A reporter gene assay for high-throughput screening of G-protein-coupled receptors stably or transiently expressed in HEK293 EBNA cells grown in suspension culture

Analytical Biochemistry

Volumn 284, Issue 2, 2000, Pages 316-326

  Durocher, Yves ^a   Perret, Sylvie ^a   Thibaudeau, Eric ^a   Gaumond, Marie Helene ^a   Kamen, Amine ^a   Stocco, Rino ^b   Abramovitz, Mark ^b  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b MERCK FROSST CENTRE FOR THERAPEUTIC RESEARCH   (Canada)

Author keywords

Antagonists; Cyclic AMP response element; Gene transcription; Prostanoid receptors; Secreted alkaline phosphatase

Indexed keywords

CELL CULTURE; DRUG PRODUCTS; PHOSPHATASES; SIGNAL TRANSDUCTION; VIRUSES;

ANTAGONISTS; GENE TRANSCRIPTIONS; PROSTANOID RECEPTORS; RESPONSE ELEMENTS; SECRETED ALKALINE PHOSPHATASE;

TRANSCRIPTION;

6 ISOPROPOXY 9 OXO 2 XANTHENECARBOXYLIC ACID; ALKALINE PHOSPHATASE PLACENTA ISOENZYME; CALCIUM IONOPHORE; CYCLIC AMP; G PROTEIN COUPLED RECEPTOR; PROSTANOID RECEPTOR;

ARTICLE; CELL CULTURE; CELL GROWTH; CYCLIC AMP RESPONSIVE ELEMENT; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; REPORTER GENE; SCREENING; SIGNAL TRANSDUCTION; TRANSCRIPTION REGULATION;

EID: 0034633263     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4698     Document Type: Article

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