE SHOOTING; TWO-DIMENSIONAL GEL ELECTROPHORESIS; UNIT OPERATION; VISCOSITY EFFECTS; WESTERN BLOTTING;

CHROMATOGRAPHIC ANALYSIS; ELECTROPHORESIS; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GELATION; HYDROPHOBICITY; MASS SPECTROMETRY; PROTEINS; RESINS; VISCOSITY;

HYDROPHOBIC CHROMATOGRAPHY;

ESCHERICHIA COLI;

APOLIPOPROTEIN A I MILANO; APOLIPOPROTEIN A-I MILANO; APOLIPOPROTEIN A1; CARRIER PROTEIN; DPPA PROTEIN, E COLI; ESCHERICHIA COLI PROTEIN; LIPOPROTEIN; OPPA PROTEIN, E COLI; PERIPLASMIC BINDING PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; ESCHERICHIA COLI; EVALUATION; GENE EXPRESSION; GENETICS; HYDROPHOBICITY; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; METABOLISM; METHODOLOGY; MICROBIOLOGY;

APOLIPOPROTEIN A-I; CARRIER PROTEINS; CHROMATOGRAPHY, LIQUID; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; HYDROPHOBICITY; INDUSTRIAL MICROBIOLOGY; LIPOPROTEINS; PERIPLASMIC BINDING PROTEINS; RECOMBINANT PROTEINS;

EID: 66149181903     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1002/btpr.106     Document Type: Article

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