Expression system of CotA-laccase for directed evolution and high-throughput screenings for the oxidation of high-redox potential dyes

Biotechnology Journal

Volumn 4, Issue 4, 2009, Pages 558-563

  Brissos, Vânia ^a   Pereira, Luciana ^a   Munteanu, Florentina Daniela ^b   Cavaco Paulo, Artur ^b   Martins, Lígia O ^a  

^a INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^b UNIVERSITY OF MINHO   (Portugal)

Author keywords

Directed evoluti1on; Expression systems; High redox dyes; High throughput screening assays; Laccases

Indexed keywords

DIRECTED EVOLUTI1ON; EXPRESSION SYSTEMS; HIGH REDOX DYES; HIGH-THROUGHPUT SCREENING ASSAYS; LACCASES;

BACTERIOLOGY; CELL CULTURE; ESCHERICHIA COLI; SECONDARY BATTERIES;

THROUGHPUT;

ANTHRAQUINONIC DYE; AZO DYE; COTA LACCASE; DYE; LACCASE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; BACTERIUM CULTURE; BIOTECHNOLOGICAL PRODUCTION; CONTROLLED STUDY; DECOLORIZATION; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; GENE LIBRARY; HIGH THROUGHPUT SCREENING; NONHUMAN; OXIDATION REDUCTION POTENTIAL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SPECIES DIFFERENCE;

AEROBIOSIS; ANAEROBIOSIS; ANTHRAQUINONES; AZO COMPOUNDS; BACILLUS SUBTILIS; BACTERIAL PROTEINS; BENZOTHIAZOLES; COLORING AGENTS; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GENE EXPRESSION; GENE LIBRARY; KINETICS; LACCASE; MOLECULAR STRUCTURE; MUTATION; OXIDATION-REDUCTION; SULFONIC ACIDS; TEMPERATURE;

BACILLUS SUBTILIS; ESCHERICHIA COLI;

EID: 65549087033     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.200800248     Document Type: Article

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