Use of proteomics for design of a tailored host cell for highly efficient protein purification

Journal of Chromatography A

Volumn 1216, Issue 12, 2009, Pages 2433-2438

  Liu, Zhu ^a   Bartlow, Patrick ^a   Varakala, Rajaramesh ^b   Beitle, Robert ^b   Koepsel, Richard ^a   Ataai, Mohammad M ^a  

^a University of Pittsburgh   (United States)

^b 3202 Bell Engineering Center   (United States)

Author keywords

Affinity tag; E. coli host cell protein contamination; GFP; Host cell design; Immobilized metal affinity chromatography; Protein purification; Proteomics

Indexed keywords

AFFINITY TAG; E. COLI HOST CELL PROTEIN CONTAMINATION; GFP; HOST CELL DESIGN; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; PROTEIN PURIFICATION; PROTEOMICS;

AFFINITY CHROMATOGRAPHY; CONTAMINATION; DESIGN; ESCHERICHIA COLI; IMPURITIES; METAL RECOVERY; METAL REFINING; MOLECULAR BIOLOGY; PROTEINS; PURIFICATION;

CHROMATOGRAPHIC ANALYSIS;

CELL PROTEIN; GREEN FLUORESCENT PROTEIN; HEXAHISTIDINE; NICKEL;

ARTICLE; BIOSEPARATION; CONTROLLED STUDY; ESCHERICHIA COLI; HOST CELL; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; KNOCKOUT GENE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN IMMOBILIZATION; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN TARGETING; PROTEOMICS;

CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE KNOCKOUT TECHNIQUES; GREEN FLUORESCENT PROTEINS; HISTIDINE; MUTATION; NICKEL; OLIGOPEPTIDES; PROTEINS; PROTEOMICS; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 60649091876     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2009.01.020     Document Type: Article

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