The production of glycoproteins by transient expression in mammalian cells

Methods in Molecular Biology

Volumn 498, Issue , 2009, Pages 245-263

  Nettleship, Joanne E ^a   Rahman Huq, Nahid ^a   Owens, Raymond J ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Glycan; Glycoprotein; HEK 293T; Mammalian; Protein production; Protein purification; Transient

Indexed keywords

MAMMALIA;

EID: 84934443100     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-196-3_16     Document Type: Article

References (15)

1. Hammond, C., Braakman, I. and Helenius, A. (1994) Role of N-linked oligosaccharide recognition, glucose trimming, and calnexin in glycoprotein folding and quality control. Proceedings of the National Academy of Sciences of the United States of America, 91, 913-917.
2. Mitra, N., Sinha, S., Ramya, T.N.C. and Surolia, A. (2006) N-linked oligosaccharides as outfitters for glycoprotein folding, form and function. Trends in Biochemical Sciences, 31, 156-163.
3. Romanos, M.A., Scorer, C.A. and Clare, J.J. (1992) Foreign gene expression in yeast: a review. Yeast, 8, 423-488.
4. Yin, J., Li, G., Ren, X. and Herrler, G. (2007) Select what you need: a comparative evaluation of the advantages and limitations of frequently used expression systems for foreign genes. Journal of Biotechnology, 127, 335-347.
5. Harder, T.C. and Osterhaus, A.D.M.E. (1997) Molecular characterization and baculovirus expression of the glycoprotein B of a seal herpesvirus (Phocid Herpesvirus-1). Virology, 227, 343-352.
6. Kwong, P.D., Wyatt, R., Desjardins, E., Robinson, J., Culp, J.S., Hellmig, B.D., Sweet, R.W., Sodroski, J. and Hendrickson, W.A. (1999) Probability analysis of variational crystallization and its application to gp120, the exterior envelope glycoprotein of type 1 human immunodeficiency virus (HIV-1). The Journal of Biological Chemistry, 274, 4115-4123.
7. Wurm, F.M. (2004) Production of recombinant protein therapeutics in cultivated mammalian cells. Nature Biotechnology, 22, 1393-1398.
8. Durocher, Y., Perret, S. and Kamen, A. (2002) High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic Acids Research, 30, E9.
9. Wurm, F. and Bernard, A. (1999) Large-scale transient expression in mammalian cells for recombinant protein production. Current Opinion in Biotechnology, 10, 156-159.
10. Chang, V.T., Crispin, M., Aricescu, A.R., Harvey, D.J., Nettleship, J.E., Fennelly, J.A., Yu, C., Boles, K.S., Evans, E.J., Stuart, D.I. et al. (2007) Glycoprotein structural genomics: solving the glycosylation problem. Structure, 15, 267-273.
11. Berrow, N.S., Alderton, D., Sainsbury, S., Nettleship, J., Assenberg, R., Rahman, N., Stuart, D.I. and Owens, R.J. (2007) A versatile ligation-independent cloning method suitable for high-throughput expression screening applications. Nucleic Acids Research, 35, e45.
12. Aricescu, A.R., Lu, W. and Jones, E.Y. (2006) A time- and cost-efficient system for high-level protein production in mammalian cells. Acta Crystallographica Section D, 62, 1243-1250.
13. McCoy, J.P. Jr. (1987) The application of lectins to the characterization and isolation of mammalian cell populations. Cancer and Metastasis Reviews, 6, 595-613.
14. Nettleship, J.E., Brown, J., Groves, M.R. and Geerlof, A. (2007) In Kobe, B., Guss, M. and Huber, T. (eds.), Structural proteomics: High-throughput methods. Humana Press, Totowa, NJ.
15. (2002) Gel filtration: Principles and methods. AI ed. GE Healthcare, Uppsala, Sweden.