The establishment of an up-scaled micro-mixer method allows the standardized and reproducible preparation of well-defined plasmid/LPEI polyplexes

European Journal of Pharmaceutics and Biopharmaceutics

Volumn 77, Issue 1, 2011, Pages 182-185

  Kasper, Julia Christina ^a   Schaffert, David ^a   Ogris, Manfred ^a   Wagner, Ernst ^a   Friess, Wolfgang ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

Influence of concentration; Influence of mixing speed; Non viral gene delivery; Up scaled preparation; Well defined polyplexes

Indexed keywords

LUCIFERASE; PLASMID DNA; POLYETHYLENEIMINE;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; BATCH PROCESS; CHEMICAL PROCEDURES; COMPLEX FORMATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOMEGALOVIRUS; DNA TRANSFECTION; GENETIC TRANSFECTION; LIGHT SCATTERING; MOUSE; NEUROBLASTOMA CELL; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; REPRODUCIBILITY; UPSCALED MICROMIXER METHOD;

ANIMALS; BIOMEDICAL TECHNOLOGY; CELL LINE, TUMOR; GENE THERAPY; GENES, REPORTER; GENETIC VECTORS; MICE; NEUROBLASTOMA; OSMOLAR CONCENTRATION; PARTICLE SIZE; PLASMIDS; POLYETHYLENEIMINE; QUALITY CONTROL; TOXICITY TESTS; TRANSFECTION;

EID: 78650695686     PISSN: 09396411     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejpb.2010.11.012     Document Type: Article

References (11)

1. T.J. Anchordoquy, and G.S. Koe Physical stability of nonviral plasmid-based therapeutics J. Pharm. Sci. 89 2000 289 296
2. H. Talsma, J.-Y. Cherng, H. Lehrmann, M. Kursa, M. Ogris, W.E. Hennink, M. Cotten, and E. Wagner Stabilization of gene delivery systems by freeze-drying Int. J. Pharm. 157 1997 233 238 (Pubitemid 27471175)
3. E. Wagner Strategies to improve DNA polyplexes for in vivo gene transfer: will "artificial viruses" be the answer? Pharm. Res. 21 2004 8 14
4. J. Clement, K. Kiefer, A. Kimpfler, P. Garidel, and R. Peschka-Süss Large-scale production of lipoplexes with long shelf-life Eur. J. Pharm. Biopharm. 59 2005 35 43
5. O. Zelphati, C. Nguyen, M. Ferrari, J. Felgner, Y. Tsai, and P.L. Felgner Stable and monodisperse lipoplex formulations for gene delivery Gene Ther. 5 1998 1272 1282
6. D. Schaffert, M. Kiss, W. Rödl, A. Shir, A. Levitzki, M. Ogris, and E. Wagner Poly(I:C)-mediated tumor growth suppression in EGF-receptor overexpressing tumors using EGF-polyethylene glycol-linear polyethylenimine as carrier Pharm. Res. 2010 1 11
7. T. Mosmann Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays J. Immunol. Methods 65 1983 55 63
8. M.T. Kennedy, E.V. Pozharski, V.A. Rakhmanova, and R.C. MacDonald Factors governing the assembly of cationic phospholipid-DNA complexes Biophys. J. 78 2000 1620 1633
9. J.Y. Cherng, H. Talsma, R. Verrijk, D.J.A. Crommelin, and W.E. Hennink The effect of formulation parameters on the size of poly((2-dimethylamino)ethyl methacrylate)-plasmid complexes Eur. J. Pharm. Biopharm. 47 1999 215 224
10. J.G. Duguid, C. Li, M. Shi, M.J. Logan, H. Alila, A. Rolland, E. Tomlinson, J.T. Sparrow, and L.C. Smith A physicochemical approach for predicting the effectiveness of peptide-based gene delivery systems for use in plasmid-based gene therapy Biophys. J. 74 1998 2802 2814
11. C.A. Fernandez, and K.G. Rice Engineered nanoscaled polyplex gene delivery systems Mol. Pharm. 6 2009 1277 1289